We narrowed to 18,135 results for: URE

Showing: 10681 - 10710 of 18135 results

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Psicheck2 Sox4 mut 3'UTR oligo

Plasmid

#26988

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

Sox4 mut UTR oligo (SOX4 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Mutation

bp 449-509 of SOX4 3'UTR; mut @ bp 483

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pBS-AMV-β1L9'S-nAchR

Plasmid

#20685

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

nicotinic acetylcholine receptors (Chrnb1 Mouse)

Tags

AMV and pA 50

Expression

Bacterial

Mutation

Leucine at 9' position in M2 region changed …

Available Since

April 3, 2009

Availability

Academic Institutions and Nonprofits only
pAP2

Plasmid

#41755

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc E. coli, Fly)

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C45S C338S K349C C451S

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TYK2

Plasmid

#67133

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TYK2 (TYK2 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

A435S

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
HA SEK AL pcDNA3.1 zeo (+) - SEK1 (A-L)

Plasmid

#24653

Depositor

Jim Woodgett

Article

Yan et al Nature. . 372(6508):798-800.

Insert

SEK1 (MAP2K4 Human)

Tags

HA

Expression

Mammalian

Mutation

Ser220Ala, Thr224Leu

Available Since

June 21, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pET151-GFP-PP-RPN2 (918-932)

Plasmid

#73748

Purpose

Expression of GFP-PP-RPN2 (918-932) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 918-932 of RPN2

Promoter

T7

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR sgFTCD_1

Plasmid

#102312

Purpose

genetic depletion of FTCD

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgFTCD_1 (FTCD Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR sgFTCD_2

Plasmid

#102313

Purpose

genetic depletion of FTCD

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgFTCD_2 (FTCD Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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TDK promoter dCas9

Plasmid

#113657

Purpose

dCas9 expression unit plasmid. The dCas9 expression unit plasmids contain connector ConL1, ConRE, and one dCas9 transcriptional unit.

Depositor

Jeffrey Barrick

Article

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

TDK gene promoter and dCas9

Use

CRISPR

Expression

Bacterial

Promoter

TDK gene promoter

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCSD_SpCas9MT3-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107322

Purpose

Expresses SpCas9 (R1335K) fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1335K) is fused to SaCas9

Promoter

CMV IE93

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1‐Afu rnhB

Plasmid

#108694

Purpose

For expression in E coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII

Depositor

Andrew Jackson, Martin Reijns

Article

Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

Insert

rnhB (AF_RS03165 Archaeoglobus fulgidus)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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M26I DJ-1 TEV site pET15b

Plasmid

#60685

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26I DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26I and TEV site was inserted (see comments belo…

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pIS50 KIF2 3'UTR

Plasmid

#14493

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

KIF2 3'UTR (KIF2A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only

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eGFP-pk-N273K

Plasmid

#115617

Purpose

Expresses human Parkin N273K in mammalian cells

Depositor

Edward Fon

Article

Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

Insert

Parkin (PRKN Human)

Tags

EGFP

Expression

Mammalian

Mutation

N273K

Promoter

CMV

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Cwc25-pRSETA

Plasmid

#61043

Purpose

Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.

Depositor

Nils Walter

Article

Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

Insert

Cwc25

Tags

TEV site, Histidine Tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.2 mir-1-1 reporter hsa-mir17~20

Plasmid

#46671

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

hsa-mir17~20 (MIR17 Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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RCAS (B) flag Lunatic Fringe (CT#471)

Plasmid

#13980

Depositor

Cliff Tabin

Article

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Lunatic Fringe (LFNG Chicken)

Use

Retroviral; Avian expression

Tags

Flag

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 BMPR2

Plasmid

#67127

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BMPR2 (BMPR2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
tdEos-VASP-5

Plasmid

#57685

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

VASP

Tags

tdEos

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pRVH1-hygro shStx3

Plasmid

#40071

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Stx3 shRNA-1

Use

Retroviral

Promoter

H1

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
KAPCA_HUMAN_D0

Plasmid

#79741

Purpose

This plasmid encodes the kinase domain of KAPCA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KAPCA

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
M26L DJ-1 TEV site pET15b

Plasmid

#60686

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26L DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26L and TEV site was inserted (see comments belo…

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
RCAS (A) flag Lunatic Fringe (CT#479)

Plasmid

#13979

Depositor

Cliff Tabin

Article

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Lunatic Fringe (LFNG Chicken)

Use

Retroviral; Avian expression

Tags

Flag

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
Tip alpha K102A/K104A (21-192)

Plasmid

#23159

Depositor

SeWon Suh

Article

Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

Insert

TNF-α-inducing protein

Tags

His

Expression

Bacterial

Mutation

changed lysine 102 and lysine 104 to alanine, and…

Available Since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
pBlue-hFX(AA88)-HA

Plasmid

#15402

Depositor

Michael Ristow

Article

Dhe-Paganon et al J Biol Chem. 2000 Oct 6. 275(40):30753-6

Insert

human frataxin cDNA (FXN Human)

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pBS-AMV-γ1L9'S-nAchR

Plasmid

#20686

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

nicotinic acetylcholine receptors (Chrng Mouse)

Tags

AMV and pA 50

Expression

Bacterial

Mutation

Leucine at 9' position in M2 region changed …

Available Since

April 3, 2009

Availability

Academic Institutions and Nonprofits only
pYSD344

Plasmid

#253044

Purpose

Encodes 2xFLAG-tagged Aga2 with Protein A ZZ domain as a C-terminal fusion in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker

Depositor

Paul Young

Article

Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102

Insert

Aga2 Protein A

Expression

Yeast

Available Since

May 5, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 N-HA SIRT2-H150Y

Plasmid

#249435

Purpose

Used for transfection of cells for expression of N-HA SIRT2-H150Y

Depositor

Sheng-cai Lin

Article

Sun et al Nat Metab. 2025 Oct 22. doi: 10.1038/s42

Insert

SIRT2 (SIRT2 Human)

Tags

3xHA

Expression

Mammalian

Mutation

H150Y

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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Wild-type-ThermoCas9-3xNLS

Plasmid

#254685

Purpose

Expresses Wild-type ThermoCas9 with 3x NLS tags. Utilized for RNP transfection

Depositor

Hong Li

Article

Roth et al Nature. 2026 Apr 15. doi: 10.1038/s41586

Insert

Wildtype ThermoCas9

Tags

NLS, SV40 NLS, and nucleoplasmin NLS

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pLV_CMV_mEmerald-KDEL

Plasmid

#251341

Purpose

ER lumen targeted mEmerald, Lentiviral backbone, CMV promoter.

Depositor

Edward Avezov

Article

Parutto et al Nat Commun. 2026 Feb 11;17(1):2639. doi:

Insert

mEmerald

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2026

Availability

Academic Institutions and Nonprofits only

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