159,557 results

1. pCMVHA hEZH2

   Plasmid

   #24230

   Purpose

   Mammalian expression of human EZH2 with HA tag

   Depositor

   Kristian Helin

   Article

   Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE EF1α dCas9-VP64

   Plasmid

   #50918

   Purpose

   Constitutive dCas9-VP64 lentiviral expression vector

   Depositor

   Scot Wolfe, Rene Maehr

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   EF1alpha

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. hC73S-pEGFP-N1

   Plasmid

   #111536

   Purpose

   Expresses human KCNB1 C73S in mammalian cells for imaging

   Depositor

   Federico Sesti

   Insert

   human C73S-Kv2.1 (KCNB1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C73S

   Promoter

   CMV

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTriEx4-Cdc42-2G

   Plasmid

   #68814

   Purpose

   FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

   Depositor

   Olivier Pertz

   Article

   Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

   Insert

   Cdc42 activation reporter
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYX-C-mScarlet-Zeo-TSC22D2

   Plasmid

   #222670

   Purpose

   endogenously tag TSC22D2 with C-terminal mScarlet

   Depositor

   Jason Moffat

   Article

   Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

   Insert

   TSC22D2 (TSC22D2 Human)
   

   Use

   Tags

   mScarlet

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDisplay-GRAB_NPY1.0

   Plasmid

   #208675

   Purpose

   Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSynSRE-T-Luc

   Plasmid

   #60444

   Purpose

   Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.

   Depositor

   Timothy Osborne

   Article

   Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.

   Insert

   HMG-CoA synthase promoter (-324/-225)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   HMG-CoA synthase (-324/-225) & TATAA (-28/+39)

   Available since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO {hCAR}off-{GCaMP6f}on-W3SL

   Plasmid

   #111394

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection), Cre-ON GCaMP6f (ultrasensitive calcium sensor), and W3SL regulatory cassette (for maximize cloning capacity)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   hCAR (CXADR Human)
   GCaMP6f
   

   Use

   AAV

   Tags

   6xHis, Myc, T7, and Xpress

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-dDIO hChR2(H134R)-EYFP

   Plasmid

   #55640

   Purpose

   Dre-Dependent ChR2-EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   hChR2(H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Aug. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_PER1_WT

    Plasmid

    #82152

    Purpose

    Gateway Donor vector containing PER1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PER1 (PER1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. FABP1A

    Plasmid

    #42344

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FABP1A (FABP1 Human)
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pLR5-CAG-NLS-SNAP

    Plasmid

    #174879

    Purpose

    PiggyBac transposon vector for expression of NLS-SNAP

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    NLS-SNAP
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP

    Plasmid

    #90008

    Purpose

    Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    NBirA*-linker-FKBP
    FRB-linker-CBirA*
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV CEPIA3mt

    Plasmid

    #58219

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA3mt
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V E334D M339L

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMD.RVG.CVS24-N2c

    Plasmid

    #19712

    Depositor

    Manfred Schubert

    Article

    Mentis et al J Neurosci Methods. 2006 Oct 30. 157(2):

    Insert

    Rabies virus CVS24-N2c glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

    Plasmid

    #209079

    Purpose

    Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    sfGFP
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmax pona 12xTRICK 24xMS2SL

    Plasmid

    #64542

    Purpose

    TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    12x PP7 stem-loops
    

    Use

    Tags

    24xMS2 stem loops, HA, NLS, and dNP799

    Expression

    Mammalian

    Mutation

    Promoter

    ponA

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 Tat HA (P#29)

    Plasmid

    #14654

    Depositor

    Matija Peterlin

    Article

    Cujec et al Genes Dev. 1997 Oct 15. 11(20):2645-57.

    Insert

    Tat (tat HIV)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-Raichu-RhoA-CR

    Plasmid

    #40258

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Raichu-RhoA-CR (RHOA Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGFPmAID

    Plasmid

    #174213

    Purpose

    GFP::mAID donor backbone

    Depositor

    Masato Kanemaki

    Article

    Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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