We narrowed to 30,127 results for: LIS;

1. pSWEET-bgaB

   Plasmid

   #210272

   Purpose

   Xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.

   Depositor

   Eric Brown

   Article

   Bhavsar et al Appl Environ Microbiol. 2001 Jan;67(1):4

   Insert

   bgaB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PxylA

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-3xFLAG-NLS-SpCas9-NLS

   Plasmid

   #51307

   Purpose

   Cas9 expression plasmid

   Depositor

   Yonglong Chen

   Article

   Guo et al Development. 2014 Jan 8.

   Insert

   3xFLAG-NLS-SpCas9-NLS
   

   Use

   CRISPR; Xenopus tropicalis expression

   Tags

   3x Flag and NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHelper_TetR/pTet-Tnpase (camR)

   Plasmid

   #213910

   Purpose

   ATc-inducible himar1C9 transposase expression - cam resistance

   Depositor

   James Collins

   Article

   English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

   Insert

   TetR/ptet ATc-inducible himar1C9 transposase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pERB215

   Plasmid

   #61499

   Purpose

   Const. Halotag-GFP-Nuf2 + mCherry-DHFR

   Depositor

   Michael Lampson, David Chenoweth

   Article

   Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

   Inserts

   HaloTag-GFP-Nuf2
   mCherry-DHFR
   

   Use

   Tags

   HaloTag and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCGN R-Ras 43N

   Plasmid

   #14731

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

   Insert

   R-ras (RRAS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   43N

   Promoter

   Available since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pCGN R-Ras 38V

   Plasmid

   #14728

   Depositor

   Adrienne Cox

   Article

   Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

   Insert

   R-ras (RRAS Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   38V

   Promoter

   Available since

   Aug. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pMXs-mRFP1

   Plasmid

   #21315

   Purpose

   MoMLV-based Retroviral vector, expresses mRFP1

   Depositor

   James Ellis

   Article

   Hotta et al Nat Methods. 2009 May . 6(5):370-6.

   Insert

   monomeric Red Fluorescence Protein 1
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6xO/S luc

   Plasmid

   #69445

   Purpose

   detection/assessment of OCT4 and SOX2 transcription factor activity

   Depositor

   Lisa Dailey

   Insert

   minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pB8_4-3i-ATP8_v8_AGKS

   Plasmid

   #191513

   Purpose

   Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right)

   Depositor

   David Liu

   Article

   Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

   Insert

   v8 ZF-DdCBE 4-3i-ATP8 AGKS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pB5_R8-3i-ATP8_v8_AGKS

    Plasmid

    #191510

    Purpose

    Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    v8 ZF-DdCBE R8-3i-ATP8 AGKS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MK1283

    Plasmid

    #71428

    Purpose

    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709

    Depositor

    Howard Salis

    Article

    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

    Insert

    Mixed Feedback Loop version of the UBER system
    

    Use

    Tags

    Expression

    Mutation

    T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …

    Promoter

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3 Rod Opsin

    Plasmid

    #109361

    Purpose

    Human rod opsin

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Rod opsin (RHO Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. His6-SUMO-pET28a-EfaCas1

    Plasmid

    #112793

    Purpose

    To express enterococcus faecalis Cas1 protein in E. coli

    Depositor

    Ailong Ke

    Article

    Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

    Insert

    EfaCas1
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBS-67Sma

    Plasmid

    #49342

    Purpose

    Chlamy Cp transformation for transgene integration via double-homologous recombination into the rbcL-psaB intergenic region

    Depositor

    Anastasios Melis

    Article

    Chen et al Plant Biotechnol J. 2013 Sep;11(7):818-2

    Insert

    EcoRI 5.8 kB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHelper_TetR/pTet-Tnpase (carbR)

    Plasmid

    #213911

    Purpose

    ATc-inducible himar1C9 transposase expression - carb/amp resistance

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    TetR/ptet ATc-inducible himar1C9 transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLys-M1

    Plasmid

    #109382

    Purpose

    Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pARABAD-VioB-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-UbC-eGFP-F

    Plasmid

    #71545

    Purpose

    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.

    Depositor

    Pantelis Tsoulfas

    Insert

    eGFP-F
    

    Use

    AAV

    Tags

    contains 20- amino-acid farnesylation signal from…

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MK1274

    Plasmid

    #71431

    Purpose

    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.

    Depositor

    Howard Salis

    Article

    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

    Insert

    Mixed Feedback Loop version of the UBER system
    

    Use

    Tags

    Expression

    Mutation

    T7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…

    Promoter

    Available since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEM705 Gso (Cys)

    Plasmid

    #109375

    Purpose

    GsX chimera with Gαo C-terminus (Pertussis toxin sensitive)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gso chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Cys at position 391 (Pertussis Toxin Sensitive)

    Promoter

    CAG

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEM705 Gsi (Cys)

    Plasmid

    #109373

    Purpose

    GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gsi chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Cys at position 391 (Pertussis Toxin Sensitive)

    Promoter

    CAG

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCGN R-Ras wt

    Plasmid

    #14727

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

    Insert

    R-ras (RRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

22. pSB1C3-H3

    Plasmid

    #109381

    Purpose

    Arabinose inducible VioB-mCherry expression (weaker RBS). Used to test burden response in E. coli

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pARABAD-VioB-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pERB221

    Plasmid

    #61502

    Purpose

    const. CenpB-GFP-Halo + mCherry-DHFR

    Depositor

    Michael Lampson, David Chenoweth

    Article

    Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

    Inserts

    CenpB-GFP-Halo
    mCherry-DHFR
    

    Use

    Tags

    HaloTag and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pERB217

    Plasmid

    #61500

    Purpose

    Const. Halotag-GFP-Mito + mCherry-DHFR

    Depositor

    Michael Lampson, David Chenoweth

    Article

    Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

    Inserts

    HaloTag-GFP-Mito
    mCherry-DHFR
    

    Use

    Tags

    HaloTag and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pB6N_RB38-Mt-tk_v8_AGKS

    Plasmid

    #191514

    Purpose

    Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (N-terminal DddAN, Right)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    v8 ZF-DdCBE RB38-Mt-tk AGKS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pB7N_LT51-Mt-tk_v8_AGKS

    Plasmid

    #191515

    Purpose

    Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (N-terminal DddAC, Left)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    v8 ZF-DdCBE LT51-Mt-tk AGKS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Sc eRF1 (WT)

    Plasmid

    #175413

    Purpose

    Encodes His-MBP-TEV-WT eRF1

    Depositor

    Joseph Puglisi

    Article

    Lawson et al Science. 2021 Aug 20;373(6557):876-882.

    Insert

    eRF1 (SUP45 Budding Yeast)
    

    Use

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a-R4-uAb

    Plasmid

    #101801

    Purpose

    Bacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb

    Depositor

    Matthew DeLisa

    Article

    Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55

    Insert

    R4-uAb
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEM705 Gst (Cys)

    Plasmid

    #109376

    Purpose

    GsX chimera with Gαt C-terminus (Pertussis toxin sensitive)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gst chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Cys at position 391 (Pertussis Toxin Sensitive)

    Promoter

    CAG

    Available since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pERB219

    Plasmid

    #61501

    Purpose

    Const. Halotag-GFP-PACT + mCherry-DHFR

    Depositor

    Michael Lampson, David Chenoweth

    Article

    Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

    Inserts

    HaloTag-GFP-PACT
    mCherry-DHFR
    

    Use

    Tags

    HaloTag and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge