161,152 results

1. pCMVtight-UPRT-T2A-RFP-IRES-CD

   Plasmid

   #126677

   Purpose

   Doxycycline inducible expression of UPRT and CD along with reporter RFP

   Depositor

   Joan Massague

   Article

   Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

   Insert

   UPRT-T2A-RFP-IRES-CD (FCY1 Toxoplasma gondii, Budding Yeast)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   tight TRE

   Available since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-cfGFP

   Plasmid

   #200773

   Purpose

   Expresses cysteine-free GFP (cfGFP) under synapsin promoter

   Depositor

   Alexander Dityatev

   Article

   Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

   Insert

   cysteine-free GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. phASCL1-N106

   Plasmid

   #31781

   Depositor

   Jerry Crabtree

   Article

   Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

   Insert

   achaete-scute complex homolog 1 (ASCL1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mTagBFP2-Endosomes-14

   Plasmid

   #55293

   Purpose

   Localization: Endosomes, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   Endosomes (RHOB Human)
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pEvol-NnSULT1C1-cysDNCQ

   Plasmid

   #188983

   Purpose

   biosynthesize sulfotyrosine in E. coli.

   Depositor

   Han Xiao

   Article

   Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

   Inserts

   NnSULT1C1
   cysD
   cysN
   cysC
   cysQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

   Plasmid

   #74010

   Purpose

   Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

   Depositor

   Filippo Del Bene

   Article

   Di Donato et al Genome Res. 2016 Mar 8.

   Inserts

   5xUAS
   Cas9
   Cre
   U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMVHA SUZ12

   Plasmid

   #24232

   Purpose

   Mammalian expression of human SUZ12 with HA tag

   Depositor

   Kristian Helin

   Article

   Pasini et al EMBO J. 2004 Oct 13. 23(20):4061-71.

   Insert

   SUZ12 (SUZ12 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET11phiC31poly(A)

   Plasmid

   #18942

   Depositor

   Michele Calos

   Article

   Groth et al Genetics. 2004 Apr . 166(4):1775-82.

   Insert

   phiC31 integrase (int Streptomyces phage phiC31)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-lyso-pHoenix

   Plasmid

   #70112

   Purpose

   Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

   Depositor

   Christian Rosenmund

   Article

   Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

   Insert

   lyso-pHoenix (Cd63 Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat, Mouse)
   

   Use

   Tags

   Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDMyc-TNPO1

    Plasmid

    #86081

    Purpose

    A mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Transportin-1 (TNPO1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-TPH2-Cre

    Plasmid

    #189616

    Purpose

    Expresses Cre recombinase under the control of the tryptophan hydroxylase promoter

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    Cre Recombinase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tph2

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-Sp1

    Plasmid

    #12097

    Depositor

    Robert Tjian

    Insert

    Sp1 (SP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa1-87 deleted

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCherry.90 beta

    Plasmid

    #108223

    Purpose

    Mammalian expression vector for mCherry fusion to human Hsp90 beta

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    mCherry-Hsp90b (HSP90AB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNDGG003

    Plasmid

    #231316

    Purpose

    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Tc-par-ELT4; GG assembly from pOGG010, pOGG012, pOGG014, pOGG042, and pOGG004, TcR

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223-SYK

    Plasmid

    #23907

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    SYK (SYK Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRX-IB-HaloTag7-mGFP-KDEL

    Plasmid

    #184904

    Purpose

    Stable expression of HaloTag7-mGFP-KDEL in mammalian cells to measure ER-phagy activity

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    KDEL
    

    Use

    Tags

    HaloTag7 and monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmTurquoise2-T2A-Venus(L68V)

    Plasmid

    #60494

    Purpose

    Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    mTurquoise2-T2A-mVenus(L68V)
    

    Use

    Tags

    T2A peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTXB1-nbOcIgG-Tn5

    Plasmid

    #184285

    Purpose

    Anti-rabbit IgG Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

    Depositor

    New York Genome Center, Ivan Raimondi

    Article

    Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

    Insert

    nbOcIgG-Tn5
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG25

    Plasmid

    #35121

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    natMX4
    

    Use

    Yeast replicating

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW57-MCS1-P2A-MCS2 (RFP)

    Plasmid

    #80923

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET30b-7+-SrtA

    Plasmid

    #105602

    Purpose

    expressed 7+ sortase A variant in E.coli

    Depositor

    Stephanie Dougan

    Article

    Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

    Insert

    S.aureus Sortase A, 7+ mutant
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3β-FLAG-CBP-HA

    Plasmid

    #32908

    Depositor

    Tso-Pang Yao

    Article

    Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

    Plasmid

    #86828

    Purpose

    Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    LINC00673 (LINC00673 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHR-SIN-BX SpyCatcher003-hCD52

    Plasmid

    #210565

    Purpose

    Expresses Spycatcher003 on the cell surface using human CD52 hinge

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    Spycatcher003 fused to human CD52 hinge
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. myc-mTOR

    Plasmid

    #1861

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    mTOR (Mtor Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-N1 hMOG-beta1-EGFP

    Plasmid

    #160978

    Purpose

    Expresses a human myelin oligodendrocyte glycoprotein isoform beta1 - EGFP fusion protein in mammalian cells

    Depositor

    Markus Reindl

    Article

    Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

    Insert

    Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta1 (MOG Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mEmerald-ARP2-C-14

    Plasmid

    #53992

    Purpose

    Localization: Actin Related Protein, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    ARP2 (ACTR2 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

28. Gamillus/ pcDNA3

    Plasmid

    #124837

    Purpose

    mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

    Depositor

    Takeharu Nagai

    Article

    Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

    Insert

    Gamillus
    

    Use

    Tags

    No tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-nTermPuroR-nTermIntein

    Plasmid

    #208378

    Purpose

    Episomal plasmid - for expression of 2 proteins linked with EGFP and n-terminal split puromycin resistance and split intein

    Depositor

    Daniel Hawiger

    Article

    Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human CMV

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-protein1-IRES-protein2-IRESatt-cTermIntein-cTermPuroR

    Plasmid

    #208379

    Purpose

    Episomal plasmid - for expression of 2 proteins linked with c-terminal split intein and split puromycin resistance

    Depositor

    Daniel Hawiger

    Article

    Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human CMV

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge