We narrowed to 28,411 results for: REP

1. pMS33: pTwinStrep-SUMO-PmcTnsD

   Plasmid

   #168166

   Purpose

   PmcTnsD expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   PmcCAST TnsD
   

   Use

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET51b-Strep-hAGT-His

   Plasmid

   #167276

   Purpose

   Overexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   hAGT (AGT Human)
   

   Use

   Tags

   His x10 and Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXP420 v2 (repaired Amp promoter)

   Plasmid

   #86920

   Purpose

   Shuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.

   Depositor

   Gary Yellen

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-2xFLAG-2xSTREP-CCND3

   Plasmid

   #172623

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-REPORT(EF1a)-TTN

   Plasmid

   #172327

   Purpose

   LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETNKI-strepII-3C-LIC-kan

   Plasmid

   #108706

   Purpose

   Expression of a StrepII-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Use

   Tags

   StrepII-3C protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-2xFLAG-2xSTREP-CCND2

   Plasmid

   #172629

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-Strep-TEV-Nsp14

   Plasmid

   #154955

   Purpose

   Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli

   Depositor

   Justin Kollman

   Insert

   Strep-TEV-Nsp14 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Strep-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pUPD2:35S-TMV-Rep-MP

   Plasmid

   #106217

   Purpose

   Provides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp polymerase with intron, movement protein and sgCP promoter) as level 0 GoldenBraid part (GGAG-AATG)

   Depositor

   Tomáš Moravec

   Insert

   Infectious clone of Tobacco mosaic virus, 5' part
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET51b-Strep-SsOGT-H5-His

    Plasmid

    #167277

    Purpose

    Overexpression of a sulfolobal equivalent of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SsOGT-H5
    

    Use

    Tags

    His x10 and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJM1-zeo-2xStrep-BFLF1

    Plasmid

    #162661

    Purpose

    Lentiviral vector for stable expression of N-terminal 2xStrep-tagged BFLF1 in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    BFLF1
    

    Use

    Lentiviral

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.2 V5-DEST mir-1-1 reporter

    Plasmid

    #46646

    Purpose

    expression of query pri-miRNA

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir-1-1 reporter
    

    Use

    RNAi; Expression of query pri-mirna sequences in …

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-Cas9-VQR-GFP_activity_reporter

    Plasmid

    #87156

    Purpose

    This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

    Depositor

    Daniel Bauer

    Article

    Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

    Insert

    GFP and sgRNA targeting GFP (NGA restricted)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.2 mir-1-1 reporter mir16-1 wt

    Plasmid

    #46683

    Purpose

    Expresses wild-type miR-16-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir16-1 wt (MIR16-1 Synthetic, Human)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmiR-report-MYB-mut

    Plasmid

    #141115

    Purpose

    Reporter plasmid of mutant MYB in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    MYB (MYB Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    A to T mutations at m6A sites

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1kb Zfp521 promoter reporter

    Plasmid

    #104188

    Purpose

    luciferase reporter to assay Zfp521 promoter activation

    Depositor

    Kathryn Hentges

    Article

    Yu et al Genesis. 2016 Oct;54(10):519-533. doi: 1

    Insert

    Zfp521 promoter - 1 Kb (Zfp521 Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    none

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pISlin41w(s) (lin-41 microRNA wt reporter)

    Plasmid

    #11906

    Depositor

    David Bartel

    Article

    Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

    Insert

    lin-41 miRNA target sequence (lin-41 Nematode)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. TP901 GFP BAC Reporter

    Plasmid

    #78216

    Purpose

    synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

    Insert

    synthetic circuit
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1 2xFLAG-2xSTREP EMI1

    Plasmid

    #158947

    Purpose

    Express human EMI1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    EMI1 (FBXO5 Human)
    

    Use

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFUGW-GFP-2-cut reporter

    Plasmid

    #131669

    Purpose

    Lentiviral plasmid that expresses a GFP-based reporter for monitoring CRISPR based precise end-joining.

    Depositor

    Alberto Ciccia

    Article

    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

    Insert

    GFP-2-cut reporter
    

    Use

    Lentiviral

    Tags

    P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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