We narrowed to 6,177 results for: nls

1. pPHR-EYFP-NLS

   Plasmid

   #103820

   Purpose

   Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-EYFP-NLS (CRY2 Mustard Weed)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-QUAS5x:GFPNLS-SV40pA

   Plasmid

   #155115

   Purpose

   Middle entry vector containing QUAS5x element upstream of GFP-NLS.

   Depositor

   Bret Pearson

   Article

   Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

   Insert

   QUAS5x:GFPNLS
   

   Use

   Gateway middle entry vector

   Promoter

   QUAS5x-E1b

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS-foxc1a-2a-nlstdTomato

   Plasmid

   #132970

   Purpose

   full-length of foxc1a in frame with viral 2a peptide and nuclear localized tdTomato in pCS expression vector

   Depositor

   Nathan Lawson

   Article

   Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

   Inserts

   tdTomato
   foxc1a (foxc1a Zebrafish)
   

   Tags

   NLS and myc

   Expression

   Mammalian

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. MS2_adRNA-MCP_ADAR2 DD_NLS

   Plasmid

   #124704

   Purpose

   Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NLS

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   MCP_ADAR2 DD_NLS
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. 7xNLS-pMJ915v2-sfGFP

   Plasmid

   #88922

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Inserts

   Cas9
   T7
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. RPL25NLS-GFP (101)

   Plasmid

   #24038

   Depositor

   Michael Rout

   Insert

   RPL25 (RPL25 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Only bp 1-135 (aa 1-45) of RPL25

   Available Since

   March 10, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pST1374-Lb-Cpf1-NLS

   Plasmid

   #107680

   Purpose

   Expresses LbCpf1 in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   LbCpf1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-NLS-tdMCP

   Plasmid

   #86245

   Purpose

   Middle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP

   Depositor

   Florence Marlow

   Article

   Campbell et al Development. 2015 Apr 1;142(7):1368-74.

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   NLStdMCP

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_hSyn_TALEBB(HD)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

   Plasmid

   #47450

   Purpose

   AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   TALE BB(N136_0.5HD_C63)-SID4X
   

   Use

   AAV; Tale

   Tags

   2A_phiLOV2.1

   Expression

   Mammalian

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available Since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. PS-CFP2-PCNA-19-SV40NLS-4

    Plasmid

    #57245

    Purpose

    Localization: Membrane Scaffold, Excitation: 400 / 490, Emission: 468 / 511

    Depositor

    Michael Davidson

    Insert

    PCNA-19 SV40NLS (PCNA Human)
    

    Tags

    PS-CFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA-NLS-ZapCmR1.1

    Plasmid

    #59011

    Purpose

    Nuclear zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NLS-ZapCmR1.1 genetically encoded Zn(II) sensor
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available Since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pmCherry-3xNLS-OptoJNKi

    Plasmid

    #89740

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO_AU1.HSV.VSVg_mCherry-NLS

    Plasmid

    #178225

    Purpose

    Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

    Depositor

    Brian Brown

    Article

    Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

    Insert

    nPC Tagged mCherry-NLS
    

    Use

    Lentiviral

    Tags

    AU1.HSV.VSVg and Nuclear Localization Signal

    Promoter

    eF1a

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol1-UAS-nlstdTomato

    Plasmid

    #67693

    Purpose

    Tol1 expression plasmid with UAS-driven myc tagged nlsTdTomato

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    6xMTnlsTomato
    

    Tags

    6xMyc-NLS

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2+ cMyc-oDam_f_GFPoNLS

    Plasmid

    #85818

    Purpose

    iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    cMyc oDam_f_GFPoNLS
    

    Tags

    cMyc and oNLS (optimized nuclear localization sig…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMpGWB102-Citrine-NLS

    Plasmid

    #79136

    Purpose

    Binary vectors for expression of Citrine fluorescent protein with a nuclear localization signal in plants

    Depositor

    Yutaka Kodama

    Article

    Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

    Insert

    Citrine-NLS
    

    Tags

    NLS (Nuclear Localization Signal)

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    Aug. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCVpuro-F-Gcn5-NLSm

    Plasmid

    #63708

    Purpose

    retroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    Gcn5 (Kat2a Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    mutated K425, K427 and K438 to R (arginine)

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pAAV_hSyn_TALEBB(NI)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid

    #47452

    Purpose

    AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE BB(N136_0.5NI_C63)-SID4X
    

    Use

    AAV; Tale

    Tags

    2A_phiLOV2.1

    Expression

    Mammalian

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_hSyn_TALEBB(HD)-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid

    #47446

    Purpose

    AAV TALE synthesis backbone with HD half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    TALE BB(N136_0.5HD_C63)-VP64
    

    Use

    AAV; Tale

    Tags

    2A_GFP

    Expression

    Mammalian

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pN1-NLS-Q7mu

    Plasmid

    #129314

    Purpose

    Positive control for ATP measurement, nucleus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

    Insert

    QUEEN-7mu
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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