We narrowed to 20,115 results for: IRE;
-
Plasmid#86062PurposeA mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus Myc-tag.DepositorInsertFibrocystin (Pkhd1 Mouse)
TagsMycExpressionMammalianMutationcontains luminal and transmembrane domains of CD8…PromoterCMVAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY036_ATP1A1_G3
Plasmid#86617PurposeExpresses the ATP1A1 G3 crRNA in combination with AsCpf1-3xHA to target ATP1A1 exon 4. U6-crRNA(ATP1A1 G3)-CBh-AsCpf1DepositorInsertATP1A1 G3 crRNA + AsCpf1-3xHA
UseCRISPR; Co-selection via nhej using ouabainTags3xHAExpressionMammalianPromoterCBhAvailable SinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry MALAT1_Enhancer.4
Plasmid#78539PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralExpressionMammalianPromoterU6 and U6 (for expressing sgRNA)Available SinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry MALAT1_Enhancer.3
Plasmid#78538PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralExpressionMammalianPromoterU6 and U6 (for expressing sgRNA)Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry MALAT1_Exon.3
Plasmid#78542PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralExpressionMammalianPromoterU6 and U6 (for expressing sgRNA)Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDECKO-mCherry MALAT1_Enhancer.2
Plasmid#78537PurposeExperimental plasmidDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralExpressionMammalianPromoterU6 and U6 (for expressing sgRNA)Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNC2
Plasmid#72438PurposeProduces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1); NCAIR synthetase (EcPurK)DepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
ExpressionBacterialMutationPro64 to Arg; Asp65 to His; Gln206 to ArgPromoterT7 and n/aAvailable SinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160E)
Plasmid#66846PurposeExpresses soybean lipoxygenase-1 S160E mutant with N-term HistagDepositorTagsHis-tagExpressionBacterialMutationS160EPromoterT7lacAvailable SinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30Xa-SBL1(160S,NoCys)
Plasmid#66877PurposeExpresses a cysteine-free version of WTsoybean lipoxygenase-1 160S (4Cys to Ser) with N-term HistagDepositorInsertsoybean lipoxygenase-1
TagsHistagExpressionBacterialMutationfour (all) cysteines mutated to serinePromoterT7lacAvailable SinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB682
Plasmid#60143PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationA4VPromoterMET17prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-hRIP1-FL I541P
Plasmid#61381PurposeExpresses Human full length RIPK1 with I541P mutation in the mammalian cellsDepositorInsertFull length human RIP1with I541P mutation (RIPK1 Human)
TagsEGFP and HA tagExpressionMammalianMutationchanged I541 to PAvailable SinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Flag-Myc-Qin-Tudor-attB
Plasmid#44210DepositorInsertQin-Tudor-domain (qin Fly)
UseFly transformationTagsFlag and MycMutationlacks E3 domain on N-term (del aa1-274)Available SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
DIRAS2
Plasmid#55670Purposeexpression of mouse DIRAS2DepositorAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 6xUTR
Plasmid#40761PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert6 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 2xUTR
Plasmid#40757PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert2 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 4xUTR
Plasmid#40759PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert4 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 5xUTR
Plasmid#40760PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert5 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 1xUTR
Plasmid#40756PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsertCXCR4 small RNA target site
UseLuciferaseTagsluciferaseMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
TRS6E1b-luc(-732/-721) mutant1
Plasmid#45384DepositorInsert6 copies of hPAI1 promoter -732/-721 mutated at -732/-731
UseLuciferaseExpressionMammalianMutationmutated -732/-731 from ag to tcAvailable SinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
Abr-pLEF
Plasmid#38157DepositorAvailable SinceAug. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP-GW1D1aMut(W>A)
Plasmid#21522DepositorInsertTNRC6A (TNRC6A Human)
TagsGFPExpressionMammalianMutationcontain aa254-503, mutate every tryptophan (total…Available SinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-rB90
Plasmid#18959DepositorInsertBrevican C-terminus (Bcan Rat)
ExpressionMammalianMutationRat brevican C-terminus: Signal peptide aa 1-22 …Available SinceDec. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-EF1+EF2
Plasmid#18080DepositorAvailable SinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cerulean-Epac2(R53A)-Venus
Plasmid#138197PurposeFRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(R53A)-Venus (Rapgef4 Mouse, Synthetic)
TagsCerulean and VenusExpressionMammalianMutationContains arginine-to-alanine substitution at resi…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cerulean-Epac2(R466A)-Venus
Plasmid#138199PurposeFRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(R466A)-Venus (Rapgef4 Mouse, Synthetic)
TagsCerulean and VenusExpressionMammalianMutationContains arginine-to-alanine substitution at resi…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cerulean-Epac2(L426W)-Venus
Plasmid#138200PurposeFRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(L426W)-Venus (Rapgef4 Mouse, Synthetic)
TagsCerulean and VenusExpressionMammalianMutationContains leucine-to-tryptophan substitution at re…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pYL192 (TRV1)
Plasmid#148968PurposeTobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)DepositorInsertTRV RNA1
UsePlant vector with trv rna1; this vector used with…MutationP697S mutation in putative replicase. S239G and S…Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
LAMP1-mRFP-FLAG
Plasmid#34611Purpose3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tagDepositorAvailable SinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
CAHS2parrc-mCh-2xFKBP (pBS1109)
Plasmid#185304PurposeFor the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertCAHS2parrc
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP‐RNASEH1
Plasmid#108699PurposeFor expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cellsDepositorAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only