We narrowed to 22,696 results for: Sis;

1. MSCV-mNINJ1-K45Q-GFP

   Plasmid

   #208777

   Purpose

   MSCV vector expressing K45Q mouse NINJ1

   Depositor

   Petr Broz

   Article

   Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

   Insert

   NINJ1 (Ninj1 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mutation

   Change Lys 45 to Gln (K45Q)

   Promoter

   Available Since

   Aug. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCK083 desmaMCS:minpromEGFP,exorh:mCherry

   Plasmid

   #200016

   Purpose

   Expression construct with desmin a regulatory elements, mouse beta-globin minimal promoter EGFP, exorh:mCherry

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEG302 22aa SunTag NtDRMcd gRNA4 (FWA)

   Plasmid

   #115486

   Purpose

   CRISPR-Cas9 SunTag system to target NtDRMcd to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX301-TagRFP

   Plasmid

   #162035

   Purpose

   Tagged vector control

   Depositor

   David Bryant

   Article

   Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   RFP

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVenus-N-SLC35B2

   Plasmid

   #155038

   Purpose

   Transient expression of SLC35B2-Venus

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   SLC35B2 (SLC35B2 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   CMV

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTpal-fdc

   Plasmid

   #78288

   Purpose

   Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

   Depositor

   David Nielsen

   Article

   McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

   Inserts

   PAL2 (PAL2 Mustard Weed)
   FDC1 (FDC1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   *see below

   Promoter

   trc

   Available Since

   June 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. CRY2olig(FL)-mCherry

   Plasmid

   #60033

   Purpose

   Expresses a fusion of full length CRY2 (E490G) with mCherry

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

   Insert

   CRY2 E490G (deltaNLS) (CRY2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E490G, NLS mutated

   Promoter

   CMV

   Available Since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3_2FLAG-AKAP79ΔCN

   Plasmid

   #183451

   Purpose

   Expression of human AKAP79 lacking the calcineurin binding site (Δ337-343)

   Depositor

   Matthew Gold

   Article

   Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

   Insert

   AKAP5 (AKAP5 Human)
   

   Use

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   aa337-343 deleted

   Promoter

   CMV

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB545

   Plasmid

   #74879

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available Since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CMV-NGLY1-Puro

    Plasmid

    #181916

    Purpose

    Lentiviral plasmid that directs the expression of NGLY1 in mammalian cells.

    Depositor

    Scott Dixon

    Article

    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    N-glycanase 1 (NGLY1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. p8103 LentiCRISPR v2 sgNT-2

    Plasmid

    #163313

    Purpose

    Expresses Cas9 and a non-targeting control guide RNA

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    sgNT-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRN110

    Plasmid

    #127228

    Purpose

    CmYLCV::STM::35S terminator

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    STM (STM A. tumefaciens, Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRetroSuper-GFP Smad2

    Plasmid

    #15722

    Depositor

    Joan Massague

    Article

    He et al Cell. 2006 Jun 2. 125(5):929-41.

    Insert

    Smad2 shRNA (SMAD2 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-ATG8(414)/GFP-AUT7(414)

    Plasmid

    #49424

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.

    Depositor

    Daniel Klionsky

    Article

    Abeliovich et al Mol Biol Cell. 2003 Feb;14(2):477-90.

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    ATG8

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pET-mBoNT/F

    Plasmid

    #168015

    Purpose

    Expresses MBP-BoNT/F-LC(1-428)-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    MBP-BoNT/F-LC(1-428)-6xHis
    

    Use

    Tags

    Hisx6 and maltose binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJMP1333

    Plasmid

    #119268

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHLP700

    Plasmid

    #203742

    Purpose

    C-terminal tagging of target proteins with AID*/3xHA degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain codons 71-114
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    none

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHLP701

    Plasmid

    #203743

    Purpose

    C-terminal tagging of target proteins with 3xV5-IAA17 degron

    Depositor

    Mark Hall

    Article

    Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

    Insert

    IAA17 auxin binding domain
    

    Use

    Pcr amplification template for gene tagging

    Tags

    3xV5

    Expression

    Mutation

    Promoter

    none

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYFP-NGAT

    Plasmid

    #11383

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGWB521

    Plasmid

    #74863

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRetroSuper-shSrc

    Plasmid

    #26982

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    shRNA Src (SRC Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

22. pMSCV-WT-mAbl

    Plasmid

    #69027

    Purpose

    Retroviral vector encoding WT mouse c-Abl

    Depositor

    Jean Wang

    Article

    Sridevi et al PLoS One. 2013 Oct 11;8(10):e77495. doi:

    Insert

    mouse Abl-Type IV (wild type)
    

    Use

    Retroviral

    Tags

    FLAG tag inserted at Internal SalI site (2934 bp)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pCAGGS-Flag-hsDicer (D1709A)

    Plasmid

    #41588

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D1709A

    Promoter

    CMV

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

24. p7274 MSCV-P C-FlagHA 16 ∆DLYC

    Plasmid

    #163304

    Purpose

    Expresses HPV16 E7 ∆DLYC

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

    Insert

    HPV16 E7 ∆DLYC
    

    Use

    Retroviral

    Tags

    FlagHA

    Expression

    Mutation

    ∆DLYC

    Promoter

    MSCV LTR

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40

    Plasmid

    #179117

    Purpose

    Expresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytes

    Depositor

    Baljit Khakh

    Article

    Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

    Insert

    Fermt2 sgRNA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. shLacZ

    Plasmid

    #42559

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    shLacZ
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA5/FRT/TO HSPA1L

    Plasmid

    #19457

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA1L (HSPA1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHis2-Arf1 (17-181)-Q71L

    Plasmid

    #184808

    Purpose

    Bacterial expression of Arf1 (aa17-181, Q71L mutation)

    Depositor

    James Hurley

    Article

    Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

    Insert

    Arf1 (ARF1 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    aa 17-181 only, Q71L

    Promoter

    T7

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. YFP-Cdc42(N17)

    Plasmid

    #11400

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    cell division cycle 42 (CDC42 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Threonine 17 changed to Asparagine.

    Promoter

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. YFP-mGCN5-C1

    Plasmid

    #134573

    Purpose

    Expresses YFP-GCN5 fusion protein

    Depositor

    Susan Janicki

    Article

    Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

    Insert

    GCN5 (Kat2a Mouse)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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