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Showing: 1851 - 1900 of 9150 results

  2. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41732-plasmid-map-sequence-id-210671

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  6. P48

    Plasmid
    #212155
    Purpose
    Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.
    Depositor
    Kang Zhou
    Insert
    superfolder green fluorescent protein, secondary alcohol dehydrogenase
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212155-plasmid-map-sequence-id-420758

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  12. pJMP12049

    Plasmid
    #222355
    Purpose
    Tn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.
    Depositor
    Jason Peters
    Insert
    sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PabstBR
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222355-plasmid-map-sequence-id-439062

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  16. pET MBP mRuby2 LIC cloning vector (MBP-mRuby2)

    Plasmid
    #86933
    Purpose
    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mRuby2
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86933-plasmid-map-sequence-id-174748

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  23. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196721-plasmid-map-sequence-id-389753

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  24. pKP2299

    Plasmid
    #167627
    Purpose
    Express TonB C18G, H20A, A209C.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    tonB C18G, H20A, A209C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tonB with C18G, H20A, A209C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167627-plasmid-map-sequence-id-339947

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  25. pKP1861

    Plasmid
    #167629
    Purpose
    Express TonB C18G, H20A, R212C.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    tonB C18G, H20A, R212C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tonB with C18G, H20A, R212C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167629-plasmid-map-sequence-id-339949

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  26. pKP1723

    Plasmid
    #167631
    Purpose
    Express TonB C18G, H20A, R214C.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    tonB C18G, H20A, R214C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tonB with C18G, H20A, R214C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167631-plasmid-map-sequence-id-339950

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  27. pKP2303

    Plasmid
    #167632
    Purpose
    Express TonB C18G, Q160C, F202A.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    tonB C18G, Q160C, F202A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tonB with C18G, Q160C, F202A mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167632-plasmid-map-sequence-id-339486

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  28. pKP1692

    Plasmid
    #167625
    Purpose
    Express TonB C18G, H20A, N208C.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    tonB C18G, H20A, N208C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tonB with C18G, H20A, N208C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167625-plasmid-map-sequence-id-337552

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  29. pKP1722

    Plasmid
    #167623
    Purpose
    Express TonB C18G, H20A, V206C.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    tonB C18G, H20A, V206C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tonB with C18G, H20A, V206C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167623-plasmid-map-sequence-id-337550

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  30. pKP1868

    Plasmid
    #167621
    Purpose
    Express TonB C18G, H20A, R204C.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    tonB C18G, H20A, R204C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tonB with C18G, H20A, R204C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167621-plasmid-map-sequence-id-337549

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  31. pKP1720

    Plasmid
    #167619
    Purpose
    Express TonB C18G, H20A, M201C.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    tonB C18G, H20A, M201C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tonB with C18G, H20A, M201C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167619-plasmid-map-sequence-id-337547

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  32. pKP1708

    Plasmid
    #167617
    Purpose
    Express TonB C18G, H20A, Q160C.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    tonB C18G, H20A, Q160C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    tonB with C18G, H20A, Q160C mutations.
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167617-plasmid-map-sequence-id-337543

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  33. pLVX-TetOne-Puro-EcSTH-FLAG

    Plasmid
    #218628
    Purpose
    This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.
    Depositor
    Valentin Cracan
    Article
    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038
    Insert
    EcSTH
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218628-plasmid-map-sequence-id-432188

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  34. pLVX-TetOne-Puro-mitoEcSTH-FLAG

    Plasmid
    #218629
    Purpose
    This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.
    Depositor
    Valentin Cracan
    Article
    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038
    Insert
    mitoEcSTH
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG and MTS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218629-plasmid-map-sequence-id-432199

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  36. pSP6-RNAP

    Plasmid
    #48143
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2
    Insert
    rna polymerase SP6
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    V314A (numbering based on NCBI Sequence ID: NP_85…
    Promoter
    Available since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
    48143-plasmid-map-sequence-id-373044

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  37. pGRG36-Kn-PA1-GFP

    Plasmid
    #79088
    Purpose
    pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)
    Depositor
    Frank Rosenzweig
    Article
    Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)
    Insert
    GFP
    Use
    Bacterial chromosome integration and expression o…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PA1
    Available since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    79088-plasmid-map-sequence-id-182383

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  39. pDEINO1

    Plasmid
    #179472
    Purpose
    Multi-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugation
    Depositor
    Bogumil Karas
    Article
    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1
    Inserts
    ori
    cat
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    179472-plasmid-map-sequence-id-375147

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  40. pJL1-MBP-4xDQNAT

    Plasmid
    #128390
    Purpose
    In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Maltose binding protein
    Use
    Tags
    4xDQNAT glycosylation tag and 6xHis tag
    Expression
    Bacterial
    Mutation
    A302T- please see depositor comment
    Promoter
    T7
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128390-plasmid-map-sequence-id-260183

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  42. pCML 961

    Plasmid
    #98848
    Purpose
    pTriFC_aidB. Expresses protein-NYFP and 2MS2BD-5' UTR mStrawberry fusions in E. coli for Dual-fluorescence TriFC assay (ampR).
    Depositor
    Lydia Contreras
    Article
    Sowa et al Nucleic Acids Res. 2017 Feb 28;45(4):167
    Inserts
    5' UTR + first 100 nucleotides of coding sequence of the aidB gene
    csrA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pLacO
    Available since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98848-plasmid-map-sequence-id-194122

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  43. PatoM-LOG241

    Plasmid
    #72847
    Purpose
    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. LOG241 backbone.
    Depositor
    Chris Barnes
    Article
    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278
    Insert
    PatoM
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PatoM
    Available since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    72847-plasmid-map-sequence-id-133613

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  44. pCS2+ oDam_f_GFPoNLS

    Plasmid
    #85819
    Purpose
    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    oDam_f_GFPoNLS
    Use
    Tags
    oNLS (optimized nuclear localization signal) C te…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85819-plasmid-map-sequence-id-170478

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  45. FB005

    Plasmid
    #119678
    Purpose
    Coding sequence nptII from E. coli domesticated into pUPD2 with AATG/GCTT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    geneticin resistance marker
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119678-plasmid-map-sequence-id-237143

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Showing: 1851 - 1900 of 9150 results