Addgene

We narrowed to 4,777 results for: Mos

Showing: 1401 - 1440 of 4777 results

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pX330.LoxP

Plasmid

#66586

Purpose

sgRNA for LSL

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LoxP sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
pET28a(+)/FbGH30

Plasmid

#86463

Purpose

Expression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coli

Depositor

Jan-Hendrik Hehemann

Article

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FbGH30

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a(+)/FaGH17A

Plasmid

#86462

Purpose

Expression of the Beta-1,3-Endo-Laminarinase FaGH17A in E.coli

Depositor

Jan-Hendrik Hehemann

Article

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FaGH17A

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pX330.iGFP2

Plasmid

#66582

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pFLLFLN

Plasmid

#40030

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

FRT-Lox5171-Lox2272-FRT-loxP
Neo

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

None and SV40

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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BSP179

Plasmid

#110915

Purpose

For cloning expression cassettes by HR in yeast, Universal MosSCI compatible

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Type

Empty backbone

Use

Synthetic Biology; Yeast homologous recombination…

Tags

Expression

Worm

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pX330.iGFP5

Plasmid

#66584

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
pX330.LoxPO

Plasmid

#66585

Purpose

sgRNA for LSL

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LSL-Tomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pTlpAsp-Wasabi

Plasmid

#86113

Purpose

mWasabi reporter gene driven by TlpA promoter with scrambled Pribnow box

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Insert

mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

pTlpA (scrambled Pribnow box)

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pX330.iGFP3

Plasmid

#66583

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only
pXD80EN13-Elen0616-Cber(ElDSM2243)

Plasmid

#225308

Purpose

Expresses Eggerthella lenta DSM 2243 Cber in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Cber

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGGC-PEA3AT

Plasmid

#210536

Purpose

Cloning P. sativum PEA3A terminator as GreenGate module C

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

PEA3At

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCxcl1-Firefly-1-Tbx5-site

Plasmid

#177832

Purpose

Luciferase reporter for mouse Cxcl1 with one Tbx5 consensus binding site

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Cxcl1

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCxcl1-Firefly-2-Tbx5-sites

Plasmid

#177833

Purpose

Luciferase reporter for mouse Cxcl1 with two Tbx5 consensus binding sites

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Cxcl1

Available since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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F5SJF3

Plasmid

#163233

Purpose

Inducible expression of UniProt identifier F5SJF3

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

F5SJF3

Use

Tags

10xHis and T7

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSLfa-PUb-EGFP-3'UTR#13

Plasmid

#176338

Purpose

The synthetic 3'UTR#13 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoter

Depositor

Zach Adelman

Article

Chae et al RNA Biol. 2021 Aug 31:1-9. doi: 10.1080/

Insert

3'UTR#13

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pIRENE-div-Firefly

Plasmid

#170661

Purpose

Luciferase reporter for mouse IRENE-div lncRNA

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

IRENE-div

Use

Luciferase

Tags

Expression

Mutation

Promoter

IRENE-div

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCxcl1-Firefly-Chantico-Tdel

Plasmid

#163900

Purpose

Luciferase reporter for mouse Cxcl1 with Chantico promoter Tbx5 site deletion

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Cxcl1

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pChantico-Tdel-Firefly

Plasmid

#163902

Purpose

Luciferase reporter for Chantico lncRNA with Tbx5 site deletion

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Chantico with Tbx5 consensus binding site deletion

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pChantico-pAS-Firefly

Plasmid

#163903

Purpose

Luciferase reporter for Chantico lncRNA with triple pAS insertion downstream of the promoter

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

Chantico

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pUC57-Ter

Plasmid

#170634

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

Mini-Tn6677, Ter cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pFd022.R2

Plasmid

#137967

Purpose

Expresses Prochlorococcus phage P-SSM2 phage Fd

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus phage P-SSM2 ferredoxin

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd029.R2

Plasmid

#137968

Purpose

Expresses Prochlorococcus marinus NATL1A Fd 1

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus NATL1A ferredoxin 1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd030.R2

Plasmid

#137969

Purpose

Expresses Prochlorococcus marinus NATL2A Fd 1

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus NATL2A ferredoxin 1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJTA022

Plasmid

#137975

Purpose

Inucible expression of Prochlorococcus phage P-SSM2 phage Fd for purification

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus phage P-SSM2 ferredoxin

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSG73-β1ARts-sfGFP

Plasmid

#102459

Purpose

Expresses a fusion protein of thermostabilized β1 adrenergic receptor and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.

Depositor

Richard Murray

Article

Guo et al bioRxiv 104455

Insert

β1AR

Use

Tags

His tag and sfGFP

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pFLN

Plasmid

#40029

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

FRT-loxP
Neo

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

None and SV40

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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USP25_pCSdest

Plasmid

#53817

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

USP25 (USP25 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
35S:RUBY

Plasmid

#160908

Purpose

RUBY under the control of CaMV 35S promoter/marker for transformation

Depositor

Yunde Zhao

Article

He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

Insert

RUBY and CaMV 35S promoter

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA Scramble

Plasmid

#162011

Purpose

Lentiviral negative control vector containing scramble shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

scramble shRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLacO-ISce1

Plasmid

#58505

Purpose

provides 256 Lac operator repeats adjacent to the ISce1 restriction site

Depositor

Tom Misteli

Article

Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

Insert

Lac repeats adjacent to 6 copies of the ISce1 restriction site

Use

Tags

Expression

Bacterial

Mutation

Promoter

no promoter

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-TO-dCas9-3XmCherry

Plasmid

#64108

Purpose

Expression of Sp dCas9-3XmCherry in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD-Flp

Plasmid

#122969

Purpose

Expresses flp recombinase in bacteria upon arabinose induction. No flp activity is detected without induction.

Depositor

Lydia Freddolino

Article

Scholz et al Cell Syst. 2019 Mar 27;8(3):212-225.e9.

Insert

Flp recombinase

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Aug. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pHAGE-TO-dCas9-3XGFP

Plasmid

#64107

Purpose

Expression of Sp dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Mutation

Promoter

CMV-TO

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
OA-1067B

Plasmid

#200252

Purpose

pBac-U6-gRNA(doublesex)-3xp3-tdTomato

Depositor

Omar Akbari

Article

Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

Insert

4 gRNAs targeting Doublesex

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKI015

Plasmid

#11570

Depositor

Detlef Weigel

Article

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Activation tagging

Tags

Expression

Plant

Mutation

Promoter

Available since

May 4, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pTlpA36-Wasabi

Plasmid

#86115

Purpose

mWasabi reporter gene gated by TlpA36

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA36
mWasabi

Use

Tags

6xHis

Expression

Bacterial

Mutation

D341A, P60L, D135V, K187R, K202I, L208Q

Promoter

pTlpA and pTlpA, LacI

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSKI074

Plasmid

#11571

Depositor

Detlef Weigel

Article

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Activation tagging

Tags

Expression

Plant

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-TID-HA3-HIS3MX6

Plasmid

#227981

Purpose

To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID

Use

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
NLS-Halo

Plasmid

#200197

Purpose

Expression for SV40 NLS

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

3X SV40 NLS-Halo

Use

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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