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R4pGWB601_UBQ10p-miniTurbo-NES-YFP

Plasmid

#127369

Purpose

Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Tags

GS linker, NES, V5, and YFP

Expression

Plant

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

Arabidopsis UBQ10 promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP1

Plasmid

#129504

Purpose

Expresses AvicFP1 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP1

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP2

Plasmid

#129505

Purpose

Expresses AvicFP2 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP2

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pNCST-AvicFP3

Plasmid

#129506

Purpose

Expresses AvicFP3 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AvicFP3

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD1

Plasmid

#195839

Purpose

Expresses human FZD1 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD1 (FZD1 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgNUAK2

Plasmid

#138693

Purpose

Expresses a human NUAK2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNUAK2 human (NUAK2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSB117 - pL2_pSB90_fLUC-I_V2

Plasmid

#123192

Purpose

binary plant vector for transient TYLCV V2 and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::V2::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD10

Plasmid

#195848

Purpose

Expresses human FZD10 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD10 (FZD10 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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Arf6 T27N- Venus in pcDNA3.1

Plasmid

#90464

Purpose

RhoA mediated cell migration

Depositor

Narasimhan Gautam

Article

Castillo-Badillo et al ACS Synth Biol. 2020 Mar 16. doi: 10.102

Insert

Arf6 T27N (ARF6 )

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn1 YFP-CaRhAC

Plasmid

#101721

Purpose

Yellow fluorescent protein (YFP) – tagged rhodopsin adenylyl cyclase created from the rhodopsin guanylyl cyclase of Catenaria anguillulae with a neuron-specific promoter. Useful for raising intracellu

Depositor

Thomas Oertner

Article

Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

Insert

YFP CaRhAC

Use

AAV

Tags

YFP

Expression

Mammalian

Promoter

hSyn1

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD5

Plasmid

#195843

Purpose

Expresses human FZD5 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD5 (FZD5 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSB116 - pL2_pSB90_fLUC-I_P19

Plasmid

#123191

Purpose

binary plant vector for transient TBSV P19 and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::P19::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pNCST-mAvicFP1

Plasmid

#129509

Purpose

Expresses mAvicFP1 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

mAvicFP1

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSB118 - pL2_pSB90_fLUC-I_HcPro

Plasmid

#123193

Purpose

binary plant vector for transient TEV HcPro and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::HcPro::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-VGAT-WPRE-pA

Plasmid

#39320

Depositor

Bernardo Sabatini

Article

Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

Insert

Slc32a1 (Slc32a1 Mouse)

Use

AAV; R

Available Since

Oct. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pNCST-AausFP2

Plasmid

#129500

Purpose

Expresses AausFP2 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP2

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pROF141

Plasmid

#155329

Purpose

Binary vector for expression of the PULSE system under the control of PAtUbi10 promoter

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_PAtUbi10-SRDX-NLS-EL222-Tnos_SF_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR4 PARP2 E614Q

Plasmid

#192545

Purpose

pENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

poly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)

Use

Gateway entry vector

Mutation

changed Glu 614 to Gln

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pNCST-AausFP3

Plasmid

#129501

Purpose

Expresses AausFP3 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP3

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry PGK-1 in pcDNA3.1

Plasmid

#64214

Purpose

mCherry fused between Cry2 and PGK-1 to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
PGK1 (PGK1 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
pSB115 - pL2_pSB90_fLUC-I_tGFP-I

Plasmid

#123190

Purpose

binary plant vector for transient tGFP (with intron) and fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-Dicer-Y926F/R927A (3'-pocket mutant)

Plasmid

#51369

Depositor

Narry Kim

Article

Park et al Nature. 2011 Jul 13;475(7355):201-5. doi

Insert

Dicer (DICER1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

3' pocket mutant (Y926F, R927A)

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pNCST-AausFP4

Plasmid

#129502

Purpose

Expresses AausFP4 constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausFP4

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS

Plasmid

#123188

Purpose

binary plant vector for transient fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-CH115gp160-QES.c14-754*

Plasmid

#123282

Purpose

Mammalian expression plasmid for Env from the CH115 HIV-1 isolate; C-terminal truncation; QES mutant for enhanced presentation of quaternary epitopes

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (CH115) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-Dicer-5'-pocket mutant

Plasmid

#51368

Depositor

Narry Kim

Article

Park et al Nature. 2011 Jul 13;475(7355):201-5. doi

Insert

Dicer (DICER1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

5' pocket mutant (R778A, R780A, R811A, H982A…

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pEBG-MKK4-K129R (GST)

Plasmid

#21564

Depositor

Leonard Zon

Article

Sanchez et al Nature. . 372(6508):794-8.

Insert

mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)

Tags

GST

Expression

Mammalian

Mutation

Lysine 129 to arginine

Available Since

July 17, 2009

Availability

Academic Institutions and Nonprofits only
pGL3-CMV-Cdc42 3'UTR mutant (position 577-599)

Plasmid

#51391

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

NM_001039802.1 (CDC42 Human)

Use

Luciferase

Tags

Luciferase

Mutation

miR-29 binding site mutant (position 577-599)

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pLV-Hygro-EF1A-TRIP12(749-836)-EGFP

Plasmid

#196229

Purpose

EGFP fused to the C-terminus of a WWE domain within TRIP12 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged TRIP12(749- 836)

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-DTX2(8-174)-EGFP

Plasmid

#196232

Purpose

EGFP fused to the C-terminus of tandem WWE domains within Deltex2 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX2(8-174)-EGFP

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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