We narrowed to 29,515 results for: REP

1. psubCAG-WPRE-hVEGF-D-Strept

   Plasmid

   #119227

   Purpose

   Plasmid encodes Strep-tagged full-length (unprocessed) VEGF-D of human origin to be used for stimulation of lymphangiogenesis in mice in vivo.

   Depositor

   Kari Alitalo

   Insert

   VEGFD (VEGFD Human)
   

   Use

   AAV

   Tags

   Strept

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. eGFP Y93H AC editing reporter

   Plasmid

   #198883

   Purpose

   Express eGFP fluorescence after Y93H correction and mCherry as transfection control

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Y93H, Gly92(GGT-to-GGA)

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2

   Plasmid

   #183741

   Purpose

   Expresses Ki67 (8 repeats+LR)-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   MKI67 LR domain + 8 Ki-67 repeats

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETNKI-strepII-3C-LIC-Amp

   Plasmid

   #108707

   Purpose

   Expression of a StrepII-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Use

   Tags

   StrepII-3C protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRE3G-c-fos-iRFP-B3-repRNA-A

   Plasmid

   #159926

   Purpose

   Timestamp driven by a c-Fos promoter, with RNA barcode CTG, AKA 187.

   Depositor

   Fei Chen

   Article

   Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

   Insert

   iRFP-Timestamp A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-hSyn-DIO-mCReP

   Plasmid

   #167503

   Purpose

   Conditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)

   Depositor

   Nicole Calakos

   Article

   Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

   Insert

   Ppp1r15b (Ppp1r15b Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-CAG_3C-Twin-Strep

   Plasmid

   #113901

   Purpose

   Mammalian protein expression

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bxbi GFP BAC Reporter

   Plasmid

   #78212

   Purpose

   Synthetic gene circuit

   Depositor

   Timothy Lu

   Article

   Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

   Insert

   synthetic circuit
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6xHis(TEV)-Drp1-StrepII

   Plasmid

   #174422

   Purpose

   Expresses human Drp1 Isoform 3 in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Use

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACYC-T7-Nla-repeat-BbsI

    Plasmid

    #180215

    Purpose

    For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-K9 histone methylation reporter

    Plasmid

    #22866

    Depositor

    Alice Ting

    Article

    Lin et al J Am Chem Soc. 2004 May 19. 126(19):5982

    Insert

    K9 HMR
    

    Use

    Tags

    CFP and YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pPuro3.1(+)_Strep-Lrrk2-Myc

    Plasmid

    #161583

    Purpose

    expression of full-length mouse Lrrk2 with C-terminal Myc tag

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX380 Treponema denticola Cas9

    Plasmid

    #68317

    Purpose

    Treponema denticola Cas9

    Depositor

    Feng Zhang

    Insert

    TdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. eGFP Y93H TC editing reporter

    Plasmid

    #198882

    Purpose

    Express eGFP fluorescence after Y93H correction and mCherry as transfection control

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Y93H

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastbac-Dual-StrepII-BARD1

    Plasmid

    #137166

    Purpose

    Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system

    Depositor

    Andrew Deans

    Article

    Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

    Insert

    BARD1 (BARD1 Human)
    

    Use

    Tags

    StrepII

    Expression

    Insect

    Mutation

    fully synthetic, codon optimized for insect expre…

    Promoter

    p10

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-StrepHA-SUMO2(Q87R)

    Plasmid

    #66868

    Purpose

    Mammalian expression of SUMO2(Q87R) with a strep and an HA tag

    Depositor

    Ying Liu

    Article

    Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

    Insert

    SUMO2 (SUMO2 Human)
    

    Use

    Tags

    HA and Strep

    Expression

    Mammalian

    Mutation

    Q87R

    Promoter

    CMV

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. miRFP718nano-based inflammation reporter

    Plasmid

    #195753

    Purpose

    Reporter of inflamation

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    NF-kB-RE-miRFP718nano
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

    Plasmid

    #198750

    Purpose

    Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - SmallBiT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

    Plasmid

    #198751

    Purpose

    Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

    Depositor

    Maarten Merkx

    Article

    van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

    Insert

    SpdCas9 - LargeBiT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    D10A, H840A (nuclease inactivating mutations in &…

    Promoter

    T7

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCVL.Active/Repressed TLR (Sce)

    Plasmid

    #45575

    Purpose

    Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Inserts

    eGFP with I-Sce I TS
    +3 mCherry
    

    Use

    Lentiviral

    Tags

    T2A dislinker and iRFP

    Expression

    Bacterial and Mammalian

    Mutation

    embedded I-Sce I TS from 163-185

    Promoter

    SFFV

    Available since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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