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SPLICS Po-ER Long P2A

Plasmid

#164113

Purpose

Detect the long Peroxisomes-Endoplasmic reticulum contact

Depositor

Tito Calì, Marisa Brini

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP Po-ER Long P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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SPLICS PM-ER Short P2A

Plasmid

#164112

Purpose

Detect the short Plasma membrane-Endoplasmic reticulum contact

Depositor

Tito Calì, Marisa Brini

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP PM-ER Short P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-syn-fRGECO2

Plasmid

#125244

Purpose

Expresses red fluorescent calcium sensor in hippocampal slices

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO2

Use

Tags

Expression

Mammalian

Mutation

W44Y, D432A

Promoter

synapsin

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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EYFP-hCaM

Plasmid

#47603

Depositor

Emanuel Strehler

Article

Rogers et al J Biol Chem. 2001 Apr 13;276(15):12182-9

Insert

CaM (CALM3 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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SPLICS Po-Mt Short P2A

Plasmid

#164116

Purpose

Detect the short Peroxisomes-mitochondria contact

Depositor

Tito Calì, Marisa Brini

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Po-Mt Short P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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SPLICS Po-Mt Long P2A

Plasmid

#164115

Purpose

Detect the long Peroxisomes-mitochondria contact

Depositor

Tito Calì, Marisa Brini

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Po-Mt Long P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ER-CaNAR2

Plasmid

#64732

Purpose

Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CYP450-CaNAR2

Use

Tags

Cerulean3, N-terminal 27 amino acids from CYP450,…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PM-CaNAR2

Plasmid

#64730

Purpose

Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

Lyn-CaNAR2

Use

Tags

Cerulean3, N-terminal targeting sequence from Lyn…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER- PM Short P2A

Plasmid

#164110

Purpose

Detect the short Plasma membrane-mitochondria-Endoplasmic reticulum contact

Depositor

Tito Calì, Marisa Brini

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP and YFP PM- Mt-ER Short P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

Plasmid

#203229

Purpose

Cre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Park et al bioRxiv. 2023 Jun 2:2023.06.02.543490. d

Insert

Cre-dependent, membrane-localized Optopatch

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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CapChR1_pmCherry-N1

Plasmid

#188031

Purpose

Expresses CapChR1-mCherry in mammalian cells

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR1-mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

S63D, L132C, T159C and N258E mutations in CrChR2

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-fRCaMP2

Plasmid

#125250

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRCaMP2

Use

Tags

Expression

Mammalian

Mutation

D396A

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-OptoPB

Plasmid

#113935

Purpose

Express a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactions

Depositor

Yubin Zhou

Article

He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

Insert

OptoPB

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER- Po Short P2A

Plasmid

#164118

Purpose

Detect the short Peroxisomes-mitochondria-Endoplasmic reticulum contact

Depositor

Tito Calì, Marisa Brini

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP and YFP Mt-ER- Po Short P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PP_070_pAAV_CAG_LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

Plasmid

#203228

Purpose

Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

Depositor

Adam Cohen

Article

Park et al bioRxiv. 2023 Jun 2:2023.06.02.543490. d

Insert

Membrane-localized optopatch for neuronal dendrites

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-GCaMP3bright

Plasmid

#74423

Purpose

Express fluorescent calcium indicator GCaMP3bright in mammalian cells

Depositor

Katalin Torok

Article

Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

Insert

GCaMP3bright

Use

Tags

Expression

Mammalian

Mutation

D392A

Promoter

CMV

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pKK233-hCaM

Plasmid

#47598

Depositor

Emanuel Strehler

Article

Rhyner et al Biochemistry. 1992 Dec 29;31(51):12826-3

Insert

CaM (CALM3 Human)

Use

Tags

Expression

Bacterial

Mutation

contains 469 bp fragment (nt 92-559)

Promoter

trc

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-GCaMP3fast

Plasmid

#74422

Purpose

Express fluorescent calcium indicator GCaMP3fast in mammalian cells

Depositor

Katalin Torok

Article

Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

Insert

GCaMP3fast

Use

Tags

Expression

Mammalian

Mutation

W4Y D356A

Promoter

CMV

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Mito-CaNAR2

Plasmid

#64731

Purpose

Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2

Use

Tags

Cerulean3, N-terminal 30 amino acids from DAKAP1,…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-syn-fRCaMP2

Plasmid

#125246

Purpose

Expresses red fluorescent calcium sensor in hippocampal slices

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRCaMP2

Use

Tags

Expression

Mammalian

Mutation

D396A

Promoter

synapsin

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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