We narrowed to 8,688 results for: App

1. PB09-TRE-beta-globin-miR-ctrl-EF1a-GFP

   Plasmid

   #117319

   Purpose

   Overexpression of miR-124-surrounding locus (not miR-124) in eurkaryotic cells, encoded in a human beta-globin intron

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   hsa-miR-CTRL
   

   Use

   Transposon-mediated integration

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   miR-124 deleted

   Promoter

   Tet-inducible promoter

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-DDX11-Flag-KAK

   Plasmid

   #120729

   Purpose

   Expression of a Flag-tagged version of DDX11 KAK mutant in mammalian cells

   Depositor

   Francesca Pisani

   Article

   Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

   Insert

   DDX11 KAK mutant (DDX11 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Substitution of E201, Y202 and E203 with K, A and…

   Promoter

   CMV

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTWIN1-His6-Ssp-Httex1-23Q-A2C-A60C

   Plasmid

   #114613

   Purpose

   Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 23 polyQ repeat

   Depositor

   Hilal Lashuel

   Article

   Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

   Insert

   Huntingtin Exon1 (HTT Human)
   

   Use

   Tags

   His6-Ssp DnaB intein

   Expression

   Bacterial

   Mutation

   A2C, A60C; 23 polyQ repeat

   Promoter

   Available since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223 SARS-CoV2 TEV-ORF7B

   Plasmid

   #154422

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of o…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pDONR223 SARS-CoV2 TEV-ORF6

   Plasmid

   #154420

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF6 (ORF6 SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of o…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   July 7, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pDONR223 SARS-CoV2 TEV-ORF7A

   Plasmid

   #154421

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of o…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   July 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pDONR223 SARS-CoV2 TEV-ORF8

   Plasmid

   #154423

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF8 (ORF8 SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of o…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   July 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pDONR223 SARS-CoV2 TEV-NSP9

   Plasmid

   #154408

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP9 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of n…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   July 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pDONR223 SARS-CoV2 TEV-NSP15

   Plasmid

   #154413

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP15 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of n…

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   July 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pKK-RNAi-nucCHERRYmiR-TEV-FLAG

    Plasmid

    #105813

    Purpose

    Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    RNAi; Flp-in competent

    Tags

    TEV-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223-ZNF698

    Plasmid

    #88666

    Purpose

    Donor Vector containing ZNF698 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF698 (ZFP57 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MSCV-NSD3-short del(280-342)

    Plasmid

    #72550

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 280-342 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    280-342 aa deletion

    Promoter

    LTR

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTWIN1-His6-Ssp-Htt2-90-43Q-P90C

    Plasmid

    #114626

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 43 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    P90C; 43 polyQ repeat

    Promoter

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTWIN1-His6-Ssp-Htt2-90-15Q-P90C

    Plasmid

    #114611

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 15 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Use

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    P90C; 23 polyQ repeat

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221-HES5

    Plasmid

    #101409

    Purpose

    Donor Vector containing HES5 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    HES5 (HES5 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LCV2_AAVS1_sgRNA_3

    Plasmid

    #155089

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSCV-NSD3-short del(384-645)

    Plasmid

    #72549

    Purpose

    expresses 3*FLAG tagged human NSD3-short with 384-645 aa deletion

    Depositor

    Christopher Vakoc

    Article

    Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

    Insert

    NSD3-short (NSD3 Human)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    384-645 aa deletion

    Promoter

    LTR

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX

    Plasmid

    #112762

    Purpose

    express GFP-4EBP1-Rheb15(CAAX mutation)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Rheb15 (CAAX mutant) (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Rheb15(CAAX mutant)

    Expression

    Mutation

    CAAX mutation to prevent lipid localization

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR207 SARS-CoV-2 E 27nt-del_nostop

    Plasmid

    #153955

    Purpose

    Gateway-compatible Entry vector, with insert of E CDS bearing a 27nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    E (E SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    June 23, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pDONR223 SARS-CoV2 TEV-NSP8

    Plasmid

    #154407

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP8 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    July 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge