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pBbS8k-RFP_N99D

Plasmid

#109410

Purpose

RepA Mutant

Depositor

Jay Keasling

Article

Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

Insert

repA

Tags

RFP

Expression

Bacterial

Mutation

N99D repA mutation to increase copy number

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-Rps7-meGFP

Plasmid

#241318

Purpose

Mouse Rps7 fused to meGFP for expression in mammalian cells

Depositor

Stavros Lomvardas

Insert

Rps7 (Rps7 Mouse)

Tags

meGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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TLCV2 - sgPTEN

Plasmid

#202759

Purpose

Encodes Doxycycline inducible Cas9 and sgRNA targeting PTEN

Depositor

Christopher Counter

Article

Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

Insert

gRNA targeting PTEN

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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human p53-(1-320)

Plasmid

#24864

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-320

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
EGFP-talin1 head

Plasmid

#32856

Depositor

Anna Huttenlocher

Article

Simonson et al J Immunol. 2006 Dec 1;177(11):7707-14.

Insert

Talin-1 Head aa 1-433 (Tln1 Mouse)

Tags

Enhanced GFP

Expression

Mammalian

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry-LacZshRNA

Plasmid

#73978

Purpose

LacZshRNA (mir155 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the LacZ gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHO-UAS-mScarlet-I

Plasmid

#183147

Purpose

Yeat two-hybrid fluorecent reporter for genomic integration

Depositor

Assaf Mosquna

Article

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

mScarlet-I

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGY208

Plasmid

#239803

Purpose

Express mCherry in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

mCherry

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-CMVSP6

Plasmid

#138356

Purpose

Gateway compatible 5' entry clone contains Cytomegalovirus and SP6 dual promoter(CMVSSP6)

Depositor

Nathan Lawson

Article

Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

Insert

CMV-SP6 promoter

Use

Gateway compatible

Promoter

CMV-SP6

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pVTU_260_BirA

Plasmid

#63682

Purpose

Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

Depositor

Aaron Hoskins

Article

Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

Insert

BirA

Tags

6X Histidine and TEV cleavage site

Expression

Yeast

Promoter

ADH1/yeast

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_eGFP_TIAL1

Plasmid

#106091

Purpose

gene expression of EGFP-TIAL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TIAL1 (TIAL1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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TOPO mCherry

Plasmid

#68490

Purpose

TOPO construct expressing mCherry for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mCherry

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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WT-Pan

Plasmid

#99638

Purpose

EGFP-mCherry control reporter for methionine misincorporation

Depositor

Tao Pan

Article

Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

Insert

mCherry

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pKDR-EF1a

Plasmid

#183567

Purpose

One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated EF1a promoter)

Depositor

Erik Dent

Article

Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

Insert

Unattenuated

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-5

Plasmid

#124985

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-5 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-5 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA-myc-HisA-Sirt3

Plasmid

#24924

Depositor

Toren Finkel

Article

Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

Insert

Sirt3 (SIRT3 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pCL-MFG-BRCA1

Plasmid

#12341

Depositor

Inder Verma

Article

Ruffner et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

Insert

BRCA1 (BRCA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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M-NM-sgRNA

Plasmid

#48673

Purpose

Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter

Use

CRISPR

Promoter

hU6

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-PHLPP1 N-terminal truncation

Plasmid

#22404

Purpose

Expresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cells

Depositor

Alexandra Newton

Article

Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

Insert

PHLPP1 (PHLPP1 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-mCherry-kanMX6

Plasmid

#89068

Purpose

genomic GBP-mCherry epitope C-terminal tagging in S. pombe

Depositor

Quanwen Jin

Article

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

GBP-mCherry

Expression

Yeast

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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RhoG

Plasmid

#55694

Purpose

expression of mouse RhoG

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoG (Rhog Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pH6HTC_CCDC124-Halo

Plasmid

#175328

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCDC124 (CCDC124 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-4

Plasmid

#124984

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-4 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-4 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2_8xCTS-ECFP

Plasmid

#200249

Purpose

Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

8xCTS-ECFP

Use

Zebrafish expression

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRL25

Plasmid

#70253

Purpose

Shuttle vector suitable for transfer to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains

Depositor

Peter Wolk

Article

Wolk et al J Bacteriol. 1988 Mar;170(3):1239-44.

Type

Empty backbone

Use

Bacterial shuttle vector

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH-F-PA1

Plasmid

#40618

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

PA1 (PAGR1 Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-MKRN1

Plasmid

#78745

Purpose

To overexpress MKRN1 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pJAM1.16_PB-Cx43_S368A-PGK-Blast

Plasmid

#205561

Purpose

Express human Cx43 with S368A mutation

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Cx43

Use

Piggybac

Expression

Mammalian

Mutation

S368A

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_Tmcs

Plasmid

#25751

Purpose

Entry vector with TRE promoter followed by multiple cloning site.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-RFP-kanMX6

Plasmid

#105160

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-kanMX6

Tags

GBP-RFP

Expression

Bacterial and Yeast

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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