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Showing: 741 - 760 of 13098 results

  5. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid
    #190598
    Purpose
    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter
    Depositor
    Claudia Rueckert
    Article
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    mCherry - separated by T2A
    Expression
    Mutation
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190598-plasmid-map-sequence-id-379953

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  10. Ccas9LacZ

    Plasmid
    #113083
    Purpose
    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.
    Depositor
    Tibor Vellai
    Article
    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    113083-plasmid-map-sequence-id-218959

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  12. e-ciCas9_pcDNA5

    Plasmid
    #100553
    Purpose
    Expresses enhanced specificity ciCas9 (e-ciCas9) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.
    Depositor
    Dustin Maly
    Article
    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n
    Insert
    e-ciCas9
    Use
    CRISPR; Frt, flp-in
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100553-plasmid-map-sequence-id-193863

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  13. pDONR221_zCas9

    Plasmid
    #213913
    Purpose
    Entry clone containing 3×FLAG-NLS-zCas9-NLS encoding sequence, Pisum sativum rbcS E9 terminator. For use in plants and compatible with the MultiSite Gateway system
    Depositor
    Sébastien Mongrand
    Article
    Li et al Bio Protoc. 2024 Jul 5;14(13):e5029. doi
    Insert
    zCas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    213913-plasmid-map-sequence-id-437263

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  17. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

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  20. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid
    #86612
    Purpose
    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86612-plasmid-map-sequence-id-168849

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Showing: 741 - 760 of 13098 results