We narrowed to 10,006 results for: Gnas
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Plasmid#198038PurposeExpression plasmid for producing SpyTagged stalled secretion variant of intiminDepositorInserteaeA
TagsSpyTagExpressionBacterialMutationlacks native signal peptide (amino acids 1-39)PromotertetAvailable SinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_ssSBP-EGFP
Plasmid#65277Purposeto synchronize transport of secretory soluble GFP from the ER (RUSH system)DepositorInsertEGFP
TagsIL-2 signal sequence and Streptavidin Binding Pr…ExpressionMammalianPromoterCMVAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-RSV-tdTomato-2A-GCaMP6s
Plasmid#80316PurposeFluorescent reporter for calcium signalingDepositorInsertstdTomato
GCaMP6s
UseLentiviralPromoterRSVAvailable SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a MDHP_YEAST
Plasmid#204233PurposeBacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHP_YEAST (MDH3 Budding Yeast)
Tags6X HisExpressionBacterialMutationThe last 3 C-terminal residues (SKL) are the pero…PromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEFIRES-P-mTagBFP-KDEL
Plasmid#87163PurposeFluorescent marker of endoplasmic reticulum (ER)DepositorInsertmTagBFP-KDEL
TagsBip signal peptide (Homo sapiens) and mTagBFP-KDE…ExpressionMammalianPromoterEF-1 alphaAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lyn-R-GECO1
Plasmid#120410PurposeExpresses a fusion between myristoylation signal peptide and R-GECO1. This construct targets R-GECO1 to the plasma membrane.DepositorInsertLyn-R-GECO1
ExpressionMammalianPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMIII-Neo
Plasmid#153422PurposeMSCV with IRES-Neomycin resistant geneDepositorTypeEmpty backboneUseRetroviralExpressionMammalianAvailable SinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-RSV-tdTomato-2A-GCaMP6f
Plasmid#80317PurposeFluorescent reporter for calcium signalingDepositorInsertstdTomato
GCaMP6f
UseLentiviralPromoterRSVAvailable SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
SOX2se-CPG-TV
Plasmid#70155Purposea targeting vector to target the endogenous Sox2 SE CPG island with Snrp-Tomato-polyADepositorInsert5'hSOX2se-Snrp-Tomato-polyA-Pgk-PURO-3'hSOX2se
UseMouse Targeting and Synthetic BiologyAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
tTA_BFP
Plasmid#58854PurposeConstitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)DepositorInserttTA transcription factor fused to enhanced blue fluorescent protein
TagsBFPExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)
Plasmid#70745Purposefor building 3-color reporter plasmidDepositorInsertmCherry-ssrA
Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJKR-O-mphR
Plasmid#62570PurposeMacrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.DepositorInsertmCherry
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNR291
Plasmid#79555PurposeOutput plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.DepositorInsertsGFPmut3b
mTagBFP
mRFP
ExpressionBacterialAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-PTS1-IRES-Puromycin
Plasmid#134665PurposeExpresses EGFP-tagged peroxisome markerDepositorInserttripeptide SKL (Peroxisome targeting signal 1)
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1aAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22b - PA
Plasmid#84863Purposebacterial expression of Bacillus anthracis protective antigenDepositorInsertPA
TagspelB signal sequenceExpressionBacterialPromoterT7Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-mCherry-NLS
Plasmid#194049PurposeTransient expression of mCherry-NLS in plant cell (Nucleus)DepositorInsertmCherry
TagsSV40 (Nuclear localization signal)ExpressionPlantPromoterCaMV 35S promoterAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBI-MCS-EYFP
Plasmid#58855PurposeThe tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFPDepositorInsertenhanced yellow fluorescent protein
ExpressionMammalianPromoterCMVAvailable SinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_SBP-EGFP-Ecadherin
Plasmid#65288Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorInsertEcadherin
TagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianPromoterCMVAvailable SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-3C-sGFP1-10_pcDNA3.1(-)
Plasmid#182931PurposeFor expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragmentDepositorTypeEmpty backboneTags3C-cleavable split GFP 1-10, FLAG tag, and HA sig…ExpressionMammalianPromoterCMVAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalPromoterTgTUB1 and Toxoplasma U6Available SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only