We narrowed to 11,881 results for: pcDNA
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Plasmid#113944Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutininDepositorInsertT1R2 (TAS1R2 Human)
UseTagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-839PromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Neo(+) hSUMF1
Plasmid#24386DepositorInserthSUMF1 (SUMF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GEM2-Halo-FRB
Plasmid#197056PurposeExpression of Synechococcus elongatus encapsulin Halo-FRB-fusion in mammalian cellsDepositorInsertEncapsulin
UseTagsFRB and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL4A-FLAG
Plasmid#15821DepositorInsertCullin4A (CUL4A Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N440PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαs-LgB113
Plasmid#134362PurposeNanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.DepositorInsertGαs-LgB113 (GNAS Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
2MLS-HyperNova/pcDNA3
Plasmid#221457PurposeExpressing HyperNova in mitochondriaDepositorInsertHyperNova fused with a tandem repeat of the mitochondria localization signal from cytochrome c oxidase subunit VIII
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL4B-FLAG
Plasmid#15822DepositorInsertCullin4B (CUL4B Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N594PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL5-FLAG
Plasmid#15823DepositorInsertCullin5 (CUL5 Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N441PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL2-FLAG
Plasmid#15819DepositorInsertCullin2 (CUL2 Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N427PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 SIRT1-FL
Plasmid#105670PurposeMammalian expression construct of mouse SIRT1 full length isoformDepositorInsertSIRT1 (Sirt1 Mouse)
UseTagsExpressionMammalianMutationSynonymous base changes not affecting the protein…PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAR4-NLS
Plasmid#138217PurposeNuclear-targeted FRET-based PKA activity reporter.DepositorInsertAKAR4-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαq-LgB97
Plasmid#134360PurposeNanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.DepositorInsertGαq-LgB97 (GNAQ Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi3-LgB91
Plasmid#134359PurposeNanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.DepositorInsertGαi3-LgB91 (GNAI3 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi2-LgB91
Plasmid#134358PurposeNanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.DepositorInsertGαi2-LgB91 (GNAI2 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα12-LgB115
Plasmid#134363PurposeNanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.DepositorInsertGα12-LgB115 (GNA12 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAR4-Kras
Plasmid#61621PurposeNon-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activityDepositorInsertAKAR4-Kras
UseTagsC-terminal targeting sequence from Kras, Cerulean…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAR4-NES
Plasmid#64727PurposeCytosol-targeted, FRET-based PKA activity reporter.DepositorInsertAKAR4-NES
UseTagsCerulean, NES, and cpVenus[E172]ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα11-LgB97
Plasmid#134361PurposeNanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.DepositorInsertGα11-LgB97 (GNA11 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα13-LgB106
Plasmid#134364PurposeNanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.DepositorInsertGα13-LgB106 (GNA13 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-AKAR4
Plasmid#61620PurposeLipid raft-targeted FRET biosensor for monitoring PKA activityDepositorInsertLyn-AKAR4
UseTagsCerulean, N-terminal targeting sequence from Lyn …ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAP79-AKAR4
Plasmid#181957PurposeGenetically encoded FRET-based PKA activity reporter fused to AKAP79.DepositorInsertAKAP79-AKAR4 (AKAP5 Human)
UseTagsAKAP79, Cerulean, HIS tag, and cpVenus(E172)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-BIRC3-SU
Plasmid#160699PurposeExpresses GFP-BIRC3 with the short 3'UTR in mammalian cellsDepositorInsertGFP-BIRC3 (BIRC3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-BIRC3-LU
Plasmid#160700PurposeExpresses GFP-BIRC3 with the long 3'UTR in mammalian cellsDepositorInsertGFP-BIRC3 (BIRC3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-mEGFP
Plasmid#191847PurposeExpresses the monomeric form of EGFPDepositorInsertmEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo-mEGFP
Plasmid#214145PurposeExpresses the monomeric EGFP in eukaryotic cellsDepositorInsertmEGFP
UseTagsExpressionMammalianMutationA207K L221K F223RPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Bsd-mEGFP
Plasmid#214369PurposeExpression of the monomeric Green Fluorescent ProteinDepositorInsertmEGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lifeact_HaloTag9
Plasmid#175545PurposeExpression of HaloTag9 at the actin filaments of mammalian cellsDepositorInsertLifeact-HaloTag9
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
ELP-TEMP/pcDNA3.1(-)
Plasmid#178434PurposeGenetically encoded ratiometric fluorescent thermometerDepositorInsertELP-TEMP
UseTagsNo tagExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-REL
Plasmid#27253DepositorInsertREL (REL Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_H2B_HaloTag7
Plasmid#169329PurposeExpression of H2B-HaloTag7 in mammalian cellsDepositorInsertH2B-HaloTag7 (H2BC21 Human)
UseTagsHaloTag7ExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Myc-IQGAP1
Plasmid#30118DepositorInsertIQGAP1 (IQGAP1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hPTGS2-2flag
Plasmid#102498PurposeMammalian expression of PTGS2DepositorInsertPTGS2 (PTGS2 Human)
UseTagsFlag-FlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PTK7-VSV
Plasmid#65250PurposeExpression of VSV tagged PTK7 in mammalian cellsDepositorInsertPTK7 (PTK7 Human)
UseTagsVSVExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PTK7DN-VSV
Plasmid#65251PurposeExpression of VSV tagged dominant negative PTK7 in mammalian cellsDepositorInsertPTK7DN (PTK7 Human)
UseTagsVSVExpressionMammalianMutationdeleted AA 737-1071PromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMax-Synectin
Plasmid#35943DepositorInsertSynectin (Gipc1 Mouse)
UseTags6xHIS and XpressExpressionMammalianMutationK111RPromoterCMVAvailable sinceJune 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/mStayGold(c4)=UtrCH
Plasmid#212020PurposeFor filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).DepositorInsertUtrCH
UseTagsmStayGold(c4)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb
Plasmid#175544PurposeExpression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag9-myc_PDGFRtmb
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.3 N-MYC TIP60
Plasmid#213003Purposeexpresses human TIP60DepositorInsertKAT5 (KAT5 Human)
UseTagsMYCExpressionMammalianMutationPromotercmvAvailable sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
p52 cFlag pcDNA3
Plasmid#20019DepositorInsertNfkb2 (Nfkb2 Mouse)
UseTagsFlagExpressionMammalianMutationTruncated at the p100 ligation site. It codes for…PromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP
Plasmid#169332PurposeNuclear expression of HaloTag9 in mammalian cellsDepositorInsertH2B-HaloTag9 (H2BC21 Human)
UseTagsHaloTagExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only