We narrowed to 62,913 results for: cloning

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pLenti-PGK-Neo-PIP-FUCCI

Plasmid

#118616

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Publication

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Promoter

hPGK

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pY108 (lenti-AsCpf1)

Plasmid

#84739

Purpose

Lenti virus delivery of AsCpf1 and crRNA guide

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
puromycin resistance gene

Use

Lentiviral

Tags

3xHA, NLS, and P2A

Promoter

EFS

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pBABEpuro-ERBB2

Plasmid

#40978

Depositor

Matthew Meyerson

Publication

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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gRNA_AAVS1-T1

Plasmid

#41817

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

Depositor

George Church

Publication

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T1

Use

CRISPR

Expression

Mammalian

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pET-6xHis-SUMO-MarathonRT

Plasmid

#109029

Purpose

Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

Depositor

Anna Pyle

Publication

Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

Insert

ERE_01490

Tags

6xHis-SUMO tag

Expression

Bacterial

Promoter

T7

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLenti-PGK-KRAS4B(G12V)

Plasmid

#35633

Depositor

Daniel Haber

Publication

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4B(G12V) (KRAS Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pDEST53-LRRK2-WT

Plasmid

#25044

Depositor

Mark Cookson

Publication

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pENTR4-FLAG (w210-2)

Plasmid

#17423

Purpose

Gateway entry vector for N-term FLAG tag

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

FLAG

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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dxCas(3.7)-VPR

Plasmid

#108383

Purpose

Mammalian expression vector for xCas9 3.7 with VP64-p65-Rta (VPR) fused to its C-terminus

Depositor

David Liu

Publication

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

dxCas(3.7)-VPR

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCRIS-PITChv2-dTAG-BSD (BRD4)

Plasmid

#91795

Purpose

PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.

Depositor

James Bradner, Behnam Nabet

Publication

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

FKBP_F36V-2xHA-P2A-BSD

Use

CRISPR

Expression

Mammalian

Mutation

Phenylalanine 36 to valine in FKBP12

Promoter

Promotorless

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCMV-PARP1-3xFlag-WT

Plasmid

#111575

Purpose

Expresses full length PARP1 in mammalian cells

Depositor

Thomas Muir

Publication

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pLenti-pHluorin_M153R-CD63-mScarlet

Plasmid

#172118

Purpose

Dual-color fluorescent reporter for CD63-positive exosome secretion and uptake

Depositor

Alissa Weaver

Publication

Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

Insert

CD63 (CD63 Human)

Use

Lentiviral

Tags

mScarlet and pHluorin_M153R in a small extracellu…

Expression

Mammalian

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pDEST14-SpyCatcher

Plasmid

#35044

Depositor

Mark Howarth

Publication

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyCatcher

Tags

His6

Expression

Bacterial

Mutation

Deletion of C-terminal till I113, and point mutat…

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pGP-CMV-jGCaMP8f

Plasmid

#162373

Purpose

Mammalian expression of ultrafast protein calcium sensor

Depositor

GENIE Project

Publication

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8f

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

CMV

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMH0001

Plasmid

#85969

Purpose

UCOE-SFFV-dCas9-BFP-KRAB

Depositor

Jonathan Weissman

Publication

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Insert

dCas9

Use

Lentiviral

Tags

BFP-KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

SFFV

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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BirA(R118G)-HA destination vector

Plasmid

#53581

Purpose

To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)

Depositor

Karl Kramer

Type

Empty backbone

Tags

BirA(R118G) and HA tag

Expression

Mammalian

Promoter

CMV

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCMV-15F11-HA-mEGFP

Plasmid

#129590

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Publication

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pCMV-PEmax-P2A-BSD

Plasmid

#174821

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-BSD

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDEST53-LRRK2-G2019S

Plasmid

#25045

Depositor

Mark Cookson

Publication

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Glycine 2019 mutated to Serine

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pENTR1A-GFP-N2 (FR1)

Plasmid

#19364

Purpose

Gateway entry vector for C-term eGFP fusion, frame N2

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced Green Fluorescent Protein

Use

Gateway Cloning

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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