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We narrowed to 1,212 results for: Ncl

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Showing: 1111 - 1140 of 1212 results

  6. pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T

    Plasmid
    #191331
    Purpose
    Expresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
    Expression
    Mammalian
    Mutation
    DYNC1H1 motor domain aa 1453-4644
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191331-plasmid-map-sequence-id-381751

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  9. pET24TEV-tSHIP2

    Plasmid
    #183771
    Purpose
    Expresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backbone
    Depositor
    William Kerr
    Article
    Sudan et al Front Immunol. 2022 May 4;13:830961. doi
    Insert
    SHIP2 (INPPL1 Human)
    Tags
    6HIS and Tobacco Etch Virus (TEV) Cleavage site
    Expression
    Bacterial
    Mutation
    includes aa18 to aa985 only
    Promoter
    T7 lac
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183771-plasmid-map-sequence-id-362691

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  13. pBABE-MCS(extended)-Puro (empty vector)

    Plasmid
    #172603
    Purpose
    Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Type
    Empty backbone
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    The multiple cloning site (MCS) has been edited/e…
    Available Since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172603-plasmid-map-sequence-id-340216

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  15. pB–DualPE

    Plasmid
    #235161
    Purpose
    For plant prime editing including large DNA fragment editing in wheat plants or other monocotyledons
    Depositor
    Zong Yuan
    Article
    Zhao et al Nat Plants. 2025 Feb;11(2):191-205. doi:
    Inserts
    Csy4-P2A-Nls-nCas9-NC-nls-MLV-Nls
    CmYLCV-epegRNA-CaMV poly(A) signal
    Use
    CRISPR
    Mutation
    Detailed in manuscript
    Available Since
    Oct. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    235161-plasmid-map-sequence-id-488642

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  21. G9A[SET]-dCas9

    Plasmid
    #100089
    Purpose
    Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Article
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    G9A (EHMT2 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-G9A[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    G9A[SET] includes aa 829-1209
    Promoter
    CMV
    Available Since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100089-plasmid-map-sequence-id-197561

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  24. pMDC32B-AtTAS1c-D2-B/c-AtMIR173

    Plasmid
    #137885
    Purpose
    Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.
    Depositor
    Alberto Carbonell
    Article
    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583
    Inserts
    AtTAS1c-D2-B/c
    2x35S-AtMIR173-Tnos
    Expression
    Plant
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Available Since
    May 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    137885-plasmid-map-sequence-id-275856

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  26. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

    Plasmid
    #194190
    Purpose
    Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)
    Depositor
    Ralf Brandes, Matthias Leisegang
    Article
    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533
    Insert
    SMANTIS promoter (1kb) (SMANTIS Human)
    Use
    Luciferase
    Mutation
    5 mutated KLF binding sites (positions 449,548,73…
    Promoter
    SMANTIS promoter (1kb), mutated
    Available Since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    194190-plasmid-map-sequence-id-381947

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  29. pcDNA3-eGFP-NLS-RNF168 delta RING

    Plasmid
    #133983
    Purpose
    mammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)
    Depositor
    Daniel Durocher
    Article
    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.
    Insert
    RNF168 (RNF168 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    deletion of RING; siRNA resistant sequence: GAGGA…
    Promoter
    CMV
    Available Since
    Nov. 4, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    133983-plasmid-map-sequence-id-261913

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  30. pHR-mCherry-p2A-LIC1 C-terminal half

    Plasmid
    #74603
    Purpose
    expresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherry
    Depositor
    Ron Vale
    Article
    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554
    Insert
    full length human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
    Use
    Lentiviral
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    amino acids 389-523 of LIC1
    Promoter
    SFFV
    Available Since
    May 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    74603-plasmid-map-sequence-id-141268

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Showing: 1111 - 1140 of 1212 results