We narrowed to 1,987 results for: endog

1. pCFJ150-Cas9(dpiRNA)

   Plasmid

   #107940

   Purpose

   eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

   Depositor

   Heng-Chi Lee

   Article

   Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

   Insert

   Cas9(dpiRNA)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   eft-3

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CibN-dCas9

   Plasmid

   #60551

   Purpose

   Expresses CibN-dCas9 in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   CibN-dCas9
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC57-Amp_CRISPIE donor B3

   Plasmid

   #172844

   Purpose

   CRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mTurquoise2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC57-Amp_CRISPIE donor B4

   Plasmid

   #172845

   Purpose

   CRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mNeonGreen
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-dCas9-CibN

   Plasmid

   #60552

   Purpose

   Expresses dCas9-CibN in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   dCas9-CibN
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMDC32B-AtMIR390a-NbSu-2

   Plasmid

   #213400

   Purpose

   Plant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursor

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:

   Insert

   Arabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   2x35S

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-PITCh-TUBB4B

   Plasmid

   #207785

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 Flag-MBD1 R44Q-14-mVenusC

   Plasmid

   #191394

   Purpose

   R44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenus

   Depositor

   Albert Jeltsch

   Article

   Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

   Insert

   pcDNA3 Flag-MBD1 R44Q-14-mVenusC
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R44Q

   Promoter

   CMV

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. S12.1_Empty_Bridge

   Plasmid

   #127670

   Purpose

   Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMC_mNG2(11)_BSDminus_F0

    Plasmid

    #184162

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mNG2(11)
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Psicheck2 Sox4 full length 3'UTR

    Plasmid

    #26989

    Depositor

    Joan Massague

    Article

    Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

    Insert

    Sox4 Full length 3' UTR (SOX4 Human)
    

    Use

    Luciferase

    Tags

    Renilla Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pMGS59(AID-ARF-P2A-Hygromycin)

    Plasmid

    #138174

    Purpose

    An AID-ARF-P2A-Hygromycin fusion cassette to tag the protein of interests with auxin-inducible degron and ARF at the c-terminal

    Depositor

    Michael Guertin

    Article

    Sathyan et al Curr Protoc Mol Biol. 2020 Sep;132(1):e1

    Insert

    AID-ARF-P2A-Hygromycin
    

    Use

    Tags

    AID-ARF clamp degron tag

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. S12.2_Bridge + Target

    Plasmid

    #127671

    Purpose

    Ampicillin resistant multiplexing backbone with CRY2HA and mCherry

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACUH-GFP1-10 mitochondrial matrix

    Plasmid

    #172063

    Purpose

    UAS vector to express GFP1-10 in the mitochondrial matrix

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP1-10 mitochondrial matrix
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Empty Anchor Control

    Plasmid

    #127665

    Purpose

    Encodes for puromycin resistance expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJM1-Rap2A-3xFLAG

    Plasmid

    #87973

    Purpose

    Expression of Rap2A in mammalian cells

    Depositor

    David Sabatini

    Article

    Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMC_mNG2(11)_BSDminus_F2

    Plasmid

    #184164

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mNG2(11)
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMC_mNG2(11)_BSDminus_F1

    Plasmid

    #184163

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mNG2(11) synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mNG2(11)
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. XW20

    Plasmid

    #65836

    Purpose

    Punc-4-QF-3'UTR-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed, and no SL2-mcherry)

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    OR74A quinic acid utilization activator (qa-1F)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    The endogenous SphI, KpnI and SacI sites in QF ar…

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pChlr-4SYR

    Plasmid

    #52158

    Purpose

    Encodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5C

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Insert

    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed 12N to 12S, 13H to 13Y, and 16Q to 16R in…

    Promoter

    Available since

    April 28, 2014

    Availability

    Academic Institutions and Nonprofits only