Addgene

We narrowed to 21,474 results for: ESC;

Showing: 11041 - 11070 of 21474 results

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LentiCRISPRv2-sgSORCS2-G2

Plasmid

#118416

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting Human SORCS2

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA SORCS2 Human (SORCS2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pPK1E-1+t-gfp

Plasmid

#48131

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Actin-C-18

Plasmid

#55400

Purpose

Localization: Actin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Actin (ACTB Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pCEP-WAF1-ES

Plasmid

#16449

Depositor

Bert Vogelstein

Article

el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

Insert

WAF1 stop codon mut (CDKN1A Human)

Use

Tags

Expression

Mammalian

Mutation

stop codon at amino acid 49 instead of tryptophan…

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pLenCMV-PMMars1CyAVPR2

Plasmid

#75226

Purpose

Lentiviral transfer vector encoding Mars1Cy-tagged AVPR2

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

Mars1Cy-AVPR2 (AVPR2 Synthetic)

Use

Lentiviral

Tags

HA epitope

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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AI-CN590

Plasmid

#62596

Purpose

KCTD13 N-terminus disruption by homolgy-directed repair

Depositor

Joshua Grimley

Article

Martinez et al Nucleic Acids Res. 2015 Mar 11. pii: gkv

Insert

Cerulean-P2A-PuroR (KCTD13 Human)

Use

TALEN; Homology-directed repair donor

Tags

Expression

Mammalian

Mutation

Promoter

hEF1alpha

Available since

March 24, 2015

Availability

Academic Institutions and Nonprofits only

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mCerulean3-ER-5

Plasmid

#55418

Purpose

Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

ER (CALR Human)

Use

Tags

mCerulean3

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
tdEos-VASP-5

Plasmid

#57685

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

VASP

Use

Tags

tdEos

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pJK597

Plasmid

#71121

Purpose

Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coli

Depositor

T. J. Kappock

Article

Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

Insert

formyl-CoA:oxalate CoA-transferase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-PM BRD7 sh3

Plasmid

#41930

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
MSCV-PM BRD7 sh2

Plasmid

#41928

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
mCardinal-Cx43-7

Plasmid

#56157

Purpose

Localization: Gap Junctions, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Cx43

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pPK1E-2+t-gfp

Plasmid

#48132

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pAK208_2xBRD9BD-IFP2.0C

Plasmid

#233734

Purpose

Expresses BRD9 Bromodomain as tandem double domain fused to C-terminal part of IFP2.0 for histone acetylation detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Köhler et al bioRxiv 2025.02.10.637448

Insert

BRD9 (BRD9 Human)

Use

Tags

IFP2.0 C-terminal part

Expression

Mammalian

Mutation

Bromodomain cloned twice in tandem

Promoter

CMV

Available since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAK208_2xBRD9BD_Y57A-IFP2.0C

Plasmid

#233733

Purpose

Expresses BRD9 Bromodomain as tandem double domain Y57A mutant fused to C-terminal part of IFP2.0 as negative control for plasmid 233734

Depositor

Albert Jeltsch

Article

Köhler et al bioRxiv 2025.02.10.637448

Insert

BRD9 (BRD9 Human)

Use

Tags

IFP2.0 C-terminal part

Expression

Mammalian

Mutation

Bromodomain cloned twice in tandem with Y57A muta…

Promoter

CMV

Available since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-miR30-shJAG1 #1

Plasmid

#171196

Purpose

Retroviral vector for U6 promoter driven expression empty miR30 based shJAG1 #1 (to be used in conjunction with Phoenix packaging cells).

Depositor

Masashi Narita

Article

Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

Insert

shJAG1 #1

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-miR30-shJAG1 #4

Plasmid

#171197

Purpose

Retroviral vector for U6 promoter driven expression empty miR30 based shJAG1 #4 (to be used in conjunction with Phoenix packaging cells).

Depositor

Masashi Narita

Article

Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

Insert

shJAG1 #4

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP(ABC1D)-ECS-SpyCatcher003

Plasmid

#234545

Purpose

to express SpyCatcher on the extracellular side of astrocytic plasmamembrane

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-SC

Use

AAV

Tags

Expression

Mutation

None

Promoter

mGFAP(ABC1D)

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-SpyCatcher003-GINKO2

Plasmid

#234544

Purpose

to express a potassium sensor tagged with SpyCatcher in the liver

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

IgKL-SpyCatcher003-GINKO2

Use

AAV

Tags

Expression

Mutation

None

Promoter

P3

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP(ABC1D)-ECS-dSpyCatcher003

Plasmid

#234546

Purpose

to express dSpyCatcher on the extracellular side of astrocytic plasmamembrane

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-dSC

Use

AAV

Tags

Expression

Mutation

None

Promoter

mGFAP(ABC1D)

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-SpyCatcher003-SEP

Plasmid

#234541

Purpose

to express a pH sensor tagged with SpyCatcher in the liver

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

IgKL-SpyCatcher003-SEP

Use

AAV

Tags

Expression

Mutation

None

Promoter

P3

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SnoopTag

Plasmid

#225195

Purpose

Used for adding C-terminal SnoopTag to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SnoopTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS∆tum

Plasmid

#225198

Purpose

Used to create a markerless deletion of the E. coli HS tum gene

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

tum homology arms

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_lexA(Ind-)

Plasmid

#225199

Purpose

Used to create a non-inducible lexA by introducing a G85D mutation in E. coli HS

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

lexA homology arms

Use

Tags

Expression

Bacterial

Mutation

lexA G85D

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SpyTag

Plasmid

#225193

Purpose

Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAX1_HS_gpN:SpyTag003

Plasmid

#225194

Purpose

Used for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag003

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP

Plasmid

#232754

Purpose

Expresses EGFP

Depositor

Isaac Hilton

Article

Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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dCas9-EGFP

Plasmid

#232761

Purpose

Expresses dCas9-EGFP

Depositor

Isaac Hilton

Article

Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

Insert

dCas9-EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-UbC-VP64-dCas9-VP64

Plasmid

#232179

Purpose

Lentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cells

Depositor

Charles Gersbach

Article

Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

Insert

VP64-dCas9-VP64

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

hUbC

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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GB4635

Plasmid

#215263

Purpose

Module for the expression of HISPS under the copper inducible CBS:mDFR promoter and H3H under the red light regulated Etr8:CMVmin promoter and the constitutive expression of LUZ, CPH,EGFP and p19

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS4x:mDFR:HISPS-Etr4:mDFR:H3HSF-LUZ-CPH-EGFP-p19

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge