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scFv-sfGFP-DNMT3A1

Plasmid

#102278

Purpose

The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

Depositor

Margaret Goodell

Publication

Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

Insert

DNMT3A1 (DNMT3A Human)

Use

Lentiviral

Tags

scFV-sfGFP

Expression

Mammalian

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pPB-CAG-rtTA-IRES-Hygro

Plasmid

#102423

Purpose

Mammalian expression vector for tetracycline-transactivator (rtTA), IRES-Hygro

Depositor

Kian Peng Koh

Publication

Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tetracyclin-transactivator (rtTA)

Expression

Mammalian

Promoter

CAG

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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MCP-YFP

Plasmid

#101160

Purpose

to express MCP-YFP fusion protein

Depositor

Mazhar Adli

Publication

Qin et al Nat Commun. 2017 Mar 14;8:14725. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MCP-YFP

Expression

Mammalian

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GFP-mROCK2

Plasmid

#101296

Purpose

Mammalian expression of Rock2-GFP

Depositor

Alpha Yap

Publication

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rock2 (Rock2 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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p7056 pHAGE-P-CMVt-N-HA-GAW-CPT1A

Plasmid

#100146

Purpose

Expresses CPT1A

Depositor

Peter Howley

Publication

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

CPT1A (CPT1A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCW57.1-DUX4-CA

Plasmid

#99281

Purpose

All-in-one lentivector with tet-inducible DUX4 (codon sequences altered)

Depositor

Stephen Tapscott

Publication

Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

Insert

DUX4 (codon altered) (DUX4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pDIRECT_23C

Plasmid

#91140

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

Plasmid

#86230

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Publication

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

AID[71-114]-eGFP

Use

Mouse Targeting

Expression

Mammalian

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFC333

Plasmid

#87844

Purpose

AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

Depositor

Uffe Mortensen

Publication

Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

Inserts

Cas9
ble (bleomycin resistance marker)

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Promoter

Aspergillus nidulans tef1 promoter and Aspergillu…

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pT7-VP7SA11

Plasmid

#89167

Purpose

Express Rotavirus SA11 VP7 in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rotavirus SA11 VP7

Tags

*see below

Expression

Mammalian

Promoter

T7 promotor

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR223_FBXW7_WT

Plasmid

#81795

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_BRAF_p.V600E

Plasmid

#81700

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway Cloning

Mutation

V600E

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223_KRAS_p.G12D

Plasmid

#81651

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

G12D

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pT7-NSP5SA11

Plasmid

#89172

Purpose

Express Rotavirus SA11 NSP5 in mammalian cells

Depositor

Takeshi Kobayashi

Publication

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rotavirus SA11 NSP5

Expression

Mammalian

Promoter

T7 promotor

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1_miniSOG2 T2A H2B-EGFP

Plasmid

#87410

Purpose

Mammalian expression vector encoding Histone 2B GFP fusion and miniSOG2

Depositor

Xiaokun Shu

Publication

Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mini singlet oxygen generator 2

Expression

Mammalian

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRN111

Plasmid

#84463

Purpose

pJB38-NWMN29-30 + SarA_P1-mCherry-Term

Depositor

Reindert Nijland

Publication

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SarA_P1-mCherry from pRN11

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast

Plasmid

#85146

Purpose

lentiviral expression of a stoichiometric F-actin and myosin light chain reporter

Depositor

Tobias Meyer

Publication

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Ftractin-mRuby3-P2A-mTurquoise-MLC

Use

Lentiviral

Tags

mRuby3, mTurquoise

Promoter

EF1a

Available since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pAAV-hSyn-FLEX-TVA-P2A-EGFP-2A-oG

Plasmid

#85225

Purpose

Expresses TVA, EGFP, and optimized Rabies G (oG) protein in a FLEX cassette

Depositor

John Naughton

Inserts

Optimized G Protein
Enhanced GFP
TVA (CD320 Chicken)

Use

AAV and Cre/Lox

Promoter

hSyn

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

Plasmid

#83485

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

Depositor

Jennifer Doudna

Publication

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A, R1048A, H1053A

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCherry-GFP1-10

Plasmid

#78591

Purpose

Express mCherry-GFP1-10 in mammalian cells

Depositor

Yihong Ye

Publication

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP 1-10

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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