169,673 results

Showing: 7381 - 7400 of 169673 results

Results per page:

Sort results by:

pEGFPc3 K-RAS 4B

Plasmid

#236452

Purpose

transient overexpression of K-RAS 4B in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

K-RAS 4B (KRAS Human)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-sgRNA-BfuAI-2k

Plasmid

#112915

Purpose

Empty sgRNA expression plasmid

Depositor

Ren-Jang Lin

Article

Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CLYBL_Bxb1-GA_LP_Dual_TC

Plasmid

#229776

Purpose

CLYBL targeting construct with the Bxb1-GA landing pad cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

FRT-mScarlet-attP(GA)-PuroR-F3

Use

Synthetic Biology

Expression

Mammalian

Promoter

pGK

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-CMV-Nrf2

Plasmid

#67636

Purpose

AAV vector expressing Nrf2 gene

Depositor

Connie Cepko

Article

Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

Insert

Nrf2 (Nfe2l2 Mouse)

Use

AAV

Promoter

CMV

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX6P1-mCherry-Nanobody (LaM-2)

Plasmid

#162276

Purpose

Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

Insert

mCherry-Nanobody (LaM-2)

Tags

GST

Expression

Bacterial

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IZ-ANXA11-mRuby3

Plasmid

#184894

Purpose

Stable expression of ANXA11-mRuby3 (Annexin A11-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA11 (ANXA11 Human)

Tags

mRuby3

Expression

Mammalian

Mutation

R230C relative to NP_001148.1

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-DAPK1

Plasmid

#116727

Purpose

Lentiviral expression of DAPK1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

DAPK1 (DAPK1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas9-miniTurbo

Plasmid

#219822

Purpose

EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

Depositor

Philipp Maass

Insert

dCas9

Use

CRISPR

Tags

miniTurbo

Expression

Mammalian

Mutation

D10A/H841A

Promoter

EF1a

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pACEMam2-TRPML1

Plasmid

#238303

Purpose

Expression of human TRPML1 in mammalian cells

Depositor

Pravin Mahajan

Article

Reeks et al Structure. 2025 Aug 7;33(8):1374-1385.e7

Insert

TRPML1 (MCOLN1 Human)

Tags

8xHis-3xFLAG-TEV

Expression

Mammalian

Promoter

hybrid CAG promoter

Available Since

June 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pOpen-T7gene5

Plasmid

#165534

Purpose

DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

T7 DNA Polymerase (unmodified)

Use

Synthetic Biology

Available Since

Aug. 5, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
PEmax-mRNA

Plasmid

#204472

Purpose

plasmid for PEmax mRNA IVT

Depositor

Scot Wolfe

Article

Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456.

Insert

Prime editor

Use

CRISPR

Tags

no

Expression

Mammalian

Promoter

T7

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT311_pH11-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52544

Purpose

Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
mP58.FL1-pCDNA3

Plasmid

#21883

Depositor

David Ron

Article

Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

Insert

P58 (Dnajc3 Mouse)

Expression

Mammalian

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-fDIO-EYFP-WPRE

Plasmid

#154870

Purpose

Human synapsin-1 promoter; Flp-dependent EYFP

Depositor

Ulrik Gether

Insert

EYFP

Use

AAV and Mouse Targeting; Flp-frt

Expression

Mammalian

Promoter

Human synapsin promoter

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO-EGFP-WPRE-pA

Plasmid

#37084

Purpose

Cre-dependent expression of EGFP

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

EGFP

Use

AAV and Cre/Lox; Cre-on

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA3790

Plasmid

#70110

Purpose

mtDNA elimination/production of rho-0 cells

Depositor

Mikhail Alexeyev

Article

Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

Inserts

EGFP
hUNG1 Y147A
Neo

Tags

None, human OTC mitochondrial targeting sequence,…

Expression

Mammalian

Mutation

Y147A, aa's 1-77 removed

Promoter

CMV, EF1a, and SV40 and native

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458-sgTSC2

Plasmid

#128098

Purpose

CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

sgRNA against human TSC2 (TSC2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AAT-PB-CG2APtk

Plasmid

#86003

Purpose

homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

eGFP_T2A_puro_dTK

Use

Piggybac exciseable

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PFKFB3

Plasmid

#23668

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PFKFB3 (PFKFB3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-VEGFR2-V5/HIS

Plasmid

#205617

Purpose

Expression of human VEGFR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

VEGFR2 receptor tyrosine kinase (KDR Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge