We narrowed to 55,043 results for: tra

1. pAAVS1_EF1a-MitoTrap-mCherry-IRES-Puro

   Plasmid

   #197067

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, constitutive expression of MitoTrap-mCherry

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   MitoTrap-mCherry
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA

   Plasmid

   #133339

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_ctrA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL03

   Plasmid

   #158793

   Purpose

   Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

   Insert

   Neuraminidase A/NL/109/03
   

   Tags

   TwinStrep_sfGFP_tetrabrachion

   Expression

   Mammalian

   Mutation

   AA61-469

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.23-IL2RA CaRE1 (plus strand)

   Plasmid

   #91837

   Purpose

   Luciferase reporter for IL2RA enhancer (IGI-P0614)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   IL2RA CaRE1 (plus strand) (IL2RA Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Perfect match to reference sequence

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pspCas9-3'UTR-Tetra-com-vector

   Plasmid

   #132553

   Purpose

   Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Inserts

   Cas9
   sgRNA scaffold with com replacing the Tetraloop
   

   Expression

   Mammalian

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol2-nbt-Electra1-P2A-mScarlet

   Plasmid

   #196113

   Purpose

   zebrafish expression of nbt-Electra1-P2A-mScarlet

   Depositor

   Reinhard Köster

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   nbt-Electra1-P2A-mScarlet
   

   Use

   Zebrafish expression

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. P-miR-145 (pTransluc-miR-145-1)

   Plasmid

   #21496

   Depositor

   Kenneth Kosik

   Article

   Xu et al Cell. 2009 May 15. 137(4):647-58.

   Insert

   1.5kb 5' of miR-145 (MIR145 Human)
   

   Use

   Luciferase

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA

   Plasmid

   #133342

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_ctrA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.23-CD69 CaRE3 (plus strand)

   Plasmid

   #91844

   Purpose

   Luciferase reporter for CD69 enhancer (IGI-P0621)

   Depositor

   Jacob Corn

   Article

   Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

   Insert

   CD69 CaRE3 (plus strand) (CD69 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Missing 1 base from 13 base polyT tract beginning…

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK-flag-GFP 1-10 tetra Cys

    Plasmid

    #78589

    Purpose

    Expression flag-GFP 1-10 tetra Cys in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-GFP 1-10 tetra Cys
    

    Tags

    flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

    Plasmid

    #133334

    Purpose

    for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

    Depositor

    Michael Laub

    Article

    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

    Insert

    sgRNA_ctrA
    

    Use

    CRISPR

    Promoter

    constitutive

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL4.23-IL2RA CaRE5 (plus strand)

    Plasmid

    #91841

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0618)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE5 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. 1201 pAdTrack FKHR H215R AAA

    Plasmid

    #9039

    Depositor

    William Sellers

    Article

    Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

    Insert

    FKHR H215R AAA (FOXO1 Human)
    

    Use

    Adenoviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H215R T24A S256A S319A

    Available Since

    Sept. 28, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pGL4.23-IL2RA CaRE3 (minus strand)

    Plasmid

    #91835

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0612)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE3 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    rs7893324 A>G, rs7090530 G>T, rs7090512 A&g…

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNAS1B split mCherry N-MESVD/TRAP(RK)-C

    Plasmid

    #61967

    Purpose

    Duet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVD

    Depositor

    Lynne Regan

    Article

    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

    Inserts

    N-mCherry-MESVD
    TRAP(RK)-C-mCherry
    

    Expression

    Bacterial

    Promoter

    PBAD and PLtetO

    Available Since

    Feb. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGal-Trans-Myc-HA-GFP11-KDEL

    Plasmid

    #177724

    Purpose

    ER protein collagen beta (1-O) galactosyltransferase with a C-terminal Myc tag, an HA tag and the eleventh β-strand of GFP

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
    

    Tags

    GFP11, HA, KDEL, and Myc

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4.23-IL2RA CaRE5 (minus strand)

    Plasmid

    #91836

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0613)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE5 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL4.23-IL2RA CaRE2 (plus strand)

    Plasmid

    #91846

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0623)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE2 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    rs6602425 T>G, T>C at chr10:6117603

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. (P79) OCN/Wnt 10b transgene

    Plasmid

    #25878

    Depositor

    Ormond MacDougald

    Insert

    Wnt10b (Wnt10b Mouse)
    

    Use

    Mouse Targeting

    Tags

    B-globin intron/poly A coding sequence and OCN pr…

    Expression

    Mammalian

    Available Since

    Oct. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pIN3 (gpdA-cTerm-Citrine2-ptrA)

    Plasmid

    #188737

    Purpose

    Expresses Citrine2 for C-terminal tagging

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    gdpA-Citrine2 PtrA
    

    Use

    Filamentous fungal expression

    Tags

    Citrine2

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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