We narrowed to 11,014 results for: phen

Showing: 10591 - 10620 of 11014 results

Results per page:

Sort results by:

GABA(A) receptor subunit g2SE

Plasmid

#49170

Purpose

pHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

Depositor

Tija Jacob, Stephen Moss

Article

Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.

Insert

GABA(A) receptor subunit gamma 2 (Gabrg2 Mouse)

Tags

myc, pHGFP (pHluorin), and thrombin cleavage site…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE HA Chk2 KD, dominant negative chk2

Plasmid

#41902

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

Chk2 (CHEK2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

D347A

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pPbcas13b

Plasmid

#89906

Purpose

Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

Depositor

Feng Zhang

Article

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

Insert

Cas13b

Expression

Bacterial

Promoter

Lac

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GABA(A) receptor subunit B3SE

Plasmid

#49171

Purpose

pHluorin-tagged GABA A receptor subunit (beta 3) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

Depositor

Tija Jacob, Stephen Moss

Article

Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.

Insert

GABA(A) receptor subunit beta 3 (Gabrb3 Mouse)

Tags

myc, pHGFP (pHluorin), and thrombin cleavage site…

Expression

Mammalian

Mutation

GTG (V) to TGC (C)

Promoter

CMV

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pHochTypeIII

Plasmid

#214039

Purpose

Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

Cas10

Use

CRISPR

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MBP-CHC(1-1074)-His6

Plasmid

#100748

Purpose

Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CLTC (CLTC Human)

Tags

His and MBP

Expression

Bacterial

Mutation

Residues 1-1074

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-His6-SUMO-MproC145A

Plasmid

#172653

Purpose

Bacterial expression of SARS-CoV-2 Mpro C145A inactive mutant fused to SUMO

Depositor

Stephen Harrison

Article

MacDonald et al ACS Infect Dis. 2021 Sep 10;7(9):2591-25

Insert

SARS-CoV-2 Mpro (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

6xHis Tag and SUMO

Expression

Bacterial

Mutation

Catalytic Cysteine 145 mutated to Alanine

Promoter

T7

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-DHFR F[1,2]-C (TRP1)

Plasmid

#177795

Purpose

Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[1,2]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas-dCas12m

Plasmid

#192275

Purpose

Encodes MmdCas12m (D485A) under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmdCas12m

Use

CRISPR

Expression

Bacterial

Mutation

D485A

Promoter

PJ23108

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
[pSK24] pACYCDuet His-c7orf59|HBXIP

Plasmid

#136143

Purpose

bacterial expression of c7orf59 and HBXIP

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

c7orf59 and HBXIP (LAMTOR4, LAMTOR5 Human)

Expression

Bacterial

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-HemmarR

Plasmid

#31222

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdTom (CD4 Human, Aequorea victoria, Fly)

Tags

CD4 and tdTomato

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-LBR(1-245)-GFP

Plasmid

#222425

Purpose

To express a truncated LBR (encoding amino acids 1-245) with N-terminal FKBP tag accessible to the cytoplasm and C-terminal GFP in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

LBR(1-245aa) (LBR Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Truncation (Includes aa 1-245)

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

Plasmid

#229828

Purpose

A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER-21-RUNX1-P2A-ERG

Plasmid

#97045

Purpose

Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-beta2(616-951)-GFP

Plasmid

#100726

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER-21-HOXA9

Plasmid

#97041

Purpose

Expresses HOXA9 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

HOXA9 (HOXA9 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGM32DEST

Plasmid

#74747

Purpose

Encodes for the QF-GR recombinant gene, splice linker, and mCherry reporter gene

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

Gateway cassette
QF-GR (NR3C1 Neurospora crassa, Human, Synthetic)

Tags

Fusion of the Neurospora crassa QF activator to t…

Expression

Worm

Promoter

No promoter

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGART-2XStrep

Plasmid

#125681

Purpose

expresses human GART protein with a C-terminal 2XStrep affinity tag

Depositor

Stephen Benkovic

Article

Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

Insert

GART (GART Human)

Tags

2X Strep-Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-HemmarG

Plasmid

#31221

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdGFP (CD4 Human, Aequorea victoria, Fly)

Tags

CD4 and EGFP/GFP

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-DHFR F[1,2]-N (TRP1)

Plasmid

#177797

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Tags

DHFR F[1,2]

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER-21-HOXA5

Plasmid

#97040

Purpose

Expresses HOXA5 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Homeobox A5 (HOXA5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRLSIN.cPPT.PGK.MD(ND2bHLH).WPRE

Plasmid

#122055

Purpose

Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain

Depositor

Stephen Tapscott

Article

Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

Insert

MD(ND2bHLH) (Myod1, Neurod2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

MyoD chimeric protein substituted with the NeuroD…

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE MDER

Plasmid

#13494

Depositor

Stephen Tapscott

Article

Hollenberg et al Proc Natl Acad Sci U S A. 1993 Sep 1. 90

Insert

MyoD1-Estrogen Receptor Fusion Protein (Myod1 Human, Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

full length MyoD was fused to aa282-595 of Esr1. …

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pBZCas13b-HEPN

Plasmid

#89901

Purpose

Bacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.

Depositor

Feng Zhang

Article

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

Insert

Cas13b

Expression

Bacterial

Mutation

R116A/H121A/R1177A/H1182A

Promoter

Lac

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-beta3(702-1094)-GFP

Plasmid

#100734

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP3B1 (AP3B1 Human)

Tags

FKBP and GFP

Expression

Mammalian

Mutation

appendage and ear only

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP-beta2(616-951)-mRuby2

Plasmid

#100730

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2B1 (AP2B1 Human)

Tags

FKBP and mRuby2

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available Since

Sept. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
CD8-pTagRFP657-LOVpep (T406A, T407A, I532A)

Plasmid

#100742

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CD8A (CD8A Human)

Tags

LOVpep and tagRFP657

Expression

Mammalian

Mutation

Truncated

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
SBC009876

Plasmid

#226283

Purpose

Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

Depositor

Nigel Scrutton

Article

Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

Inserts

Coniferyl alcohol acyltransferase
Eugenol synthase 1

Expression

Bacterial

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2-CMV/TetO2-EYFP-GW

Plasmid

#153309

Purpose

Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid

Depositor

Spyros Petrakis

Article

Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

Type

Empty backbone

Use

Transposon

Tags

Yellow Fluorescent Protein

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5 FRT TO Myc ArB WT RNAiR

Plasmid

#59806

Purpose

Allows the integration of Myc ArB in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora B (AURKB Human)

Tags

Myc

Expression

Mammalian

Mutation

RNAi resistant

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge