165,343 results

1. pCXLE-hMLN

   Plasmid

   #27079

   Purpose

   Non-integrating (episomal) expression of human C-MYC, LIN28 and NANOG

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Nat Methods. 2011 May;8(5):409-12

   Insert

   C-MYC, LIN28, NANOG (MYC Human)
   

   Expression

   Mammalian

   Available Since

   May 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSQT1601

   Plasmid

   #53369

   Purpose

   human codon optimized Csy4 and FokI-dCas9 expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   hCsy4-T2A-NLS-hFokI-dCas9-NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWZL Neo Myr Flag GRK5

   Plasmid

   #20495

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   GRK5 (GRK5 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFGC-pcoCas9

   Plasmid

   #52256

   Purpose

   A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

   Depositor

   Jen Sheen

   Insert

   35SPPDK:pcoCas9:NOS cassette
   

   Use

   CRISPR; Plant expression

   Promoter

   constitutive 35SPPDK promoter

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSC-NLS-RFP

   Plasmid

   #221813

   Purpose

   Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear localization sequence (NLS)

   Depositor

   Baojin Ding

   Article

   Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

   Insert

   NLS-mCherry
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

   Plasmid

   #199605

   Purpose

   Expression of cell permeable opAsCas12a in E. Coli.

   Depositor

   Junwei Shi

   Article

   Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   opCas12a-T8N
   

   Use

   CRISPR

   Tags

   6xHis, Twin-strep, SUMO

   Expression

   Bacterial

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti.CAG.H2B-cerFP.W.SINloxP

   Plasmid

   #51006

   Purpose

   Lentivector that expresses H2B-cerFP from internal CAG enhancer-promoter

   Depositor

   Rusty Lansford

   Insert

   H2B-cerFP
   

   Use

   Lentiviral

   Tags

   histone 2B

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. SIRT7 Flag

   Plasmid

   #13818

   Purpose

   Mammalian expression of human SIRT7 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT7 (SIRT7 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Missing exon 2

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. R777-E285 Hs.ROCK2

   Plasmid

   #70569

   Purpose

   Gateway ORF clone of human ROCK2 [NM_004850.3] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   ROCK2 (ROCK2 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Opie2-PuroR

    Plasmid

    #131613

    Purpose

    Expresses PuroR and dsRed.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Inserts

    PuroR
    dsRed
    

    Expression

    Insect

    Promoter

    Opie2 and hr5Ie1

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmCherry-RIAM

    Plasmid

    #80030

    Purpose

    Mammalian expression of mCherry-RIAM (FL)

    Depositor

    Chinten James Lim

    Insert

    RIAM (APBB1IP Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pZE21-GFPaav

    Plasmid

    #26643

    Depositor

    Michael Elowitz

    Article

    Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

    Insert

    pZE21-GFPaav
    

    Tags

    GFPaav

    Expression

    Bacterial

    Mutation

    na

    Available Since

    June 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104911

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nup133_mEGFP(-8a)

    Plasmid

    #163417

    Purpose

    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)

    Depositor

    Sanford Simon

    Insert

    Nup133 (NUP133 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMIIS1011

    Plasmid

    #238212

    Purpose

    Expresses ParTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.

    Depositor

    Feng Zhang

    Article

    Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

    Insert

    ParTasR
    

    Tags

    HA-NLS

    Expression

    Mammalian

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-EF1a-IRES-Puro GFP-3xFLAG

    Plasmid

    #201636

    Purpose

    Over-expression of 3xFLAG-tagged GFP

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. VPS13C^mClover3

    Plasmid

    #118760

    Purpose

    VPS13C internally tagged with mClover3 at residue 1914.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13C (VPS13C Human)
    

    Tags

    mClover3 inserted at position aa 1914

    Expression

    Mammalian

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRT043-CLYBL-DDdcas9-VPH

    Plasmid

    #158091

    Purpose

    Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

    Depositor

    Martin Kampmann

    Article

    Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

    Insert

    CLYBL-CAG-DDdCas9VPH-T2A-GFP
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. STIM1-EGFP

    Plasmid

    #210370

    Purpose

    Expresses STIM1 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    STIM1 (STIM1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. ALIX-mNeonGreen

    Plasmid

    #191191

    Purpose

    Full-length ALIX with C-terminal mNeonGreen tag.

    Depositor

    Jin Zhang

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX (PDCD6IP Human, Synthetic)
    

    Tags

    6xHIS and mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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