We narrowed to 59,208 results for: Ran

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pSL0374 (His-MBP-Cas8_Cas7_Cas6)

Plasmid

#130639

Purpose

Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCas8, VchCas7, VchCas6

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on Cas8

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMS1: pHelper(AvCAST)_AvPSP1

Plasmid

#168133

Purpose

Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAC5-dual-dCas9VP48-sgTetO

Plasmid

#48237

Purpose

Dual expression construct expressing both dCas9VP48 and sgTetO from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9VP48 and sgTetO

Use

CRISPR

Tags

HA-tag and VP48

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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mRuby2-H2B-C-10

Plasmid

#55898

Purpose

Localization: Nucleus/Histones, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

H2B (H2BC11 Human)

Tags

mRuby2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pDGB1alpha2_SulR

Plasmid

#186425

Purpose

transcriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter

Depositor

Lukas Fischer

Article

Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

Insert

SulR

Use

Synthetic Biology; Binary vector for escherichia …

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b-eGO-Matryoshka-LE-LP

Plasmid

#100017

Purpose

Bacterial expression of fluorescent protein casette - cpEGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

cpEGFP-LSSmOrange

Tags

6x HIS tag

Expression

Bacterial

Mutation

Leu and Glu N-term linker and Leu and Pro C-term …

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV Luc2 (FF-Luciferase) L5-L2

Plasmid

#62168

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and firefly luciferase module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Luciferase

Use

Luciferase; Mule gateway entry vector

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-sgGAL4-4

Plasmid

#46916

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgGAL4-4
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV and U6

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pLINE1-scFvFc

Plasmid

#206020

Purpose

An intact type LINE-1 vector plasmid possessing the scFv-Fc expression unit

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

mouse LINE1 vector harboring an scFv-Fc expression unit

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3HA-ErbB4CTF

Plasmid

#17803

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

HA

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 676-1292

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pFL-EGFP-3xFlag-Fml1

Plasmid

#193058

Purpose

Baculovirus expression of S.pombe Fml1 for recombinant protein production

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

Fml1 (fml1 Fission Yeast)

Tags

3xFlag

Expression

Insect

Promoter

polyhedron

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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DNM2 delta PRD

Plasmid

#168275

Purpose

Express PRD-deleted DNM2 mutant (aa 1-742) in pEGFP-N1 vector.

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

DNM2 (DNM2 Human)

Tags

GFP

Expression

Mammalian

Mutation

aa 1-742

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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plc242-GFP-ITFG2

Plasmid

#87049

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-Tmx4-V5

Plasmid

#120236

Purpose

Expresses V5-tagged Tmx4 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Tmx4 (Tmx4 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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promoter J23110 (upstream of RBS, mRFP1)

Plasmid

#117852

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

medium-high promoter, RBS, mRFP1

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

Plasmid

#47454

Purpose

epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

CRY2PHR-NLS-SID4X

Use

AAV

Tags

2A_phiLOV2.1

Expression

Mammalian

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pSVE Grb2 myc 49L/203R

Plasmid

#26087

Depositor

Bob Weinberg

Article

Egan et al Nature. 1993 May 6. 363(6424):45-51.

Insert

Grb2 49L/203R (GRB2 Human)

Tags

myc

Expression

Mammalian

Mutation

49L/203R

Available Since

Sept. 23, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ROR1-V5/HIS

Plasmid

#205607

Purpose

Expression of human ROR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

ROR1 receptor tyrosine kinase (ROR1 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTYF-2xSYN-tTA

Plasmid

#19982

Depositor

Sergey Kasparov

Article

Liu et al J Gene Med. 2008 May . 10(5):583-92.

Insert

2xSYN-tTA (SYN1 E.coli, Human, Budding Yeast)

Expression

Mammalian

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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TMEM-bio-His

Plasmid

#51856

Purpose

Expresses full-length Transmembrane protein 109 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

TMEM (TMEM109 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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