-
Plasmid#115912Purposedonor to insert IRES-dTomato at the GAPDH locus in human cellsDepositorInsertIRES-dTomato
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHA853 - mec-4p::Fynempty
Plasmid#139210PurposeControl vector for Fyn kinase expression in mec-4 neuronsDepositorInsertFyn (FYN Human)
UseTagsExpressionWormMutationdeletion and frame shift to prevent Fyn expressionPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHA851 - osm-10p::Fynempty
Plasmid#139208PurposeControl vector for Fyn kinase expression in osm-10 neuronsDepositorInsertFyn (FYN Human)
UseTagsExpressionWormMutationdeletion and frame shift to prevent Fyn expressionPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRepair-mTq2-CTNNB1
Plasmid#153433PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsmTurquoise2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6-FnCas12a-TLR-MCV1-gRNA
Plasmid#117412PurposeFnCas12a-gRNA targeting TLR2.0DepositorInsertFnCas12a gRNA targeting TLR 2.0
UseTagsExpressionMutationPromoterU6Available sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
289aa ELL2
Plasmid#127266PurposeFor in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.DepositorInsertNH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
UseIn vitro translationTagsExpressionMutationThree Mets (133, 138, 186) to Ileu. Contains 289…PromoterT7Available sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Xba ELL2 Met133, 138, 186 to Ileu
Plasmid#127269PurposeFor in vitro translation of ELL2 with with internal HA tag and Met133, 138, 186 IleuDepositorInsertELL2 Met133, 138, 186 to Ileu (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet133, 138, 186 to Ileu HA tag after M186PromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA tagged Sph ELL2 Met133, 138, 186 to Ileu
Plasmid#127271PurposeFor in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th MetDepositorInsertELL2 Met133, 138, 186 to Ileu (ELL2 Human)
UseIn vitro translationTagsExpressionMutationMet133, 138, 186 to Ileu with HA tag after 8th MetPromoterT7Available sinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
frameshift ELL2
Plasmid#127257PurposeFor in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.DepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationXhoI site between the 1st &2nd Met was filled…PromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
564aa ELL2 bluescript
Plasmid#127267PurposeFor in vitro translation of human ELL2 lacking COOH regionDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationAddgene Plasmid 127256--SphI site filled in, then…PromoterT7Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)
Plasmid#122450PurposeExpression of inositol 1,4,5-trisphosphate 3-kinase ADepositorInsertinositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
UseTagsbeta-galactosidaseExpressionBacterialMutationcoding sequence from V66 till stop codonPromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_CreNLS_PP7
Plasmid#232439Purposeexpression plasmid to produce Cre-NLS mRNA with a PP7 aptamer in the 3' UTRDepositorInsertCre–MYC-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_miniENVLPE
Plasmid#232431PurposeExpression plasmid to produce miniENVLPE particlesDepositorInsertminiENVLPE
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_mGL_PP7
Plasmid#232440Purposeexpression plasmid to produce mGreenLantern mRNA with a PP7 aptamer in the 3' UTRDepositorInsertmGreenLantern
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
PEmax_AAVS1_knockin_HDR_donor
Plasmid#223000PurposeHDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPEmax
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-BsmbI-LoxP-Ef1a-EM7-mCherry-Neo-PolyA-LoxP
Plasmid#192822PurposeGolden Gate compatible plasmid with mCherry and NeoDepositorInsertmCherry and Neo
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
mAID-BRD4 donor
Plasmid#140650PurposeBRD4 tagging with mAIDDepositorInsertBRD4 (BRD4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
CTCF-mAC donor (Hygro)
Plasmid#140646PurposeCTCF tagging with mAID-cloverDepositorInsertCTCF (CTCF Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
CTCF-mAC donor (Neo)
Plasmid#140645PurposeCTCF tagging with mAID-CloverDepositorInsertCTCF (CTCF Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-eTLR
Plasmid#214737Purposeencodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locusDepositorInsertAAVS1-eTLR (PPP1R12C Human, Chicken, Rabbit, CMV, Anguilla japonica, influenza, Synthetic, Bovine)
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-I-Sce1-eno2:Tau-IRES-egfp
Plasmid#112205PurposeUsed to make transgenic zebrafish expressing human 4R/0N Tau under the neuronal eno2 promoterDepositorInsertMAPT (MAPT Human)
UseZebrafish expressionTagseno2 exon 2 translational fusion, ires-egfp at c …ExpressionMutationPromotereno2 from zebrafishAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-SEC61B-mEGFP
Plasmid#115910Purposedonor to insert EGFP at the SEC61B locus in human cellsDepositorInsertSEC61B-mEGFP (SEC61B Human)
UseDonor templateTagsEGFPExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMB71
Plasmid#73085PurposeExpresses C. elegans optimized BirA under control of the intestinal elt-2 promoterDepositorInsertBirA
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
iMb-Control-Mosaic (IR98.10)
Plasmid#99748PurposeRosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different fluorescent and marker proteins localized to the membraneDepositorInsertMbYFP, MbTomato, MbKate2
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMB73
Plasmid#73086PurposeExpresses C. elegans optimized BirA under control of the seam and hypodermis wrt-2 promoterDepositorInsertBirA
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)
Plasmid#36881DepositorInsertsGFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMB37
Plasmid#73084PurposeExpresses C. elegans optimized BirA under control of the ubiquitous rps-27 promoterDepositorInsertBirA
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-TOMM20-mEGFP
Plasmid#115911Purposedonor to insert EGFP at the TOMM20 locus in human cellsDepositorInsertTOMM20-mEGFP (TOMM20 Human)
UseDonor templateTagsEGFPExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Drd1a->M71-IRES-tauGFP ACNF TV
Plasmid#105073PurposeTargeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-447 of Drd1a and an IRES-tauGFP followed by ACNF cassetteDepositorUseMouse TargetingTagsExpressionMutationDrd1a coding sequence followed by IRES-tauGFP ACNFPromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF TV
Plasmid#105196Purposetargeting vector to generate a Gucy1b2-IRES-nlsCre-IRES-taulacZ mouse strain.DepositorInsertGucy1b2-IRES-nlsCre-IRES-taulacZ-FNF (Gucy1b2 Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-SEC61B-dTomato
Plasmid#115913Purposedonor to insert dTomato at the SEC61B locus in human cellsDepositorInsertSEC61B-dTomato (SEC61B Human)
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-f1(+)-TOMM20-dTomato
Plasmid#115914Purposedonor to insert dTomato at the TOMM20 locus in human cellsDepositorInsertTOMM20-dTomato (TOMM20 Human)
UseDonor templateTagsdTomatoExpressionMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Cas9–superNLS
Plasmid#232430PurposeExpression plasmid for the SpCas9 nuclease with superNLSDepositorInsertCas9–superNLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_iPE-C_P2A_Csy4
Plasmid#232428PurposeExpression plasmid for iPE-C prime editor RNPs with additional Csy4 protectionDepositorInsertiPE-C–P2A–Csy4
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_ABE8e–superNLS
Plasmid#232429PurposeExpression plasmid for the ABE8e base editor with superNLSDepositorInsertABE8e–superNLS
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
PE7_AAVS1_knockin_HDR_donor
Plasmid#223001PurposeHDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPE7
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT_5'-xrRNA_NLuc_3'-XAP1
Plasmid#197264PurposeCloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' XAP1). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.DepositorTypeEmpty backboneUseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR
Plasmid#197265PurposeCloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.DepositorTypeEmpty backboneUseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
iChr2-Control-Mosaic (SO274)
Plasmid#99751PurposeSecond generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteinsDepositorInsertHIs-H2B-Cherry, H2B-EGFP, HA-H2B-Cerulean
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
iMb2-Control-Mosaic (SO273)
Plasmid#99750PurposeSecond generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteinsDepositorInsertMb2YFP-2A, Mb2Tomato-2A, Mb2-HA-Tfp1
UseCre/Lox and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only