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pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI

Plasmid

#115267

Purpose

Expresses LbuCas13a from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

LbuCas13a

Tags

SUMO

Expression

Bacterial

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-hSyn-dLight1.2

Plasmid

#111068

Purpose

To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter

Depositor

Lin Tian

Publication

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has service

AAV1 and AAV5

Insert

dLight1.2

Use

AAV

Tags

Flag tag

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pR_LG lenti-(empty)-del-CTS

Plasmid

#112895

Purpose

Non-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid libraries

Depositor

Paul Blainey

Publication

Feldman et al bioRxiv 262121

Type

Empty backbone

Use

Lentiviral

Promoter

none

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEMT-PTE2A

Plasmid

#111773

Purpose

Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

Depositor

Li Qian

Publication

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Type

Empty backbone

Use

Cloning intermediate

Promoter

T7

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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VEGFR2-mCh

Plasmid

#108854

Purpose

Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker

Depositor

Kalina Hristova

Publication

Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

Insert

KDR (KDR Human)

Tags

Labeled with mCherry on the C-Terminus via a 3 am…

Expression

Mammalian

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV_hSyn1-SIO-stGtACR2-FusionRed

Plasmid

#105677

Purpose

Expresses a soma-targeted GtACR2 in Cre-expressing neurons

Depositor

Ofer Yizhar

Publication

Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

Has service

AAV Retrograde, AAV1, and AAV5

Insert

stGtACR2

Use

AAV

Tags

FusionRed and Kv2.1

Expression

Mammalian

Promoter

hSyn1

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

43 Articles

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pCAG G-CEPIA1er

Plasmid

#105012

Purpose

ER calcium sensor

Depositor

Franck Polleux

Publication

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

G-CEPIA1er

Expression

Mammalian

Promoter

pCAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV-HDR-mEGFP-camk2a

Plasmid

#104589

Purpose

AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse camk2a

Depositor

Ryohei Yasuda

Publication

Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

None

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMH-SFB

Plasmid

#99391

Purpose

Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)

Depositor

Michael Huen

Publication

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCSC-NGN2-IRES-GFP-T2A-Sox11

Plasmid

#90214

Purpose

To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Publication

Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

Insert

NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Human, Mouse)

Use

Lentiviral

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-i53

Plasmid

#92170

Purpose

recombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1

Depositor

Daniel Durocher

Publication

Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

Insert

i53

Use

AAV

Tags

FLAG

Expression

Mammalian

Mutation

UbvG08 with I44A mutation and no terminal Glycines

Promoter

CMV

Available since

July 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-HF-BE3

Plasmid

#87439

Purpose

Expresses HF-BE3 with C-terminal NLS in mammalian cells

Depositor

David Liu

Publication

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Bacillus phage PBS2, Human, Rat)

Expression

Mammalian

Mutation

Mammalian codon-optimized Cas9-HF

Promoter

CMV

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab8a-wt

Plasmid

#86075

Purpose

A mammalian expression plasmid encoding Rab8a wild type with N-terminus EGFP.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCAG-FlpO

Plasmid

#89574

Purpose

Expresses FlpO under pCAG

Depositor

Wilson Wong

Publication

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO

Use

Cre/Lox and Synthetic Biology

Tags

NLS

Expression

Mammalian

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pT7-VP4SA11

Plasmid

#89165

Purpose

Express Rotavirus SA11 VP4 in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP4

Expression

Mammalian

Promoter

T7 promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pT7-VP2SA11

Plasmid

#89163

Purpose

Express Rotavirus SA11 VP2 in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP2

Expression

Mammalian

Promoter

T7 promotor

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pT7-VP1SA11

Plasmid

#89162

Purpose

Express Rotavirus SA11 VP1 in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP1

Expression

Mammalian

Promoter

T7 promotor

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEGFP-Akt1(WT)

Plasmid

#86637

Purpose

Expresses eGFP-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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BDNF-SEP

Plasmid

#83955

Purpose

BDNF with pH sensitive fluorophore for visualization of release

Depositor

Ryohei Yasuda

Publication

Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

Insert

Brain derived neurotrophic factor (Bdnf Mouse)

Tags

SEP

Expression

Mammalian

Available since

Feb. 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAVS1-Nst-CAG-DEST

Plasmid

#80489

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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