We narrowed to 28,934 results for: Tat

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plentiCRISPRv2-sgEGFP

Plasmid

#86153

Purpose

Lentivirus carrying Cas9/CRISPR for cut in GFP (used as control)

Depositor

Roland Friedel

Article

Huang et al Commun Biol. 2021 Jan 29;4(1):145. doi:

Insert

EGFP

Use

CRISPR and Lentiviral

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA1L

Plasmid

#19484

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA1L (HSPA1L Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pRha-ABE7.10-SpCas9-NG

Plasmid

#170663

Purpose

Expresses the base editor ABE7.10-SpCas9-NG in bacterial cells

Depositor

David Liu

Article

Krishnamurthy et al Nucleic Acids Res. 2021 Oct 11;49(18):10

Insert

ABE7.10-SpCas9-NG

Tags

8xHIS and BP-NLS

Expression

Bacterial

Promoter

pRhaBAD

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-p53-shRNA-427

Plasmid

#25636

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Insert

p53-shRNA-427 (TP53 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pEF6-myc-TBC1D15

Plasmid

#79148

Purpose

mammalian expression of TBC1D15

Depositor

Aimee Edinger

Article

Peralta et al J Biol Chem. 2010 May 28. 285(22):16814-

Insert

TBC1D15 (Tbc1d15 Mouse)

Tags

His, Myc, and V5

Expression

Mammalian

Mutation

S616N (please see depositor comment below)

Promoter

EF-1alpha

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 IntS12

Plasmid

#210521

Purpose

Expresses GST-tagged Drosophila IntS12

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

IntS12 (IntS12 Fly)

Tags

GST

Expression

Bacterial

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pc DNA FRT TO- APPSwed/Ind

Plasmid

#114194

Purpose

Creation of a stable cell line expressing APP mutation (AD in vitro study)

Depositor

Aleksandra Radenovic

Insert

Amyloid-Precursor-Protein (APP Human)

Expression

Mammalian

Mutation

Popular mouse model, overexpress 2 mutations: Swe…

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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Green Pegassos

Plasmid

#163114

Purpose

Expresses biosensors for pyruvate in mammalian cells

Depositor

Takashi Tsuboi

Article

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Pegassos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDuet TDP43 WT

Plasmid

#27462

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Tags

GST

Expression

Bacterial

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LacR-TopBP1

Plasmid

#31317

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

Insert

TopBP1 (TOPBP1 Human)

Tags

FLAG and LacR

Expression

Mammalian

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pCLASP_mScarlet_CLASP_RGS2membrane

Plasmid

#133086

Purpose

Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl

Depositor

Hana El-Samad

Article

Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

Insert

mScarlet-CLASP

Expression

Bacterial and Yeast

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pINDUCER13 miR-200c-141

Plasmid

#81020

Purpose

Lentiviral vector containing doxycycline inducible human miR-200c. Puromycin selectable.

Depositor

Jeffrey Rosen, Thomas Westbrook

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

miR-200c (MIR200C Human)

Use

Lentiviral

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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Pb-TRE3G-SpCas9-IRES-blast

Plasmid

#195506

Purpose

Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4687

Plasmid

#200783

Purpose

Pnpr-4 ins-22::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 pHluorin

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ins-22 (ins-22 Nematode)

Tags

pHiuorin

Expression

Worm

Promoter

Pnpr-4

Available Since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-ALK7 K222R

Plasmid

#80887

Purpose

mammalian expression of ALK7 K222R

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

ALK7 (Acvr1c Rat)

Tags

HA

Expression

Mammalian

Mutation

K222R

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pRRG227-Wnt1

Plasmid

#99073

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Wnt1 (also known as WntP-1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Wnt1 (also known as WntP-1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pJCC_075 AsCas12a D908A

Plasmid

#171668

Purpose

For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a D908A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 908 to alanine

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHelper-CRISPRi-sgRNA

Plasmid

#221135

Purpose

Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

Depositor

Craig Roy

Article

Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

Insert

sgRNA-scaffold with dCas9 handle

Use

CRISPR

Expression

Bacterial

Promoter

J23119(SpeI)

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRRG217-soxB

Plasmid

#99086

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea soxB (also known as soxB1-1)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

soxB (also known as soxB1-1) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMD19-PBBa_J23117-sgRNA-Spr

Plasmid

#190793

Purpose

Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Insert

sgRNA (dummy)

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23117

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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