We narrowed to 6,501 results for: nls

Showing: 741 - 760 of 6501 results

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L1P17

Plasmid

#195922

Purpose

Level 1 PhiC31 cassette with constitutive promoter: pUBQ10-PhiC31-NLS-tUBQ1

Depositor

Jennifer Nemhauser

Publication

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pUBQ10-PhiC31-NLS-tUBQ1

Expression

Plant

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L1P22

Plasmid

#195925

Purpose

Level 1 PhiC31 cassette with constitutive promoter: p35S-PhiC31-NLS-tUBQ1

Depositor

Jennifer Nemhauser

Publication

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

p35S-PhiC31-NLS-tUBQ1

Expression

Plant

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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L1P78

Plasmid

#195939

Purpose

Level 1 pARF19 reporter : pARF19-mScarlet-NLS-tRBCS

Depositor

Jennifer Nemhauser

Publication

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pARF19-mScarlet-NLS-tRBCS

Expression

Plant

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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yWL229 MCP-GFP-eRF3

Plasmid

#197053

Purpose

pUBC-NLS-HA-2xMCP-1xGFP-eRF3

Depositor

Robert Singer

Publication

Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

Insert

pubc-nls-ha-MCP-GFP-eRF3

Use

Lentiviral

Expression

Mammalian

Promoter

ubc

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBK-EE-BE3

Plasmid

#85175

Purpose

Expresses EE-BE3 in mammalian cells

Depositor

David Liu

Publication

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EE-BE3

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5_NLS-Halo-eGFP-BASU

Plasmid

#218449

Purpose

expressed the NLS-Halo-eGFP-BASU fusion protein via stable Flp-In integration

Depositor

Thorsten Stafforst

Publication

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

NLS-Halo-eGFP-BASU

Expression

Mammalian

Promoter

CMV

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pPD684 VPR-FRB (no NLS)

Plasmid

#138851

Purpose

Constitutive expression of VPR-FRB (no NLS) under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-FRB (no NLS)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPD683 VP64-FRB (no NLS)

Plasmid

#138850

Purpose

Constitutive expression of VP64-FRB (no NLS) under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP64-FRB (no NLS)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pPD682 VP16-FRB (no NLS)

Plasmid

#138849

Purpose

Constitutive expression of VP16-FRB (no NLS) under the CMV promoter.

Depositor

Joshua Leonard

Publication

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-FRB (no NLS)

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMEH305 HA NLS SpCas9 3x high affinity MS2 loops

Plasmid

#168216

Purpose

Mammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tag

Depositor

Joshua Leonard

Publication

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

SV40 NLS SpCas9

Use

Lentiviral

Tags

HA, SV40 NLS, and nucleoplasmin NLS

Expression

Mammalian

Promoter

CMV

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pHW522 (Prab-3::cGAL-C::let-858 3'UTR)

Plasmid

#107130

Purpose

cGAL-C driver under the control of C. elegans rab-3 promoter

Depositor

Paul Sternberg

Publication

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::gp41-1-C-intein::cGAL(AD)

Expression

Worm

Promoter

C. elegans rab-3 promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFS462

Plasmid

#89065

Purpose

his7 integration plasmid with Z3EVpr:GFP

Depositor

Nick Rhind

Publication

Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Z3EV promoter GFP-NLS leu1 C-terminal 300 nt

Use

S. pombe his7 integration vector

Promoter

Z3EV

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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NucCKAR

Plasmid

#14869

Depositor

Alexandra Newton

Publication

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

C kinase activity reporter

Tags

CFP, NLS, and YFP

Expression

Mammalian

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
KL301: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-p300core

Plasmid

#121839

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS/p300core scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+ Gal4-GBP2-IRES-GBP7-p65

Plasmid

#50020

Purpose

Multi-purpose expression of a GFP-dependent transcription factor

Depositor

Connie Cepko

Publication

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD

Use

Multi-purpose expression

Promoter

CMV-immediate early promoter and SP6 promoter

Available since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

Plasmid

#228400

Purpose

Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Publication

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

NES/PKI-mNeonGreen2x-NLS/FUS

Expression

Mammalian

Promoter

CMV

Available since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-NLS/cMyc-AcGFP2x-NES/iKB2-P2A-Puro

Plasmid

#228398

Purpose

Encodes NLS/cMyc-AcGFP2x-NES/iKB2 which is a tandem of AcGFP with an N-terminal cMyc NLS and a C-terminal iKB2 NES that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Publication

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

NLS/cMyc-AcGFP2x-NES/iKB2

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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ABE8.20-m

Plasmid

#136300

Purpose

expresses ABE8.20-m in mammalian cells

Depositor

Nicole Gaudelli

Publication

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABE8.20-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pGinB-8X(GGS)-dCas9-FLAG-NLS

Plasmid

#81205

Purpose

CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

Depositor

David Liu

Publication

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pGinB-8X(GGS)-dCas9-FLAG-NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pdCas9-PBE

Plasmid

#98161

Purpose

Expresses CRISPR base editor in protoplasts

Depositor

Caixia Gao

Publication

Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

Insert

NLS-APOBEC1-XTEN-dCas9-UGI-NLS

Expression

Plant

Promoter

Ubi

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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