19,660 results

1. pJBEI-6409

   Plasmid

   #47048

   Purpose

   BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

   Depositor

   Taek Soon Lee

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Insert

   Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
   

   Use

   Synthetic Biology; Bglbrick

   Expression

   Bacterial

   Promoter

   PlacUV5

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-PFKFB3

   Plasmid

   #23668

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PFKFB3 (PFKFB3 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. PKC alpha WT

   Plasmid

   #21232

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC alpha (PRKCA Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRES2_EGFP_KCNQ1

   Plasmid

   #173161

   Purpose

   For mammalian expression of KCNQ1 and creating variants

   Depositor

   Al George

   Article

   Vanoye et al Circ Genom Precis Med. 2018 Nov;11(11):e

   Insert

   KCNQ1 (KCNQ1 Human)
   

   Tags

   IRES2-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWZL Blast DN E-cadherin

   Plasmid

   #18800

   Depositor

   Bob Weinberg

   Article

   Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

   Insert

   Dom neg E-cadherin (Cdh1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   truncated form of E-cadherin (dominant negative) …

   Available Since

   July 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-teLuc c-myc

   Plasmid

   #100026

   Purpose

   Enhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)

   Depositor

   Huiwang Ai

   Article

   Yeh et al Nat Methods. 2017 Sep 4. doi: 10.1038/nm

   Insert

   teLuc, a NanoLuc mutant
   

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   D19S, D85N, C164H

   Promoter

   CMV

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 H2B-mIFP T2A Mpro (wt)

   Plasmid

   #163079

   Purpose

   Expresses wild type Mpro (active) and a near-infrared fluorescent protein mIFP fused to H2B

   Depositor

   Xiaokun Shu

   Insert

   Wild type Mpro of SARS-CoV-2 and H2B-mIFP
   

   Expression

   Mammalian

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ1661-sgMUC4-E3(F+E)

   Plasmid

   #51025

   Purpose

   Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3

   Depositor

   Bo Huang, Stanley Qi

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   optimized sgRNA (MUC4 Mouse, Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. WT GLUT1-GFP

   Plasmid

   #200093

   Purpose

   WT human GLUT1 with a C-terminal GFP tag in pQCXIP vector

   Depositor

   Jason MacGurn

   Article

   Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

   Insert

   GLUT1 (SLC2A1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti PGK Blast V5-LUC (w528-1)

    Plasmid

    #19166

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Blast

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHCMV-SS-antiHAscFv-TM

    Plasmid

    #198302

    Purpose

    Displays anti-HA scFv from the surface of mammalian cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pHCMV-SS-antiHAscFv-TM
    

    Expression

    Mammalian

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCGN-ELK-1

    Plasmid

    #27156

    Purpose

    For mammalian expression of human Elk-1 with an HA tag

    Depositor

    Ron Prywes

    Article

    Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

    Insert

    ELK1 (ELK1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Dec. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBiFC-VN155(I152L)

    Plasmid

    #27097

    Depositor

    Chang-Deng Hu

    Article

    Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

    Insert

    VN155(I152L)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    I152L

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCrispr-V2-mOrange (V2mO)

    Plasmid

    #140206

    Purpose

    Lenti Crispr/Cas9 with mOrange fluorescent marker

    Depositor

    Jaffer Ajani

    Article

    Jin et al PLoS One. 2020 Feb 13;15(2):e0228910. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    pELF1a

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIS0

    Plasmid

    #12178

    Purpose

    3'UTR segments of target genes can be inserted into this firefly luciferase reporter to test for their effects on protein production

    Depositor

    David Bartel

    Article

    Yekta et al Science. 2004 Apr 23. 304(5670):594-6.

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-WHaloCaMP1a-EGFP

    Plasmid

    #205313

    Purpose

    Expression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a-EGFP
    

    Expression

    Mammalian

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid

    #87828

    Purpose

    High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Promoter

    CMV promoter, tetracycline operator

    Available Since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMJ114

    Plasmid

    #85995

    Purpose

    One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    modified bovine U6-2

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHW394 (15xUAS::GFP::let-858 3'UTR)

    Plasmid

    #85584

    Purpose

    cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

    Depositor

    Paul Sternberg

    Article

    Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Insert

    15xUAS-delta pes-10-GFP
    

    Expression

    Worm

    Mutation

    GFP(S65C) with three introns for expression in C.…

    Promoter

    15xUAS-delta pes-10-GFP

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAT001

    Plasmid

    #171061

    Purpose

    Plasmid for expressing HIV-1 envelope glycoprotein

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Insert

    HIV-1 Env
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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