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pHKMC1: Empty sgRNA for Cloning

Plasmid

#67720

Purpose

Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.

Depositor

Monica Colaiacovo

Article

Norris et al Genetics. 2015 Oct;201(2):449-58. doi: 1

Type

Empty backbone

Expression

Worm

Promoter

PU6

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pri-miR-16-1

Plasmid

#51382

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

MIR16-1 microRNA 16-1 (MIR16-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pET19b+H2B(T116C)

Plasmid

#157808

Purpose

Expression of human histone H2B with T116C mutation to facilitate maleimide chemistry

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H2B(T116C)

Expression

Bacterial

Mutation

T116C

Promoter

T7

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/myc-his Ndel1

Plasmid

#12572

Depositor

Li-Huei Tsai

Article

Shu et al Neuron. 2004 Oct 14. 44(2):263-77.

Insert

Ndel1 (NDEL1 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pJWV25

Plasmid

#61045

Purpose

To construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaA

Depositor

Jan-Willem Veening

Article

Eberhardt et al Mol Microbiol. 2009 Oct;74(2):395-408. d

Type

Empty backbone

Use

Synthetic Biology

Tags

GFP+

Expression

Bacterial

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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SLC30A10-GFP

Plasmid

#104381

Purpose

Expresses SLC30A10-GFP wild-type in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-326

Insert

SLC30A10 (SLC30A10 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSN007

Plasmid

#102440

Purpose

For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

Depositor

Alejandro Chavez, George Church

Article

Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

Insert

7SK paired gRNA insert

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Xenopus C-cadherin

Plasmid

#17023

Depositor

Barry Gumbiner

Article

Lee et al Dev Biol. 1995 Oct . 171(2):363-73.

Insert

C-cadherin (cdh3.S Frog)

Use

Xenopus expression

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
peCALWY-NB

Plasmid

#22239

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY non-binding variant

Expression

Mammalian

Mutation

C284S, C287S, C413S and C416S mutations linker c…

Available Since

Jan. 11, 2010

Availability

Academic Institutions and Nonprofits only

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pMSCV-Pidd1(T788A)

Plasmid

#60529

Purpose

Retroviral expression of Pidd1 T788A phosphorylation mutant

Depositor

Trudy Oliver

Article

Terry et al Cell Death Differ. 2014 Oct 10. doi: 10.

Insert

Pidd1 (PIDD1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Threonine 788 to Alanine*

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-pri-let-7g

Plasmid

#51381

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

pri-let-7g (MIRLET7G Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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UNC50-myc

Plasmid

#104382

Purpose

Expresses UNC50-myc wild-type in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Selyunin et al J Cell Biol. 2017 Oct 2;216(10):3249-326

Insert

UNC50 (UNC50 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pTK_Sox9_enh_349_Citrine

Plasmid

#130594

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox9_enh_349

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAE03

Plasmid

#61044

Purpose

To construct C-terminal GFP fusions, single crossover integration vector

Depositor

Jan-Willem Veening

Article

Eberhardt et al Mol Microbiol. 2009 Oct;74(2):395-408. d

Type

Empty backbone

Use

Synthetic Biology

Tags

GFP+

Expression

Bacterial

Available Since

Feb. 6, 2015

Availability

Academic Institutions and Nonprofits only

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RP2-EGFP

Plasmid

#86073

Purpose

A mammalian expression plasmid encoding retinitis pigmentosa 2 (RP2) with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

RP2 (RP2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-LANA

Plasmid

#157801

Purpose

Expression of mEGFP and LANA peptide fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-LANA

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMCSG7-D16A-NmeCas9

Plasmid

#71476

Purpose

bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-D16A-NmeCas9

Tags

6His

Expression

Bacterial

Mutation

D16A

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pMCSG7-H588A-NmeCas9

Plasmid

#71477

Purpose

bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-H588A-NmeCas9

Tags

6His

Expression

Bacterial

Mutation

H588A

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pri-let-7d

Plasmid

#51379

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

pri-let-7d (MIRLET7D Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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mCas13 Twin-SUMO

Plasmid

#199753

Purpose

Expresses codon optimized miniature mCas13 for protein expression in E.coli

Depositor

Magdy Mahfouz

Article

Mahas et al ACS Synth Biol. 2021 Oct 15;10(10):2541-

Insert

mCas13

Tags

SUMO

Expression

Bacterial

Promoter

LacI

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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