159,371 results

1. pmcherry-LCa

   Plasmid

   #53972

   Purpose

   Expresses the light chain of clathrin

   Depositor

   Tom Kirchhausen

   Article

   Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

   Insert

   clathrin light chain
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

2. TFORF2603

   Plasmid

   #143373

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF471 (ZNF471 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-Sport6-CD63-pHluorin

   Plasmid

   #130901

   Purpose

   Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD63-pHluorin (CD63 Aequorea victoria, Human)
   

   Use

   Tags

   pHluorin

   Expression

   Mammalian

   Mutation

   pHluorin inserted between Gln-36 and Leu-37 in ex…

   Promoter

   CMV

   Available since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCWori cytochrome P450-BM3h 9D7

   Plasmid

   #61308

   Purpose

   Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

   Depositor

   Alan Jasanoff

   Article

   Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

   Insert

   Cytochrome P450-BM3 heme domain 9D7
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T268A, I263A, T438V, A328G

   Promoter

   Tac

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. ATG-2460

   Plasmid

   #108713

   Purpose

   EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)

   Depositor

   Keith Wood

   Article

   Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

   Insert

   CBR2opt-copGFP fusion
   

   Use

   Lentiviral

   Tags

   CBR2opt fused to copGFP

   Expression

   Mutation

   Promega used directed evolution to create CBR (Cl…

   Promoter

   elongation factor-1

   Available since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOCC5

   Plasmid

   #118863

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-puro /BRAF-V600E

   Plasmid

   #131724

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

   Insert

   BRAF (BRAF Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   V600E

   Promoter

   CMV

   Available since

   Feb. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMRX-No-HaloTag7-mGFP-LC3

   Plasmid

   #184901

   Purpose

   Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Use

   Tags

   HaloTag7 and monomeric EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS410

   Plasmid

   #11258

   Depositor

   Fred Cross

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLminP_Luc2P_RE60

    Plasmid

    #90405

    Purpose

    STAT5 - Stat5 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SIE

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe-Kras Wt

    Plasmid

    #75282

    Purpose

    Expresses human Kras wild-type

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    None. Wild-Type

    Promoter

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTALYM3B15

    Plasmid

    #47878

    Purpose

    TALE-mClover against mouse major satellite sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mClover
    

    Use

    TALEN

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF2593

    Plasmid

    #142336

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BHLHE40 (BHLHE40 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pATZ-Myc

    Plasmid

    #177718

    Purpose

    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant), myc tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Use

    Tags

    Myc

    Expression

    Bacterial and Mammalian

    Mutation

    E342K (ATZ mutant)

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGWB502-omega

    Plasmid

    #74845

    Purpose

    Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2xCaMV35S enhancer and omega

    Available since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF0040

    Plasmid

    #143684

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF676 (ZNF676 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHCMV_DUGV_GP

    Plasmid

    #207268

    Purpose

    Mammalian expression of Dugbe virus GP protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    DUGV_GP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. mT-Sapphire-N1

    Plasmid

    #54569

    Purpose

    Localization: N1 Cloning Vector, Excitation: 399, Emission: 511

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Ai et al Nat Methods. 2008 May . 5(5):401-3.

    Type

    Empty backbone

    Use

    Tags

    mT

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pEvoltac-EcW-TGA-h14

    Plasmid

    #218764

    Purpose

    encodes EcWRS-h14 and EcW-tRNA-TGA

    Depositor

    Abhishek Chatterjee

    Article

    Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

    Insert

    EcWRS-h14
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S8A, V144G, V146C

    Promoter

    tacI

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSCAR_sgRNA_hygro-tagBFP-lox5171

    Plasmid

    #162070

    Purpose

    3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

    Depositor

    Robert Manguso

    Article

    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge