177,628 results
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Plasmid#112895PurposeNon-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid librariesDepositorPublicationTypeEmpty backboneUseLentiviralPromoternoneAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA
Plasmid#99698PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAVDepositorTypeEmpty backboneUseAAVAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDGM3B
Plasmid#110809Purposecontains the AAV3B cap genes, AAV2 rep genes, and also adenovirus helper genesDepositorInsertAAV3B cap genes, AAV2 rep genes, adenovirus helper genes
UseAAVAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINE GFP nanobody:Halo:His6
Plasmid#111090PurposeBacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.DepositorInsertGFP Nanobody
TagsHalo and His6ExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-dCpf1-BE
Plasmid#107685PurposeExpresses dLbCpf1-BE in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdLbCpf1-BE
ExpressionMammalianMutationD832A/E925A/D1148APromoterCMVAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LentiV_Neo
Plasmid#108101PurposeLentiviral expression plasmid of cDNA with neomycin resistance gene (empty vector)DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsFLAG tagExpressionMammalianPromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-7 asyn A53T
Plasmid#105727PurposeBacterial expression of mutant human alpha synucleinDepositorAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.GCaMP6s.WPRE.SV40
Plasmid#100844PurposeAAV expression of ultrasensitive protein calcium sensor from CAG promoterDepositorHas serviceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV_hSyn1-SIO-stGtACR2-FusionRed
Plasmid#105677PurposeExpresses a soma-targeted GtACR2 in Cre-expressing neuronsDepositorHas serviceAAV1, AAV5, and AAV RetrogradeInsertstGtACR2
UseAAVTagsFusionRed and Kv2.1ExpressionMammalianPromoterhSyn1Available sinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSMPP-mCherry-mTrim21
Plasmid#104971PurposeLentiviral Transfer Plasmid with mCherry-tagged mouse Trim21DepositorAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pThermoCas9_ctrl
Plasmid#100981PurposeExpresses ThermoCas9 and its sgRNA moduleDepositorInsertsCas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module
TagsNoneExpressionBacterialPromoterB. coagulans DSM 1 pta promoter and B. smithii xy…Available sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetO-hNGN2-Neo
Plasmid#99378PurposeExpresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.DepositorAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSupAR-MbPylRS(DiZPK)
Plasmid#91705Purposeexpress Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interestDepositorInsertspylS
pylT
ExpressionBacterialMutationL274A, C313SAvailable sinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-DeAct-GS1
Plasmid#89445PurposeExpresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDeAct-GS1 (GSN Human)
TagsEGFPExpressionMammalianMutationTruncation spanning cytoplasmic Gsn amino acids 5…PromoterCMVAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-FlpO
Plasmid#89574PurposeExpresses FlpO under pCAGDepositorInsertFlpO
UseCre/Lox and Synthetic BiologyTagsNLSExpressionMammalianAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AICSDP-10:LMNB1-mEGFP
Plasmid#87422PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human LMNB1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNB1 Homology Arms with mEGFP-linker (LMNB1 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
BDNF-SEP
Plasmid#83955PurposeBDNF with pH sensitive fluorophore for visualization of releaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBrain derived neurotrophic factor (Bdnf Mouse)
TagsSEPExpressionMammalianAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pK036.TRE-Flpe-WPRE (Supernova)
Plasmid#85007PurposeFor Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.DepositorInsertNLS-FLPe-WPRE
ExpressionMammalianAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pME phluorin2-gpi
Plasmid#73793Purposeratiometric pHluorin2 with a gpi linkage sequence in a middle entry cloneDepositorInsertratiometric pHluorin2-gpi
UseMiddle entry cloneAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZW1-snoVector
Plasmid#73174PurposeNuclear expression of RNAsDepositorInsertsExpressionMammalianPromoterCMV promoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by