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pET21-pelB-LaRIgG-1

Plasmid

#228995

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaMIgG-5

Plasmid

#228991

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pNS38-SadCas9-SNAP

Plasmid

#113718

Purpose

Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

Depositor

Gerald Schwank

Article

Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

Insert

Cas9

Use

CRISPR

Tags

HA-2xNLS-SNAP-NLS and His6-MBP-TEV

Expression

Bacterial

Mutation

D10A, N580A

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-LivePARBackbone

Plasmid

#176526

Purpose

Expression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePAR

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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InsRA-TagBFP

Plasmid

#79791

Purpose

Insulin receptor isoform A with a inter-domain TagBFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

InsRA (INSR Human)

Tags

TagBFP extracellular

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-Tag4/hL3MBTL1

Plasmid

#28231

Depositor

Danny Reinberg

Article

Trojer et al Cell. 2007 Jun 1. 129(5):915-28.

Insert

l(3)mbt-like 1 (Drosophila) (L3MBTL1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Irrelevant S49T mutation

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only
pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

Plasmid

#138295

Purpose

gRNA for CRISPR/Cas9 knockout of human TRIM21

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

gRNA TRIM21 (TRIM21 Human)

Use

CRISPR

Promoter

U6

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pC129: pAAV.CMV-CasRx mCh

Plasmid

#203442

Purpose

Plasmid expressing active RfxCas13d with mCherry reporter for investigating CasRx activity

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Insert

RfxCas13d-T2A-mCherry

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pICSL11060

Plasmid

#68264

Purpose

Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAW-CD8-Nb127D01-GFP

Plasmid

#171577

Purpose

Expresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembrane

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

CD8-Nb127D01-GFP (CXCR2 Human)

Tags

CD8 and GFP

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-iGECI-NES

Plasmid

#160424

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI-NES

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-eGFP-SV40p-BlasR

Plasmid

#228271

Purpose

pLenti-CMV-eGFP-SV40p-BlasR plasmid for ectopic expression of GFP

Depositor

Judy Lieberman

Article

Fontana et al Cell. 2024 Sep 2:S0092-8674(24)00898-5.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-MHL: nsp16_SARS-CoV-2

Plasmid

#167850

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp16

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp16

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-DIO-Bmal1

Plasmid

#239045

Purpose

Overexpression of Bmal1

Depositor

Qingchun Tong

Article

Kim et al Nat Commun. 2020 Jul 30;11(1):3794. doi:

Insert

mArntl (Bmal1 Mouse)

Use

AAV

Promoter

Bmal1

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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K15-EGFP

Plasmid

#44266

Depositor

George Cotsarelis

Article

Morris et al Nat Biotechnol. 2004 Apr;22(4):411-7. Ep

Insert

K15 promoter (-4.8) (Krt15 Mouse)

Use

Mouse Targeting; Fluorescence microscopy

Tags

EGFP

Expression

Mammalian

Mutation

Contains the murine K15 promoter (-4.8) fragment

Promoter

murine K15 promoter (-4.8) fragment

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only
pX330.sgNf1.4

Plasmid

#92028

Purpose

pX330 sgNf1.4 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.4

Use

Mouse Targeting

Expression

Mammalian

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only
pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S

Plasmid

#62460

Purpose

expresses C151S and C288S double mutant human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteines 151 and 288 of Keap1 to respect…

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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A3G-BE5.13

Plasmid

#163637

Purpose

C to T DNA base editing

Depositor

Xue Gao

Article

Lee et al Sci Adv. 2020 Jul 15;6(29):eaba1773. doi

Insert

A3G-BE5.13

Tags

BPSV40 NLS

Expression

Mammalian

Mutation

See manuscript

Promoter

CMV

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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LLP773_pLenti-6xHRBKET-sgRNA

Plasmid

#211766

Purpose

Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)

Use

Lentiviral

Promoter

pCMV and U6

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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shLacZ

Plasmid

#42559

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shLacZ

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only