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Plasmid#204748PurposeExpression of Cas9 and human H2AX with the S139D mutationDepositorInsertH2AX S139D (H2AX Human)
UseTagsExpressionMammalianMutationH2AX with the S139D mutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_13
Plasmid#194674PurposeProtein production of the split HaloTag based calcium recorder Caprola_13 in bacteriaDepositorInsertCaprola_13
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHA1.1-Rap2a-V5-Hygro
Plasmid#195510Purposelentiviral vector expressing RAP2A CDS with hygromycin selectionDepositorInsertHygromycin
UseLentiviralTagsExpressionMammalianMutationPromoterhPGKAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-yeGFP-His6
Plasmid#212781PurposeC-terminal PCR-based tagging with yeGFP-His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDL17_Cas9-His Δcys M1C
Plasmid#206251PurposeBacterial expression of SpCas9 Δcys M1C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationM1C, C80S, C574SPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-sCAG-eGFP-ACAP1 F280E
Plasmid#203801PurposeAAV vector plasmid expressing human ACAP1 mutation F280E fused to eGFP under a short variant of the CAG (sCAG) promoterDepositorInsertACAP1 (ACAP1 Human)
UseAAVTagseGFPExpressionMammalianMutationF280EPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
HisTEV-cDAXX
Plasmid#197012PurposeE. coli expression vectorDepositorInsertDAXX
UseTags6His-TEVExpressionBacterialMutationPromoterAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-PBZC-Linker-eGFP
Plasmid#176070PurposeEGFP fused to the C-terminus of a PBZ domain & a hygromycin resistance cassetteDepositorInsertPBZ-C
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-PBM-Linker-eGFP
Plasmid#176069PurposeEGFP fused to the C-terminus of a PBM domain & a hygromycin resistance cassetteDepositorInsertPBM
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCaMKII-EBFP-tTAn
Plasmid#172124PurposeExpression of EBFP and tTA-InteinN in CaMKII positive cellsDepositorInsertpCaMKII, EBFP, tTAn-InteinN
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pCaMKII-tTAc
Plasmid#172125PurposeExpression of InteinC-tTAC in CaMKII positive cellsDepositorInsertpCaMKII, tTAc
UseAAVTagsExpressionMutationPromoterCaMKIIAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
His Puc (aa 1-393)
Plasmid#52295PurposeBacterial expression of His tagged Drosophila Puc fragmentDepositorInsertPuc (puc Fly)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gs(short)-CASE
Plasmid#168124PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).DepositorUseLuciferaseTagscpVenus on GNG1ExpressionMammalianMutationNLuc is inserted at N136/V137 within GNASPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRDA_078
Plasmid#158582PurposeLentiviral expression of BE4max and customizable gRNADepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationCas9 D10APromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUC01
Plasmid#177204Purposechromogenic reporter for gene knockout in StreptomycesDepositorInsertSaindC
UseSynthetic BiologyTagsNONEExpressionBacterialMutationNONEPromoterTGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…Available sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN77
Plasmid#61347PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/IkBα NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and NES IkBaExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS314-4M5.3
Plasmid#45830DepositorInsert4M5.3
UseTagsFLAGExpressionYeastMutationPromoterGAL1Available sinceJune 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pST-KT
Plasmid#44561Purposefor ectopic inducible expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialMutationPromoterUV15 promoterAvailable sinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
7a sgRNA for EJ7-GFP and 4-μHOM reporters
Plasmid#113620PurposesgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reportersDepositorInsert7a sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
POLG-pcDNA3.1
Plasmid#191487PurposeExpresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.DepositorInsertPOLG (POLG Human)
UseTagsV5 tag, 6X HisExpressionMammalianMutationPromoterCMV, T7Available sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB512
Plasmid#74854PurposeGateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF0702
Plasmid#144361PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNCOR2 (NCOR2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (1-517, WT, MG-38-11)
Plasmid#110748PurposeTransient transfection of human USP30DepositorInsertUSP30 (USP30 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCM433
Plasmid#15670DepositorTypeEmpty backboneUseBacterial allelic exchange vectorTagsExpressionMutationPromoterAvailable sinceAug. 31, 2007AvailabilityIndustry, Academic Institutions, and Nonprofits -
Flag-HA-USP53
Plasmid#22606DepositorInsertUSP53 (USP53 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationnot FL, missing first 65 aaPromoterCMV/TOAvailable sinceNov. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3. Flag-CSL R192H
Plasmid#167005PurposeMammalian expression of CSL R192HDepositorInsertCSL (RBPJ Human)
UseTagsFLAGExpressionMammalianMutationR192HPromoterCMVAvailable sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST
Plasmid#174007PurposeCre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the control of an hSyn promoter. jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A.DepositorHas ServiceAAV9InsertjGCaMP8s-P2A-ChrimsonR-ST
UseAAVTags6xHis and soma targeting motifExpressionMutationPromoterhSynAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv6_CBX7_2xChromo
Plasmid#179396PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX7_2xChromoDepositorInsertCBX7_2xChromo
UseTagsAviTag and EGFPExpressionMammalianMutationG113V in flexible linkerPromoterCAGGSAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
VCP(R155H)-EGFP
Plasmid#23972DepositorInsertValosin containing protein (VCP Human)
UseTags6xHis, EGFP, and HAExpressionMammalianMutationR155HPromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 mRuby
Plasmid#216225PurposeTDP-43 with a C-terminal mRuby tag for expression in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseTagsmRubyExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only