We narrowed to 12,359 results for: NSI

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pGP-CMV-NES-jRGECO1a

Plasmid

#61563

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRGECO1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-srIκBα

Plasmid

#242411

Purpose

Self-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (E3, NSs, L*) and inflammatory signaling inhibitor, super repressor inhibitor of κBα (srIκBα).

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

mScarlet3
IRES-E3-NSs-L*-EGFP
IRES-srIκBα

Use

Mouse Targeting; Self-amplifying rna

Expression

Mammalian

Mutation

serines 32 and 36 to alanines

Available Since

Sept. 25, 2025

Availability

Academic Institutions and Nonprofits only

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CAG sHRPb-NLG

Plasmid

#73144

Purpose

Small fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPb-NLG (Nlgn1 Synthetic, Mouse)

Tags

V5 tag

Expression

Mammalian

Promoter

CAG

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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EFS-SpdCas9

Plasmid

#210703

Purpose

This plasmid express EFS promoter driven human codon optimized SpdCas9

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

S. Pyogenes dCas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a core promoter

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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alpha catenin conformation sensor

Plasmid

#71709

Purpose

This alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.

Depositor

Deborah Leckband

Article

Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

Insert

alpha-catenin FRET Sensor

Tags

ECFP and YPET

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT_FLAG-Mx PylRS

Plasmid

#140011

Purpose

Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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Lentiviral-TOP-dGFP-reporter

Plasmid

#14715

Purpose

Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

LEF-1/TCF-responsive promoter (Ctnnb1 )

Use

Lentiviral

Tags

d2 eGFP

Expression

Mammalian

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pHR_PGK 3XFlag_4D5-5_CAR-mCherry

Plasmid

#247508

Purpose

Expresses a CAR against HER2 and mCherry

Depositor

Wendell Lim

Article

Simic et al Science. 2024 Dec 6;386(6726):eadl4237.

Insert

CAR against Her2 and mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-Digda

Plasmid

#236102

Purpose

Lysosome-targeted FRET-based biosensor for monitoring diacylglycerol (DAG) dynamics in living cells.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

Lyso-Digda (LAMP1 Synthetic, Human)

Tags

Full-length LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRCaMP1a

Plasmid

#61562

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRCaMP1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST-HisMBP-SED1

Plasmid

#178191

Purpose

SED1 biosensor for expression in Escherichia coli

Depositor

Cesar Cuevas-Velazquez, Jose Dinneny

Article

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 biosensor

Tags

6xHis-MBP

Expression

Bacterial

Promoter

tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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DPYD-FLAG

Plasmid

#61616

Purpose

The human DPYD was used to study the effect of DPYD expression on EMT

Depositor

David Sabatini

Article

Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

Insert

Dihydropyrimidine dehydrogenase (DPYD Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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LAMP1-mRFP-FLAG

Plasmid

#34611

Purpose

3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag

Depositor

David Sabatini

Article

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

2xFlag and mRFP

Expression

Mammalian

Available Since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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p426-Cup1p-FusionRed

Plasmid

#188392

Purpose

Cu2+-dependent expression of red fluorescent protein in yeast cells

Depositor

Michihiko Kataoka

Article

Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

Insert

FusionRed

Expression

Yeast

Promoter

CUP1 promoter

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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EGFR L858R

Plasmid

#11012

Purpose

Retroviral construct for expressing human EGFR with L858R mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR L858R (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L858R (tumor-derived)

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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Nrf1-Myc pcDNA3.1(+)-C-Myc (NAWLVH106-AAAAAA)

Plasmid

#169126

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hNrf1 (NFE2L1 Human)

Expression

Mammalian

Mutation

NAWLVH106-AAAAAA

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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Integrin β3-GFP

Plasmid

#205091

Purpose

Express GFP-tagged human integrin β3 in mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

Integrin beta 3 (ITGB3 Human)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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SG3ΔENV CA Q67H/N74D

Plasmid

#217442

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q67H/N74D mutations in capsid (CA Q67H/N74D).

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Mutation

CA Q67H,N74D

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pBW_0001

Plasmid

#102833

Purpose

Binary vector containing Sr22 Schomburgk allele driven by maize ubiquitin promoter, Sr22 terminator

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

Sr22 Schomburgk (NEWENTRY Synthetic)

Expression

Plant

Promoter

Maize ubiquitin

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pSEMS-CV SUy-Halo7Tag

Plasmid

#108933

Purpose

Selflabeling tag at ATP Synthase

Depositor

Karin Busch

Article

Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

Insert

ATP synthase F1 subunit gamma (ATP5F1C Human)

Tags

Halo7 tag

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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