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We narrowed to 2,087 results for: Endog

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Showing: 1851 - 1900 of 2087 results

  6. ppk301-T2A-QF2 HDR plasmid

    Plasmid
    #130667
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene
    Depositor
    Leslie Vosshall
    Article
    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1
    Insert
    ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm
    Use
    Donor template
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    130667-plasmid-map-sequence-id-257080

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  8. pSPObooster

    Plasmid
    #216160
    Purpose
    Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains
    Depositor
    Grant Brown
    Article
    Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978
    Inserts
    MKT1(30G) (MKT1 Budding Yeast)
    RME1(ins-308A) (RME1 Budding Yeast)
    Expression
    Yeast
    Mutation
    MKT1 gene containing an aspartic acid to glycine …
    Promoter
    endogenous promoter
    Available Since
    May 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    216160-plasmid-map-sequence-id-433867

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  14. Th-iCre

    Plasmid
    #159459
    Purpose
    Targeting vector to TH gene locus for induction of P2A-fused iCre, with PGK-puroR drug resistant cassette flanked by FRT sequence.
    Depositor
    Yuejun Chen
    Article
    Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-
    Insert
    P2A-iCre
    Use
    Cre/Lox
    Tags
    P2A
    Expression
    Mammalian
    Promoter
    Endogenous TH
    Available Since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    159459-plasmid-map-sequence-id-319220

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  15. IR25a-T2A-QF-AD

    Plasmid
    #162526
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Ir25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm
    Expression
    Insect
    Available Since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    162526-plasmid-map-sequence-id-354666

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  16. ORCO-T2A-QF-DBD

    Plasmid
    #162525
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses the DNA-binding domain of QF by knocking-in a T2A-QF-DBD fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Orco-left-HDR-arm; T2A-Zip-::QFDBD-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
    Expression
    Insect
    Available Since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    162525-plasmid-map-sequence-id-354642

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  20. pBrain-GFP-TACC3KDP-shTACC3

    Plasmid
    #59356
    Purpose
    Knocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.
    Depositor
    Stephen Royle
    Article
    Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10
    Insert
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    Use
    RNAi
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Silent mutations in amino acids 30-33 to confer s…
    Promoter
    CMV
    Available Since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    59356-plasmid-map-sequence-id-94885

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  28. pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

    Plasmid
    #140228
    Purpose
    RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.
    Depositor
    Stanley Qi
    Article
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Insert
    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    Use
    Lentiviral
    Tags
    BFP
    Expression
    Mammalian
    Promoter
    RRM2 endogenous promoter
    Available Since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140228-plasmid-map-sequence-id-291990

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  29. pONSY-CoNMM:mCherry

    Plasmid
    #111878
    Purpose
    This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
    Use
    Capsaspora owczarzaki
    Tags
    mCherry
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111878-plasmid-map-sequence-id-213490

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  30. pONSY-CoH2B:Venus

    Plasmid
    #111877
    Purpose
    This plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Capsaspora Histone H2B (CoH2B) fused to Venus
    Use
    Capsaspora owczarzaki
    Tags
    Venus
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111877-plasmid-map-sequence-id-213489

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  31. ORCO-T2A-QF2

    Plasmid
    #162524
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Orco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
    Expression
    Insect
    Available Since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    162524-plasmid-map-sequence-id-354668

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  33. Gr4-T2A-QF2 HDR plasmid

    Plasmid
    #140944
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Gr4 gene
    Depositor
    Leslie Vosshall
    Article
    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12
    Insert
    Gr4-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr4-right-HDR-arm
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140944-plasmid-map-sequence-id-313602

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  34. Sdk1-AP-His

    Plasmid
    #72008
    Purpose
    Expresses the extracellular region of the Sdk1 protein (endogenous signal peptide replaced with CD33 signal peptide), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Sdk1 (Sdk1 Mouse)
    Tags
    AP-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72008-plasmid-map-sequence-id-135630

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  35. Sdk1-Fc-His

    Plasmid
    #72134
    Purpose
    Expresses the extracellular region of the Sdk1 protein (endogenous signal peptide replaced with CD33 signal peptide), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Sdk1 (Sdk1 Mouse)
    Tags
    Fc-His
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72134-plasmid-map-sequence-id-139915

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  38. pJL358

    Plasmid
    #243712
    Purpose
    A piggyBac vector containing the COX7A2 coding sequence (CDS) flanked by the 5' UTR of the COX7A2 gene and 3' UTR of the ACTB gene
    Depositor
    Jonathan Weissman
    Article
    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.
    Insert
    5' UTR and CDS of COX7A2, followed by the 3' UTR of the ACTB gene (COX7A2 Human)
    Expression
    Mammalian
    Promoter
    endogenous promoter
    Available Since
    Nov. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    243712-plasmid-map-sequence-id-489307

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  43. IR76b-T2A-QF2

    Plasmid
    #162523
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir76b gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Ir76b-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir76b-right-HDR-arm
    Expression
    Insect
    Available Since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    162523-plasmid-map-sequence-id-354669

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  44. GR3-T2A-QF2

    Plasmid
    #162521
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Gr3 gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Gr3-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr3-right-HDR-arm
    Expression
    Insect
    Available Since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    162521-plasmid-map-sequence-id-372762

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  45. IR8a-T2A-QF2

    Plasmid
    #162520
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir8a gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Ir8a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir8a-right-HDR-arm
    Expression
    Insect
    Available Since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    162520-plasmid-map-sequence-id-372758

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  46. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid
    #184774
    Purpose
    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.
    Depositor
    Zhiping Xie
    Article
    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10
    Insert
    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Mouse, Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    184774-plasmid-map-sequence-id-382498

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  49. IR25a-T2A-QF2

    Plasmid
    #162522
    Purpose
    Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene
    Depositor
    Leslie Vosshall
    Article
    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.
    Insert
    Ir25a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir25a-right-HDR-arm
    Expression
    Insect
    Available Since
    Aug. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    162522-plasmid-map-sequence-id-354640

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  50. pORANGE_NFL linker-3xFLAG KI

    Plasmid
    #182679
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
    Expression
    Mammalian
    Promoter
    U6 and chicken beta-actin promoter
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182679-plasmid-map-sequence-id-363017

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Showing: 1851 - 1900 of 2087 results