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pGEMHE:hTRAAK(Q258K)

Plasmid

#224763

Purpose

In vitro transcription for Oocyte expression of hTRAAK(Q258K)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Mutation

Q258K

Promoter

T7

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1

Plasmid

#224743

Purpose

In vitro transcription for Oocyte expression of mTREK-1

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Promoter

T7

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK

Plasmid

#224761

Purpose

In vitro transcription for Oocyte expression of hTRAAK

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:hTRAAK(S118C)

Plasmid

#224762

Purpose

In vitro transcription for Oocyte expression of hTRAAK(S118C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (KCNK4 Human)

Use

In vitro transcription for oocyte expression

Mutation

S118C

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTRAAK

Plasmid

#224765

Purpose

In vitro transcription for Oocyte expression of mTRAAK

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 4 (Kcnk4 Mouse)

Use

In vitro transcription for oocyte expression

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pPicZ:mTREK-1_cryst_I110D

Plasmid

#209640

Purpose

P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322, I110D) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Pope et al Cell Chem Biol. 2020 May 21;27(5):511-52

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) I110D mutant

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

Plasmid

#223163

Purpose

2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast coUCK2 D62A

Plasmid

#211530

Purpose

Expresses codon optimized catalytic mutant UCK2

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

UCK2 D62A (UCK2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

D62A (catalytic mutant)

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-hWIPI2B-3×FLAG

Plasmid

#215510

Purpose

Expresses FLAG tagged human WIPI2B.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 2B (WIPI2 Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-EGFP

Plasmid

#210378

Purpose

Expresses SARS-CoV-2 NSP4 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

NSP4 (ORF1ab SARS-CoV-2)

Tags

EGFP

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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NSP2-Flag

Plasmid

#210344

Purpose

Expresses SARS-CoV-2 NSP2 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP2 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-bII spectrin (repeats 13-15)

Plasmid

#213729

Purpose

Mammalian cell expression vector for overexpression of bII spectrin (repeats 13-15) in NIH/3T3 cells.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

bII spectrin (repeats 13-15) (Spnb3 Mouse)

Expression

Mammalian

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Blast

Plasmid

#214014

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Neo-TNNT2-Hygro

Plasmid

#214015

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Blast-TNNT2-Zeo

Plasmid

#214018

Purpose

Expression of cardiac troponin T

Depositor

Paul Burridge

Article

Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

Insert

TNNT2 (TNNT2 Human)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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SUMO-Twinkle 43-684

Plasmid

#205056

Purpose

TWNK protein expression

Depositor

Smita Patel

Article

Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

Insert

TWNK (TWNK Human)

Expression

Bacterial

Mutation

Lacks amino acid from 1-42

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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NSP3C-mCherry

Plasmid

#210377

Purpose

Expresses the C terminal of SARS-CoV-2 NSP3 fused with mCherry tag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

NSP3C (ORF1ab SARS-CoV-2)

Tags

mCherry

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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SEC61B-2Strep

Plasmid

#210375

Purpose

Expresses SEC61B fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SEC61B (SEC61B Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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TMEM106B-2Strep

Plasmid

#210360

Purpose

Expresses TMEM106B fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

TMEM106B (TMEM106B Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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SUMO-Twinkle 360-684

Plasmid

#205055

Purpose

TWNK protein expression

Depositor

Smita Patel

Article

Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

Insert

TWNK (TWNK Human)

Expression

Bacterial

Mutation

Lacks N terminal amino acid from 1-359

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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