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CMV-huCof R21Q-mRFP

Plasmid

#245964

Purpose

Expression of the weak F-actin binding R21Q cofilin-mRFP which serves as a cofilactin rod reporter without inducing rods

Depositor

James Bamburg

Article

Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

R21Q

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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huCof K22Q-mRFP

Plasmid

#245965

Purpose

Expression of the weak F-actin binding K22Q cofilin-mRFP which serves as a cofilactin rod reporter without inducing rods

Depositor

James Bamburg

Article

Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

K22Q

Promoter

CMV

Available Since

Oct. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pTRE3G-NlucP

Plasmid

#162595

Purpose

C-terminally PEST-tagged NanoLuc under TRE3G promoter

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

NanoLuc-PEST

Tags

PEST

Expression

Mammalian

Promoter

TRE3G promoter

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRsetB-his7-Perceval

Plasmid

#20336

Depositor

Gary Yellen

Article

Berg et al Nat Methods. 2009 Feb;6(2):161-6. doi: 1

Insert

Perceval (fluorescent ATP:ADP sensor)

Tags

his(7)

Expression

Bacterial

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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pActP-GCaMP6m

Plasmid

#64714

Purpose

visualizing calcium in cultured Drosophila S2 cells

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

GCaMP6m

Expression

Insect

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pFGL822

Plasmid

#58225

Purpose

For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.

Depositor

Naweed Naqvi

Article

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

phosphinothricin acetyl transferase

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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MyrPalm-CFP

Plasmid

#14867

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

CFP

Tags

Myr/Palm

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pFGL821-8

Plasmid

#58224

Purpose

For fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.

Depositor

Naweed Naqvi

Article

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

hygromycin B phosphotransferase

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pRL1063a

Plasmid

#70687

Purpose

pRL1058 with Vibrio fischeri luxAB for sensing

Depositor

Peter Wolk

Article

Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

Insert

Transposon Tn5-1063

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pFGL820

Plasmid

#58221

Purpose

For fungal transformation via ATMT. Transformants to be selected by sulfonylurea (specially chlorimuro ethyl) resistance.

Depositor

Naweed Naqvi

Article

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

ILV2

Expression

Bacterial

Promoter

ILV2 native promoter

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pC4-RHE-PAS

Plasmid

#39869

Depositor

Vladislav Verkhusha

Article

Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

Insert

iRFP protein PAS domain

Tags

FRB

Promoter

CMV

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pUASTattB-p65AD::CaM

Plasmid

#64717

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

p65AD::CaM

Expression

Insect

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pQUAST-MKII::GAL4DBDo

Plasmid

#64723

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

MKII::GAL4DBDo

Expression

Insect

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pQUAST-p65AD::CaM

Plasmid

#64715

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

p65AD::CaM

Expression

Insect

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pJ2042.2_invRep

Plasmid

#140688

Purpose

3-color stripe (inverted reporters)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

3-color stripe (inverted reporters)

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pUASTattB-MKII::nlsLexADBDo

Plasmid

#64718

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

MKII::nlsLexADBDo

Expression

Insect

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only

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pFGL820-2

Plasmid

#58222

Purpose

For fungal transformation via ATMT. Transformants to be selected by sulfonylurea (specially chlorimuro ethyl) resistance.

Depositor

Naweed Naqvi

Article

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

ILV2

Expression

Bacterial

Promoter

ILV2 native promoter

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pROSA26-SAE

Plasmid

#208338

Purpose

ROSA26 targeting vector for the SAE sensor for ERK1/2 signaling

Depositor

Philippe Soriano

Article

Dinsmore et al Dev Biol. 2023 Sep 1:S0012-1606(23)00146

Insert

SAE sensor

Use

Mouse Targeting

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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GLuc VLP

Plasmid

#182213

Purpose

YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Gaussia luciferase tagged with VP2C (GAL10 promoter).

Depositor

Claudia Vickers

Inserts

VP2C-GLuc
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pQUAST-M13::GAL4DBDo

Plasmid

#64724

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

M13::GAL4DBDo

Expression

Insect

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-HaloTag7-LC3

Plasmid

#184899

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-LoxP-mKate-LoxP-EGFP

Plasmid

#167940

Purpose

CMV promoter-LoxP-mKate-LoxP-EGFP

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

LoxP-mKate-LoxP-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IB-HaloTag7-mGFP

Plasmid

#184903

Purpose

Stable expression of HaloTag7-mGFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

monomeric EGFP

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAG148 FRB-NFAST

Plasmid

#130812

Purpose

Expresses FRB-NFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

FRB-NFAST

Tags

myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAG153 FKBP-CFAST11

Plasmid

#130813

Purpose

Expresses FKBP-CFAST11 in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

FKBP-CFAST11

Tags

Myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-Tol2 transposase

Plasmid

#158774

Purpose

Expression of Tol2 transposase

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

Tol2 tranposase

Expression

Mammalian

Mutation

Tol2 codon usage frequency well suitable for muri…

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMRX-No-HaloTag7-mGFP-LC3

Plasmid

#184901

Purpose

Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAG241 FKBP-CFAST10

Plasmid

#130814

Purpose

Expresses FKBP-CFAST10 in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

FKBP-CFAST10

Tags

Myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Rosa26 mT/mG

Plasmid

#17787

Depositor

Liqun Luo

Article

Muzumdar et al Genesis. 2007 Sep . 45(9):593-605.

Insert

Rosa26 mT/mG

Use

Cre/Lox and Mouse Targeting

Tags

membrane tag

Expression

Mammalian

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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pMPRAdonor2

Plasmid

#49353

Purpose

minP-luc2 donor plasmid for MPRA library construction

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

luc2

Use

Luciferase

Promoter

minP

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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