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173,901 results

Showing: 10741 - 10770 of 173901 results

  1. pcDNA3-Lyn-dInPAkt

    Plasmid
    #181834
    Purpose
    Genetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).
    Depositor
    Jin Zhang
    Article
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Insert
    Lyn-dInPAkt
    Tags
    N-terminal targeting sequence from Lyn kinase, dd…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181834-plasmid-map-sequence-id-364316

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  2. ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1

    Plasmid
    #187459
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187459-plasmid-map-sequence-id-367469

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  5. phCMV-229E-D15

    Plasmid
    #188908
    Purpose
    express truncated 229E spike for pseudovirus production
    Depositor
    David Nemazee
    Insert
    229E spike (S 229E)
    Expression
    Mammalian
    Mutation
    c-terminal 15aa deletion
    Promoter
    CMV
    Available Since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    188908-plasmid-map-sequence-id-370665

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  7. SPYtag-GFPnanobody

    Plasmid
    #214837
    Purpose
    This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.
    Insert
    SPYtag-GFPnanobody
    Tags
    His-TEV-SUMO-
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214837-plasmid-map-sequence-id-425310

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  9. TRUPATH Triple Gai2

    Plasmid
    #196049
    Purpose
    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196049-plasmid-map-sequence-id-385863

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  10. pCDF-Frb-v1

    Plasmid
    #201923
    Purpose
    E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.
    Depositor
    Richard Cooley, Ryan Mehl
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    FrbA
    FrbB
    FrbC
    FrbD
    FrbE
    Expression
    Bacterial
    Promoter
    T7 (modified), T7 (modified, moderate), T7 (modif…
    Available Since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201923-plasmid-map-sequence-id-395412

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  13. pCAG-N2cN

    Plasmid
    #100801
    Purpose
    express rabies virus CVS N2c strain nucleoprotein gene (N2cN)
    Depositor
    Ian Wickersham
    Insert
    rabies virus CVS N2c strain nucleoprotein gene
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    100801-plasmid-map-sequence-id-189511

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  18. pPD292 PPH/HBB_IVS2/-T2A-dCas9-NFZ-P2A-BFP

    Plasmid
    #249156
    Purpose
    Overexpression of PPH-T2A-dCas9-NFZ-P2A-BFP with HBB IVS2 intron in PPH coding sequence and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    PPH/HBB_IVS2/-T2A-dCas9-NFZ-P2A-mTagBFP2 (HBB Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249156-plasmid-map-sequence-id-496442

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  30. Zika NS5 RdRp

    Plasmid
    #204788
    Purpose
    Bacterial expression of Zika NS5 RdRp
    Depositor
    ASAP Discovery, Frank von Delft
    Insert
    Zika NS5 RdRp domain
    Expression
    Bacterial
    Available Since
    April 25, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    204788-plasmid-map-sequence-id-417058

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Showing: 10741 - 10770 of 173901 results