We narrowed to 30,802 results for: REP

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Lentiviral-FOP-dGFP-reporter

Plasmid

#14885

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

mutant LEF-1/TCF-responsive promoter (Ctnnb1 )

Use

Lentiviral

Tags

d2 eGFP

Expression

Mammalian

Mutation

mutant TCF/LEF binding sites

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CDK6

Plasmid

#172614

Purpose

Expresses 2xFLAG-2xSTREP-tagged CDK6 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK6 (CDK6 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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7b sgRNA for EJ7-GFP reporter

Plasmid

#113624

Purpose

sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7b sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pLenti-SF3B1-K700E-reporter

Plasmid

#200318

Purpose

A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

Depositor

Lili Wang

Article

Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

Insert

BSD-2A-EGFPi

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV_3C-Twin-Strep

Plasmid

#113900

Purpose

Mammalian protein expression

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

SFFV

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

Plasmid

#198750

Purpose

Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Article

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - SmallBiT

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pUPD2:35S-TMV-Rep-MP

Plasmid

#106217

Purpose

Provides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp polymerase with intron, movement protein and sgCP promoter) as level 0 GoldenBraid part (GGAG-AATG)

Depositor

Tomáš Moravec

Insert

Infectious clone of Tobacco mosaic virus, 5' part

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CDK2

Plasmid

#172616

Purpose

Expresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK2 (CDK2 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196)-Strep

Plasmid

#196208

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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T7 promoter + terminators reporter plasmid

Plasmid

#156456

Purpose

A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

NeoR/KanR
TcR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7 and tet

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pPICZ A-Strep-Opa1 (SB251)

Plasmid

#227600

Purpose

Inducible expression of Strep-tagged Opa1 in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Tags

Twin-StrepTag

Expression

Yeast

Mutation

contains aa 253-960 only

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(EF1a)-TTN

Plasmid

#172327

Purpose

LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

EF1-alpha

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCE046-SiT-Cas12a-[Repr]

Plasmid

#128132

Purpose

Express both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcript

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-[Repr]-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-C-TEV-Twin-Strep

Plasmid

#202527

Purpose

Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-S in pAR9 (rep/cap)

Plasmid

#174539

Purpose

encodes AAV-S capsid for efficient gene delivery to the mammalian cochlea

Depositor

Casey Maguire

Article

Ivanchenko et al Mol Ther Methods Clin Dev. 2021 Mar 29;2

Insert

VP1 of AAV9 containing the AAV-S 7-mer peptide insert

Use

AAV

Expression

Mammalian

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-TS2126 RnlA-Strep

Plasmid

#159350

Purpose

Express TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectively

Depositor

Takashi Ito

Article

Hisano et al BMC Biol. 2021 Oct 14;19(1):225. doi: 10

Insert

TS2126 RNA ligase

Tags

His6 and Strep

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-2xSTREP-CCND2

Plasmid

#172629

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND2 (CCND2 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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7TFP CDH1 mutant reporter

Plasmid

#91703

Purpose

lentiviral luciferase reporter containing CDH1 promoter with mutant E-boxes

Depositor

Bob Weinberg

Article

Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

Insert

CDH1 mutant promoter (CDH1 Human)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutant E-boxes

Promoter

CDH1 mutant

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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betaTrCP mVenus mutant reporter

Plasmid

#192459

Purpose

Fluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.

Depositor

Steven Cappell

Article

Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

Insert

CdC25B

Use

Lentiviral

Tags

SV40 NLS and mVenus

Expression

Mammalian

Mutation

aa198-338 of CDC25B. betaTrCP degron sequence (aa…

Promoter

EF1alpha

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Lepr peak reporter-eGFP

Plasmid

#196584

Purpose

Cis-regulatory region of mouse Lepr gene driven a eGFP reporter with hGFP driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Insert

eGFP

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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