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pTE104

Plasmid

#59399

Purpose

MethyloBrick expression vector, coxB promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PcoxB

Available Since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-GFP-Ubl4A

Plasmid

#86921

Purpose

To express Ubl4A tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDendra2-H2a

Plasmid

#157816

Purpose

Fluorescent reporter for histone H2A tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

H2A (H2ac13 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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OPN(-213)-luc

Plasmid

#31107

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

osteopontin promoter (SPP1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -213 to +87

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pRc/CMV HA TrkB-T1

Plasmid

#39980

Depositor

Yves-Alain Barde

Article

Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

Insert

TrkB.T1 (Ntrk2 Rat)

Tags

BM40 signal sequence and HA

Expression

Mammalian

Mutation

TrkB.T1 variant; endogenous signal sequence was r…

Promoter

CMV

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only
pJBJZ_001

Plasmid

#155357

Purpose

Gentamycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

Depositor

Elisabeth Raleigh

Article

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJBJZ_006

Plasmid

#155359

Purpose

Zeomycin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

Depositor

Elisabeth Raleigh

Article

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-tag2B-PISP/AIPP1

Plasmid

#47606

Depositor

Emanuel Strehler

Article

Goellner et al Ann N Y Acad Sci. 2003 Apr;986:461-71.

Insert

PISP (PDZD11 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
TOPO pTRE

Plasmid

#68457

Purpose

TOPO construct expressing pTRE for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Tetracycline Response Element Promoter

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pGSTag Bmf

Plasmid

#24264

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bmf (BMF Human)

Tags

Gst

Expression

Bacterial

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pRS406 ATG9 stopGFP

Plasmid

#114669

Purpose

Expresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tag

Depositor

Daniel Klionsky

Article

Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

Insert

ATG9

Tags

GFP

Expression

Yeast

Mutation

Changed the three linker base pairs after the las…

Promoter

ATG9

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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BcLOV-mCh-EGFR

Plasmid

#208274

Purpose

Expresses BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

BcLOV-mCherry-EGFR

Use

Lentiviral

Tags

mCherry

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRU323

Plasmid

#167690

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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RP2-EGFP

Plasmid

#86073

Purpose

A mammalian expression plasmid encoding retinitis pigmentosa 2 (RP2) with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

RP2 (RP2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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TAL2431

Plasmid

#36830

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HDAC2-TALEN_Right (Hdac2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pMK455 (dTAG-mClover-BSD)

Plasmid

#214385

Purpose

dTAG-mClover for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

dTAG-mClover-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMK456 (dTAG-mCherry-BSD)

Plasmid

#214386

Purpose

dTAG-mCherry for C-terminal tagging

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Insert

dTAG-mCherry-BSD

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-eGtACR1-mRuby2-ST

Plasmid

#109136

Purpose

Anion channelrhodopsin GtACR1 fused to ER export signal, mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eGtACR1-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRU292

Plasmid

#167687

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLPR2

Plasmid

#209980

Purpose

Contains Level 0 Part: resistance cassette (cat) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

resistance cassette (cat)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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