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Showing: 721 - 740 of 52635 results

  1. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus replaced NBCe C-terminus (NT94)

    Plasmid
    #50912
    Purpose
    Expresses human NKCC1 with truncation of N-terminus, mutations at P676C I730C and C-terminus replaced with that of NBCe. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    P676C and I730C; N-term deletion of aa13-222; C-t…
    Promoter
    CMV
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    50912-plasmid-map-sequence-id-76385

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  2. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations P676C I730C (NT95)

    Plasmid
    #50913
    Purpose
    Expresses human NKCC1 with mutated Ser and Thr residues in the N-terminus and mutations at P676C I730C. Contains N-terminal 3xFLAG-YFP tag for expression in mammalian cells
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    S150N S155Q S170Q T177A S183R S193E T203A T205A T…
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50913-plasmid-map-sequence-id-76386

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  7. pSB142 - pL0_ORCA3 (CDS1)

    Plasmid
    #123185
    Purpose
    Golden Gate (MoClo; CDS1) compatible ORCA3 gene from Catharanthus roseus; an activator of the STR1 promoter from C. roseus
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    ORCA3 from Catharanthus roseus
    Use
    Part for plant expression
    Tags
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites
    Promoter
    Available since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123185-plasmid-map-sequence-id-241418

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  8. PGK-HA-PGRN-LAMP1TM

    Plasmid
    #234873
    Purpose
    Lentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion.
    Depositor
    Andrew Arrant
    Article
    Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi
    Insert
    Progranulin (GRN Human)
    Use
    Lentiviral
    Tags
    HA tag and LAMP-1 transmembrane domain and cytoso…
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    April 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    234873-plasmid-map-sequence-id-469050

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  10. pJL CusS NarX Chimera

    Plasmid
    #225652
    Purpose
    Expresses a NarX-based chimera capable of sensing copper (II). For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    CusS-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available since
    Dec. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    225652-plasmid-map-sequence-id-455039

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  11. pJL NrsS NarX Chimera

    Plasmid
    #225653
    Purpose
    Expresses a NarX-based chimera capable of sensing nickel (II). For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    NrsS-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225653-plasmid-map-sequence-id-454026

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  12. pJL RssA NarX Chimera

    Plasmid
    #225654
    Purpose
    Expresses a NarX-based chimera capable of sensing iron (III). For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    RssA-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225654-plasmid-map-sequence-id-454025

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  13. pJL VanS NarX Chimera

    Plasmid
    #225655
    Purpose
    Expresses a NarX-based chimera capable of sensing vancomycin. For use with NarL-YdfI and reporter plasmids from this study.
    Depositor
    Neha Kamat
    Article
    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120
    Insert
    VanS-NarX
    Use
    Cell free expression
    Tags
    Myc
    Expression
    Bacterial
    Mutation
    Transmembrane and sensing domain replaced to dete…
    Promoter
    T7
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225655-plasmid-map-sequence-id-454023

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  14. pDEINO1

    Plasmid
    #179472
    Purpose
    Multi-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugation
    Depositor
    Bogumil Karas
    Article
    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1
    Inserts
    ori
    cat
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    179472-plasmid-map-sequence-id-375147

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  16. pGWB14-LYX161

    Plasmid
    #126379
    Purpose
    With a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.
    Depositor
    Silin Zhong
    Insert
    Niben101Scf04395g00017.1
    Use
    Tags
    HA
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126379-plasmid-map-sequence-id-247568

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  17. mEos3.2trLATm2allL+A

    Plasmid
    #203750
    Purpose
    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    trLATm2(allL+A) (LAT Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203750-plasmid-map-sequence-id-403335

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  18. pGWB14-LYX024

    Plasmid
    #126242
    Purpose
    With a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.
    Depositor
    Silin Zhong
    Insert
    Niben101Scf03035g01006.1
    Use
    Tags
    HA
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    June 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    126242-plasmid-map-sequence-id-246333

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  19. pGWB14-LYX150

    Plasmid
    #126368
    Purpose
    With a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.
    Depositor
    Silin Zhong
    Insert
    Niben101Scf05177g03023.1
    Use
    Tags
    HA
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    June 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    126368-plasmid-map-sequence-id-247516

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  20. pGWB14-LYX149

    Plasmid
    #126367
    Purpose
    With a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.
    Depositor
    Silin Zhong
    Insert
    Niben101Scf01917g11015.1
    Use
    Tags
    HA
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    June 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    126367-plasmid-map-sequence-id-247515

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Showing: 721 - 740 of 52635 results