We narrowed to 18,729 results for: MUT
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Plasmid#50798PurposeExpression of tdTomato under the control of the TRE-promoterDepositorInsertTREtight driven tdTomato fluorescent protein
UseMutli-speciesPromoterTREtightAvailable SinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCas9/VRQR-sgRNA (BbsI)
Plasmid#129725PurposeExpressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAMDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
p70a-T7RNAP
Plasmid#105683PurposeExpresses T7 polymerase in cell-free TXTL.DepositorInsertT7 polymerase
ExpressionBacterialPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p5'Entry-lck-5.5kb
Plasmid#58890Purposeprovides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenesDepositorInsertzebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
UseGateway 5' entry cloneMutationmutated single nucleotide (T-->C, 13th nucleot…Promoterlymphocyte-specific protein tyrosine kinase (lck)…Available SinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Streptavidin-Dead
Plasmid#20859PurposeDead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).DepositorInsertStreptavidin-Dead
ExpressionBacterialMutationTriple mutant of wt Streptavidin N23A,S27D,S45AAvailable SinceApril 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-hM3D(Gq)-mCherry
Plasmid#50460PurposeDouble floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoterDepositorInserthM3D(Gq)-mCherry (CHRM3 Human)
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterEF1aAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
Tags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT04
Plasmid#223376PurposeT-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.DepositorInsertZmUbi-SpCas9-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-Rncp-iGluSnFR1
Plasmid#107336PurposeRed fluorescent glutamate sensor with ncp topology. Membrane-displayedDepositorInsertRncp-iGluSnFR1
ExpressionMammalianPromoterCMVAvailable SinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-slr0230-PL22-sgRNANT1-KmR
Plasmid#73224PurposeContains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coliDepositorInsertPL22-sgRNA NT1
ExpressionBacterialPromoterPL22Available SinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT03
Plasmid#223375PurposeT-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-SpCas9-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
Integrin-β6 Headpiece I270C
Plasmid#159638PurposeContains integrin beta 6 residues 1–474 with I270C mutationDepositorInsertIntegrin beta 6 headpiece I270C
Tags6x HisExpressionMammalianMutationI270CAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET16B.Pfu
Plasmid#12509PurposeBacterial expression of Pfu DNA polymeraseDepositorInsertPfu DNA polymerase
ExpressionBacterialMutationY218H, Q462R, F533L, K559I and V683A (mutations w…Available SinceSept. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Core Traptavidin
Plasmid#26054DepositorInsertCore Traptavidin
ExpressionBacterialMutationStreptavidin with 2 amino acid mutations: S52G R5…Available SinceAug. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Casp3 C163A-myc
Plasmid#11814DepositorAvailable SinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEMS-II
Plasmid#204765PurposeGEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)DepositorInsertEGFP-m6Alinker-DHFR
Mutationmutated all RACs in EGFP to RGC/RCCPromoterCMVAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAM1
Plasmid#60487PurposeThis plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genomeDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMD2.G-K47Q/R354Q
Plasmid#207323PurposeVSV-G mutant with abolished receptor binding, but maintained fusogenic capacityDepositorInsertVSV-G (VSIVgp4 Vesicular Stomatitis Virus)
ExpressionMammalianMutationK47Q/R354QPromoterCMV promoterAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
p426MET25_sfpHluorin (MRV55)
Plasmid#115697Purposesuperfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)DepositorInsertsfpHluorin
ExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOlyA(WT)
Plasmid#132391PurposeOstreolysin A for sensing sphingomyelin/cholesterol complexesDepositorInsertOstreolysin A wild-type
TagsHis6 tagExpressionBacterialMutationMutations: C62S, C94S, S151CAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMUH_unc84_tdTFlag
Plasmid#46024DepositorInsertUNC-84 (unc-84 )
Use10xuas-phi31-expression vectorTagstdTomatoMutationTwo amino acid mutations (F279L and L500P) compar…Promoter10XUASAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM3D(Gq)-mCherry
Plasmid#50476PurposeGq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorHas ServiceAAV1, AAV2, AAV5, AAV8, and AAV9InserthM3D(Gq)-mCherry (CHRM3 Human)
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT07
Plasmid#223379PurposeT-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.DepositorInsertAtUBQ10-SpRY-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX458-Dual-Guide-Donor-Cas9-H2B-mCherry
Plasmid#175570PurposeDonor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.DepositorTypeEmpty backboneUseCRISPRMutationXbaI site added upstream of U6 promotor. G to C …Available SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_SOX15
Plasmid#167792PurposeExpresses SOX15 in mammalian cellsDepositorAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-ND1-1397C-AD
Plasmid#183892PurposeExpresses TALED_Left-ND1-1397C-AD in mammalian cellsDepositorInsertTALED for ND1 mutation
ExpressionMammalianPromoterpCMVAvailable SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
TALED_Right-ND1-1397N
Plasmid#183898PurposeExpresses TALED_Right-ND1-1397N in mammalian cellsDepositorInsertTALED for ND1 mutation
ExpressionMammalianPromoterpCMVAvailable SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-ND1-AD-E1347A
Plasmid#183894PurposeExpresses TALED_Left-ND1-AD-E1347A in mammalian cellsDepositorInsertTALED for ND1 mutation
ExpressionMammalianPromoterpCMVAvailable SinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-MacroGreen
Plasmid#160665PurposeExpresses GFP fusion of a multisite mutant of the Af1521 proteinDepositorInsertAf1521 modified synthetic construct eGFP fusion
TagseGFP and hexahistidine_TEV recognitionExpressionBacterialPromoterLacIAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM4D(Gi)-mCherry
Plasmid#50477PurposeGi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorHas ServiceAAV1, AAV2, AAV5, AAV8, and AAV9InserthM4D(Gi)-mCherry (CHRM4 Human)
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable SinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only