We narrowed to 18,759 results for: nls
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Plasmid#113038PurposeAAV vector for expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)DepositorInsertSV40 NLS-AcrIIA4
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJM592 EF1α 3xFLAG-NLS-DsDed-ZF10-intN-VP64 in TUPV2
Plasmid#161568PurposeConstitutive expression of 3xFLAG-NLS-DsDed-ZF10-intN-VP64 under the EF1α promoterDepositorInsert3xFLAG-NLS-DsDed-ZF10-intN-VP64
UseSynthetic BiologyTags3x-FLAGExpressionMammalianPromoterEF1αAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
TwinStrep-SUMO-NLS-BhCas12b_v4
Plasmid#158615PurposeFor protein expression of BhCas12b_v4DepositorInsertNLS-BhCas12b_v4
Tags6xHIS-TwinStrep-SUMOExpressionBacterialAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
AiP1181-Ai224 (TICL-nls-EGFP-ICF-nls-dTomato)
Plasmid#181760Purposetargeting vectorDepositorInsertnls-tagged EGFP and nls-tagged dTomato
ExpressionMammalianAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-BFP-NLS-P2A-RSV P-1xSunTag@72AA
Plasmid#246863PurposeExpress BFP-NLS-P2A-RSV P-1xSunTag@72AA (Pexo-SunTag) for RSV imaging.DepositorInsertBFP-NLS-RSV P-1xSunTag@72AA
UseLentiviralTagsBFPExpressionMammalianMutationInserted a single SunTag copy at amino acid 72 of…PromoterSFFVAvailable sinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag-NLS-Smad2
Plasmid#14931DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-Smad2 (SMAD2 Human)
TagsFlagExpressionMammalianMutationfull-length Smad2 is fused to a nuclear-localizat…Available sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
FRP2371
Plasmid#127577PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, His3MX selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorExpressionYeastAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pST1374-scFv-APOBEC-UGI-GB1
Plasmid#113029PurposeExpresses scFv-rAPOBEC1-UGI in mammalian cellsDepositorInsertscFv-rAPOBEC1-UGI-GB1
ExpressionMammalianPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFTK056
Plasmid#171328PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertSpCas9-NLS
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
BII-STAR-tdT
Plasmid#133396PurposePiggybac vector for ASCL2 reporterDepositorInserttdTomato-NLS
ExpressionMammalianMutationN/APromoterStem Cell Specific ASCL2 response elements with L…Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtinGW
Plasmid#133377PurposePiggybac vector for constitutive expressionDepositorTypeEmpty backboneTagsFlagExpressionMammalianPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAcFRS.2.t1
Plasmid#73544PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAcFRS.2.t1
pAcFRS.2.t1
ExpressionBacterialAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRO280
Plasmid#233846PurposeMoClo-YTK; ConL1/R2 cassette; ABA systems; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1DepositorInsertpTDH3-NLS-VP48-2xOaf1-ABI-tENO1
ExpressionYeastAvailable sinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWN059
Plasmid#233853PurposeMoClo-YTK; ConL1/R2 cassette; Auxin systems; pTDH3-NLS-VP48-2XOaf1-AIDΔ34-tENO1DepositorInsertpTDH3-NLS-VP48-2XOaf1-AIDΔ34-tENO1
ExpressionYeastAvailable sinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWN061
Plasmid#233854PurposeMoClo-YTK; ConL1/R2 cassette; NS3 systems; pTDH3-NLS-VP48-2XOaf1-DNCR2-tENO1DepositorInsertpTDH3-NLS-VP48-2XOaf1-DNCR2-tENO1
ExpressionYeastAvailable sinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEX.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
Plasmid#45565PurposeExpresses I-Sce I nuclease in mammalian cells and BFP as a trackerDepositorPublicationInsertsI-Sce I Nuclease
BFP
TagsHAExpressionMammalianAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP
Plasmid#45574PurposeExpresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backboneDepositorPublicationInsertco I-Sce I
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2
Plasmid#219548PurposeNLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
ExpressionMammalianMutationThe (SAGG)x4 linker between GAL4(1-65) and the PC…PromoterCMVd1Available sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRD406
Plasmid#163111PurposeExpression of mNeonGreen_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS_mNeonGreen_H-NSdbd_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD398
Plasmid#163105PurposeExpression of mCherry_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS_mCherry_H-NSdbd_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD399
Plasmid#163106PurposeExpression of mEGFP_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS_mEGFP_H-NSdbd_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-wtSpCas9 (without sgRNA)
Plasmid#92353PurposeExpression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xFlag-NLS-wild-type SpCas9-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianPromoterCbhAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
phage - ubc - nls - mcp - egfp - BirA*
Plasmid#131134PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-MCP-EGFP-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBUN421
Plasmid#62204PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoterTaU3 and UbiAvailable sinceFeb. 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-c-Myc NLS-FKBP-Venus N-T2A-mRFP
Plasmid#100979PurposeEncodes half of a protein delivery sensor ( NLS-FKBP-VN-T2A-RFP) for expression in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertc-Myc NLS-FKBP-Venus N-T2A-mRFP
ExpressionMammalianPromoterCAGAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMP416
Plasmid#134996PurposedCas9-m6A Writer. Methylates adenines at local GATC sites. pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
UseSynthetic BiologyTags3xFLAG, E coli Dam(N132A), and dCas9ExpressionMammalianAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-HyPerFLEX-NLSx3-WPRE
Plasmid#217563PurposeMammalian expression of HyPerFLEX targeted to the nucleusDepositorInsertHyPerFLEX-NLS
UseAAVExpressionMammalianPromoterCAGAvailable sinceOct. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME nls BFP FLAG WPRE bGH pA (JDW 484)
Plasmid#224539PurposeA Gateway compatible middle entry clone containing a 3x-NLS-mTagBFP2-FLAG for labeling the nucleusDepositorInsertnls-mTagBFP2-FLAG_WPRE-bGH-pA
UseGateway CloningAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a_eeBxb1_Int_2x_NPL_c-term_NLS_pTwist
Plasmid#244901PurposeExpression of eeBxb1DepositorInserteeBxb1
ExpressionMammalianPromoterEF1aAvailable sinceNov. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
Plasmid#45564PurposeExpresses I-Sce I nuclease and BFP tracker in mammalian cells, codes for eGFP donor sequenceDepositorPublicationInsertsd14 eGFP donor
I-Sce I Nuclease
BFP
TagsHAExpressionMammalianMutationdeleted n' 15 amino acidsAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMEF2c-AHF-Dre-nls (JDW 35)
Plasmid#242585PurposeThe Mef2c anterior heart field promoter driving expression of Dre recombinase.DepositorInsertDRE-nls
ExpressionMammalianPromotermouse Mef2cAvailable sinceApril 8, 2026AvailabilityAcademic Institutions and Nonprofits only -
YCe1903 HC_Kan_SV40-NLS_p6
Plasmid#100684Purposepart designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targetingDepositorInsertSV40-NLS
UseSynthetic BiologyAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTSK450
Plasmid#34552DepositorInsertMS2-CP-NLS
TagsmCherryExpressionYeastPromoterMet25Available sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - mCherry - BirA*
Plasmid#131136PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-mCherry-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP
Plasmid#32627DepositorInsertDonor + Sce BFP
UseLentiviralAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA
Plasmid#48253PurposeLITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators.DepositorInsertCRY2PHR-NLS-VP64
UseAAV; TaleTags2A_GFPExpressionMammalianPromoterEF1-alphaAvailable sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT2-7xTcf-NLS-YNL
Plasmid#65711PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-YNL)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert7xTcf, minCMV, 3xNLS, YNL
UseLuciferase; Tol2 systemAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRFP-NLS
Plasmid#105296PurposeTo visualize nuclear dynamics in living cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRac1 (RAC1 Human)
TagsRFPExpressionMammalianMutationaa encoding 172-192Available sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTSK368
Plasmid#34551DepositorInsertMS2-CP-NLS
TagsGFPExpressionYeastPromoterMet25Available sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSin_EF1a_ERT2_CreN59_nMag_P2A_mCh_IRES_PuroR
Plasmid#192636PurposeExpresses ERT2-CreN59-nMag-NLS with mCherry marker.DepositorInsertERT2_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterEF1alphaAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only