We narrowed to 17,810 results for: SHE

1. pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE)

   Plasmid

   #214880

   Purpose

   Lentiviral vector encoding RfxCas13d targeting CD46 guide

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   hU6-crCD46-EFS-PuroR-WPRE
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHD-SPARC2-D-LexA::p65

   Plasmid

   #133562

   Purpose

   Swap out effector and terminator to generate SPARC2-D CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   LexA::p65
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   20X UAS

   Available Since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. single polypeptide FPX biosensor for caspase-1

   Plasmid

   #60885

   Purpose

   FPX biosensor for caspase-1

   Depositor

   Robert Campbell

   Article

   Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

   Insert

   single polypeptide FPX biosensor for caspase-1
   

   Tags

   HindIII site and NES sequence LALKLAGLDIGS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCAGEN-WPRE-bGH-DEST (JDW 1309)

   Plasmid

   #229815

   Purpose

   A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-mCherry-LacR-BRCA1

   Plasmid

   #71115

   Purpose

   mammalian expression of mCherry-LacR-BRCA1 siRNA resistant construct

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   BRCA1 (BRCA1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV/TO

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHD-SPARC2-I-LexA::p65

   Plasmid

   #133563

   Purpose

   Swap out effector and terminator to generate SPARC2-I CRISPR-donor vector for CRISPR-HDR genomic insertion near the attP40 locus

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   LexA::p65
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   20X UAS

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_APOBEC1-UdgX-NG-nCas9

   Plasmid

   #163533

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   APOBEC1-UdgX-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_UdgX-EE-UdgX-nCas9-UdgX

   Plasmid

   #163566

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-nCas9-UdgX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTargetF-FRT

   Plasmid

   #113637

   Purpose

   Derivative of pTargetF with gRNA targeting FRT site

   Depositor

   Olivier Lichtarge

   Article

   Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

   Insert

   sgRNA-FRT
   

   Use

   CRISPR

   Promoter

   pJ23119

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. YES1 Y537F

    Plasmid

    #51299

    Purpose

    Expression of YES1 Y537F

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YES1 (YES1 Human)
    

    Use

    Lentiviral

    Mutation

    Y537F

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

    Plasmid

    #163543

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    POLD2-APOBEC1-UdgX-nCas9-UdgX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CaMKIIa-eArchT 3.0-EYFP

    Plasmid

    #35513

    Purpose

    Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    eArchT 3.0-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCas9-Venus

    Plasmid

    #70267

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    Cas9
    Venus Reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS and P2A

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV_Efs_hSpCas9_NLS_FLAG-SV40

    Plasmid

    #97307

    Purpose

    AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    humanized S. pyogenes Cas9
    

    Use

    AAV and Mouse Targeting

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PD-1-miSFIT-6-C, 16-C

    Plasmid

    #124681

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-1 (PDCD1 Human)
    

    Expression

    Mammalian

    Promoter

    minimal CMV

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ7820 pHR (hU6-crPURI-EFS-PuroR-WPRE)

    Plasmid

    #214883

    Purpose

    Lentiviral vector encoding RfxCas13d targeting PURI guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crPURI-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLQ7854 pHR (hU6-crGLY-EFS-PuroR-WPRE)

    Plasmid

    #214884

    Purpose

    Lentiviral vector encoding RfxCas13d targeting GLY guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crGLY-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV_Nanog HMEJ donor

    Plasmid

    #97321

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Nanog. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Nanog HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV4Neo-murine IL-17RC(FL)

    Plasmid

    #46864

    Purpose

    expresses mouse IL-17RC (all exons)

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RC (Il17rc Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLL3.7m-psMEK2

    Plasmid

    #89363

    Purpose

    A lenti-viral vector expressing photoswitchable MEK2 in mammalian cells

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2017 Feb 24;355(6327):836-842.

    Insert

    psMEK2 (MAP2K2 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    Human influenza hemagglutinin (HA) tag and pdDron…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. Lenti_Efs_hSpCas9_NLS_FLAG-WPRE

    Plasmid

    #97313

    Purpose

    Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    humanized S. pyogenes Cas9
    

    Use

    Lentiviral and Mouse Targeting

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8f-p10

    Plasmid

    #218866

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8f
    

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. ACTN3-pEGFP

    Plasmid

    #52670

    Purpose

    Overexpression of GFP-tagged α-actinin-3

    Depositor

    Johannes Hell

    Article

    Hall et al Neuron. 2013 May 8;78(3):483-97. doi: 10

    Insert

    ACTN3 (ACTN3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. pBabe_puro_DEST_Flag_PHC3

    Plasmid

    #45262

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    PHC3 (PHC3 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 23, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pUC19_Ntag_BbsI-Mammalian

    Plasmid

    #186674

    Purpose

    N-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    Mammalian N-terminal Tag
    

    Tags

    3xFlag-3xHA

    Expression

    Mammalian

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pME-mScarlet3-Giantin (JDW 1356)

    Plasmid

    #224502

    Purpose

    Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

    Depositor

    Joshua Wythe

    Article

    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

    Insert

    mScarlet3-Giantin
    

    Use

    Gateway cloning

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. single polypeptide FPX biosensor for caspase-8

    Plasmid

    #60884

    Purpose

    FPX biosensor for caspase-8

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for caspase-8
    

    Tags

    HindIII site and NES sequence LALKLAGLDIGS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pSS1 (hLPL-FLAG)

    Plasmid

    #102447

    Purpose

    Expresses FLAG-tagged human LPL in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

    Insert

    Lipoprotein lipase (LPL Human)
    

    Tags

    6x His and FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pSP64-hKCNQ1

    Plasmid

    #53048

    Purpose

    Expresses alpha subunit for hKCNQ1 channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

    Insert

    Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)
    

    Use

    Xenopus oocyte expression

    Promoter

    SP6

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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30. HiBiT-FZD10

    Plasmid

    #195848

    Purpose

    Expresses human FZD10 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD10 (FZD10 Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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