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Plasmid#112943PurposeProduces a fusion between LSS-GFP2 and mCherry. It can be used as a positive control for FRETDepositorInsertLSS-GFP2 mCherry fusion
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfb12567
Plasmid#219927PurposePrCYC with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter CYC
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfb12566
Plasmid#219926PurposePrDGA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter DGA
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfb12622
Plasmid#219929PurposePrACT with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter ACT
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb12562
Plasmid#219923PurposePrGPD with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter GPD
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13186
Plasmid#219924PurposePrTEF with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter TEF
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb12620
Plasmid#219928PurposePrTPI with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter TPI
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb12561
Plasmid#219922PurposePrPGM with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter PGM
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb12565
Plasmid#219925PurposePrFBA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALIDepositorInsertPromoter FBA
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSU1/t35s[TMV]:MCS:NosT
Plasmid#212158PurposeThis binary vector has an empty backbone where you can insert a gene into a cassette with a strong promoter (truncated 35sCAMV) and UTR (Tomato Mosaic Virus)DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJEP304-pAAV-EFS-dSaCas9-VP64-pA
Plasmid#113679PurposeA EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted regionDepositorInsertde-catalyzed SaCas9
UseAAV, CRISPR, and Synthetic BiologyTagsNLS and VP64ExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - HA-KLF4 FL
Plasmid#34593DepositorInsertKLF4 (KLF4 Human)
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
VE-cad-eGFP Donor plasmid
Plasmid#92309PurposeKnock eGFP into human VE-cad locusDepositorInsertVEcad Homologous Recombination Arms (CDH5 Human)
UseCRISPRTagsExpressionMammalianMutationabout 30 a.a. truncated at end of proteinPromoterAvailable sinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
VE-cad KI gRNA1
Plasmid#92310PurposeCRISPR-GFP-gRNA for cutting VEcadDepositorInsertVEcad KI gRNA1 (CDH5 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
VE-cad KI gRNA2
Plasmid#92311PurposeCRISPR-GFP-gRNA for cutting VEcadDepositorInsertVEcad KI gRNA2 (CDH5 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc-C Med12His
Plasmid#49240Purposeexpresses human Med12 with His tag in insect cellsDepositorInsertMed12 (MED12 Human)
UseTagsHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-FoxO1_1R_10A_3D
Plasmid#106278PurposeFluorescent fusion protein for FoxO1DepositorInsertsUseTagsClover fluorescent proteinExpressionMammalianMutationDeleted amino acids 401-636, S209A, H212R, S215A,…PromoterEF1a/RPBSAAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D
Plasmid#110302PurposeExpresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1DepositorInsertp53-binding protein 1 (TP53BP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-Gnaq
Plasmid#170298PurposeEncoding mouse GnaqDepositorInsertA recombinant mouse Gnaq (Gnaq Mouse)
UseTagsN/AExpressionMammalianMutationAsilent mutation was introduced into the sgRNA-ta…PromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-Tet1 CD
Plasmid#70129PurposeExpress flag-tagged mouse Tet1 catalytic domain.DepositorInsertTen-Eleven Translocation 1 (Tet1 Mouse)
UseTagsflagExpressionMammalianMutationThe plasmid expresses TET1 catalytic domain.PromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only