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  1. pYUBcLIC-GFP

    Plasmid
    #183509
    Purpose
    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in mycobacteria.
    Depositor
    Martin Högbom
    Article
    Grave et al Protein Expr Purif. 2022 Oct;198:106132.
    Type
    Empty backbone
    Use
    Tags
    10x His-tag and folding reporter GFP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    183509-plasmid-map-sequence-id-362497

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  6. pNvT-MHC::mCh

    Plasmid
    #67943
    Purpose
    Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI
    Depositor
    Ulrich Technau
    Article
    Renfer et al Proc Natl Acad Sci U S A. 2010 Jan 5;107
    Insert
    mCherry
    Use
    Transgenic reporter in the sea anemone, nematoste…
    Tags
    Expression
    Mutation
    Promoter
    Myosin Heavy Chain1 (MyHC1)
    Available since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    67943-plasmid-map-sequence-id-266110

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  8. pHDR-USR-SpCas9

    Plasmid
    #208829
    Purpose
    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.
    Depositor
    Zhiying Zhang
    Article
    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    208829-plasmid-map-sequence-id-419661

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  11. SEP-Nanoluc-cyOFP1

    Plasmid
    #141082
    Purpose
    prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    SEP-Nanoluc-cyOFP1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Deletion of amino acids DISGG from positions 673 …
    Promoter
    EF1α
    Available since
    June 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    141082-plasmid-map-sequence-id-296810

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  14. Antares-SEPLuc C1A4E

    Plasmid
    #141088
    Purpose
    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc
    Depositor
    Jeak Ling Ding
    Article
    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41
    Insert
    Antares-T2A-puromycin-IRESv24-SEP-C1A4E
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Please see depositor comments
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    141088-plasmid-map-sequence-id-296867

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  15. pBeta-lacZ-attBx2

    Plasmid
    #124445
    Purpose
    Plasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.
    Depositor
    Yingguang Frank Chan
    Article
    Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554
    Type
    Empty backbone
    Use
    Enhancer reporter assay construct
    Tags
    LacZ
    Expression
    Mammalian
    Mutation
    Promoter
    beta globin minimal promoter
    Available since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    124445-plasmid-map-sequence-id-242276

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Showing: 701 - 720 of 9589 results