We narrowed to 14,249 results for: TIM
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Plasmid#107459PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G56A conferring wildtype phenotypeDepositorAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only
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pALPS_3'LTR GFP@gag start SFFV tat
Plasmid#101338PurposeLTR drives GFP and internal SFFV promoter drives codon optimized tatDepositorInsertGFP, tat
UseLentiviralAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro
Plasmid#89994PurposeDonor template for generation of NGN3-ntdTomato reporter cell linesDepositorAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSR35
Plasmid#69256Purposephsp16.41::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0Promoterhsp16.41Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin F463A
Plasmid#45975DepositorInsertParkin F463A (Prkn Rat)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromotertacAvailable SinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCSN069
Plasmid#166728PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-Mxi1
UseCRISPRTagsMxi1ExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSR28
Plasmid#69253Purposephlh-8::CRE for integration on ttTi14024, Chr XDepositorInsertCre recombinase
UseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0Promoterhlh-8Available SinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJAID5VY
Plasmid#165048PurposeIntegrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…Available SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK3FS-PYK1
Plasmid#85777PurposeN-ICE plasmid pK3FS-PYK1DepositorInsertloxP-KanMX-loxP-PYK1p-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET29NK-LGK/mGFPmut3
Plasmid#81168PurposeGFP fusion to levoglucosan kinaseDepositorInsertLevoglucosan kinase wild-type
TagsHis6x and mGFPmut3ExpressionBacterialPromoterT7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
JH05
Plasmid#73516PurposemYPet_PH pCFJ150 - contain C. elegans codon optimized mYPetDepositorInsertpmex-5::mPYet::PH::tbb-2
TagsmYPetPromotermex-5Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291
Plasmid#129671PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneExpressionPlantMutationBthCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-C1V1(t/t)-mRuby2-KV2.1-WPRE-SV40
Plasmid#124604PurposeCre-dependent soma-localized C1V1(t/t) for optogenetic stimulationDepositorInsertSoma-localized C1V1(t/t)
UseAAVTagsmRuby2ExpressionMammalianPromoterEf1aAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D570A
Plasmid#129673PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b-d570a entry …ExpressionPlantMutationD570A; AacCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEG584
Plasmid#40191DepositorInsertMex-5 (RNAi resistant) (mex-5 Nematode)
TagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationExon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable SinceSept. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_J23101-CocE
Plasmid#128129PurposeExpressing CocE enzyme (cocaine to benzoate) gene under control of the constitutive promoter J23101, and RBS B0032DepositorInsertCocE
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C3orf17 sgRNA 1
Plasmid#70652PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C3orf17 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C3orf17
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKARζ
Plasmid#119918PurposeMammalian expression of the tAKARζ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARζ
ExpressionMammalianAvailable SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLN426 (FuGW-G5p-mKate2)
Plasmid#105183PurposeModule 3 - GAL4 synthetic promoter (with five binding sites) drives mKate2 expressionDepositorInsertG5p-mKate2
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C9orf114 sgRNA 1
Plasmid#70655PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C9orf114
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - C9orf114 sgRNA 2
Plasmid#70654PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a C9orf114 targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.DepositorInsertsgRNA against C9orf114
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin F146A
Plasmid#45973DepositorInsertParkin F146A (Prkn Rat)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…PromotertacAvailable SinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSALECTNK-LGK/csTEM1
Plasmid#81164PurposepSALECT plasmid with levoglucosan kinase fused to TEM-1DepositorInsertLevoglucosan kinase
TagsCodon swapped TEM-1 beta-lactamase (H26 to W290) …ExpressionBacterialPromoterlacAvailable SinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEG587
Plasmid#40196DepositorInsertMex-5 (S458A) (RNAi resistant) (mex-5 Nematode)
TagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationS458A; exon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable SinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Dgcr8_3
Plasmid#73527PurposeExpresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8DepositorInsertDGCR8
UseCRISPRExpressionMammalianPromoterhU6Available SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Dgcr8_4
Plasmid#73528PurposeExpresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8DepositorInsertDGCR8
UseCRISPRExpressionMammalianPromoterhU6Available SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Dgcr8_2
Plasmid#73526PurposeExpresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8DepositorInsertDGCR8
UseCRISPRExpressionMammalianPromoterhU6Available SinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMA3200
Plasmid#46855PurposeA lentiviral vector encoding a fusion between codon-optimized P.aeruginosa exoY and myc-tagged destabilized FKBP12DepositorInsertExoY
UseLentiviralTagsFKBP E31G, R71G, K105E and MycPromoterTRE-TightAvailable SinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEG585
Plasmid#40192DepositorInsertMex-5 (S404A) (RNAi resistant) (mex-5 Nematode)
TagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationS404A; exon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable SinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-THP-Synaptophysin-pHluorin
Plasmid#242903PurposeFluorescent reporter for imaging of synaptic vesicle recycling in Dopaminergic NeuronsDepositorInsertsynaptophysin plus pHluorin
UseAAVTagsmouse synaptophysinExpressionMammalianPromoterrat Tyrosine hydroxylaseAvailable SinceJan. 14, 2026AvailabilityAcademic Institutions and Nonprofits only