We narrowed to 15,838 results for: grna

1. pLentiCRISPRv2 sgSp1

   Plasmid

   #174907

   Purpose

   Second generation sgRNA against Sp1

   Depositor

   Jane Clifford

   Article

   Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

   Insert

   Specificity Protein 1 (SP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Missense mutation to mutate PAM sequence

   Promoter

   EFS-NS

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CAG-mCherry-Otx2shRNA1

   Plasmid

   #73979

   Purpose

   Otx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   shRNA that targets the mouse Otx2 gene
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQ131D

   Plasmid

   #69287

   Purpose

   Golden Gate entry vector; 1st gRNA under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLKO-shTRAF2#2

   Plasmid

   #44128

   Depositor

   William Hahn

   Article

   Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

   Insert

   TRAF2 (TRAF2 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pLV.PARP1#5

   Plasmid

   #14548

   Depositor

   Didier Trono

   Article

   Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

   Insert

   PARP1 shRNA (PARP1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 27, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pRS423-TyrSgH

   Plasmid

   #163973

   Purpose

   Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

   Depositor

   Jiazhang Lian

   Article

   Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBrain-GFP-shGL2

   Plasmid

   #60004

   Purpose

   Co-expresses control GL2 shRNA and GFP.

   Depositor

   Stephen Royle

   Article

   Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

   Insert

   EGFP
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330A_FokI-1x2

   Plasmid

   #63602

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. plentiCRISPR-sgGOT1

   Plasmid

   #72874

   Purpose

   The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   sgGOT1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pMKO.1 GFP B56 gamma3 shRNA 14-1

    Plasmid

    #10681

    Depositor

    William Hahn

    Article

    Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

    Insert

    PP2A B56 gamma3 shRNA (PPP2R5C Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pLV.PARP1#4

    Plasmid

    #14547

    Depositor

    Didier Trono

    Article

    Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

    Insert

    PARP1 shRNA (PARP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2007

    Availability

    Academic Institutions and Nonprofits only

12. p458 VRER

    Plasmid

    #101728

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p330 VQR

    Plasmid

    #101730

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

    Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TET-pLKO.1 PURO shGFP

    Plasmid

    #83085

    Purpose

    Lentiviral shRNA vector for inducible knockdown of GFP (control hairpin)

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

15. pLentiCRISPRv2-sgLmnb2-HepmCherry

    Plasmid

    #192830

    Purpose

    Expresses sgRNA targeting Lmnb2 and mCherry from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for sgRNA and hepatocyte-specific promoter (HS…

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTAG1

    Plasmid

    #176863

    Purpose

    First sgRNA in a tRNA separated array of sgRNAs

    Depositor

    Daniel Voytas

    Article

    Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

    Insert

    sgRNA_1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMKO.1-Puro-shPP2A-Abeta

    Plasmid

    #15250

    Depositor

    William Hahn

    Article

    Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

    Insert

    PP2A regulatory subunit A, beta isoform (PPP2R1B Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

18. FUGW-H1-Syndecan-1 shRNA (ORF)

    Plasmid

    #40622

    Depositor

    Sally Temple

    Article

    Wang et al PLoS One. 2012;7(8):e42883. Epub 2012 Au

    Insert

    Syndecan-1 shRNA(ORF) (Sdc1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCBC-DT1T2.2

    Plasmid

    #134752

    Purpose

    PCR template for sgRNA cloning

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    sgRNA-AtU6_29p
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCBC-MT1T2.2

    Plasmid

    #134751

    Purpose

    PCR template for sgRNA cloning

    Depositor

    Qi-Jun Chen

    Article

    Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

    Insert

    sgRNA-TaU3p
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLKO.1-shRalA-1

    Plasmid

    #15257

    Depositor

    William Hahn

    Article

    Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

    Insert

    v-ral simian leukemia viral oncogene homolog A (RALA Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

22. petRNA-FANCF-v3'

    Plasmid

    #181803

    Purpose

    petRNA FANCF v3'

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

    Insert

    ribozyme-petRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. U6::sgBFP-U6::sgKrasG12D-pX333-red

    Plasmid

    #149448

    Purpose

    pX333 backbone expressing 2 sgRNA targeting BFP and KRAS G12D to edit mouse KRAS G12D allele.

    Depositor

    Jonathan Chernoff

    Article

    Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

    Insert

    sgRNA BFP and mouse KRAS G12D
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pX330A_D10A-1x4

    Plasmid

    #58774

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TET-pLKO.1 PURO shLacZ

    Plasmid

    #98632

    Purpose

    Lentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shLacZ
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFTK087

    Plasmid

    #171359

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    P-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAIO-LbCpf1

    Plasmid

    #137847

    Purpose

    LbCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Insert

    LbCpf1-NLS-FLAG
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBT009.J1.J306

    Plasmid

    #177325

    Purpose

    scRNA targeting J3 promoter for activation

    Depositor

    James Carothers

    Article

    Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

    Insert

    J306
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBT009.J3.RR2

    Plasmid

    #177326

    Purpose

    sgRNA targeting RFP for repression

    Depositor

    James Carothers

    Article

    Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

    Insert

    RR2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLKO.1-PRAS40

    Plasmid

    #15477

    Depositor

    Do-Hyung Kim

    Article

    Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

    Insert

    PRAS40 shRNA (AKT1S1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 21, 2007

    Availability

    Academic Institutions and Nonprofits only