We narrowed to 82,376 results for: TRI
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Plasmid#244845PurposeMammalian expression of Flex-Cas12a cytosine base editorDepositorInsertFlex-dCas12a-CBE
UseCRISPRTags2xSV40 NLS-APOBEC3A and Nucleoplasmid NLS-UGI-SV4…ExpressionMammalianMutationG146R/R182V/D535G/S551F/D665N/E795Q/D832APromoterCMVAvailable SinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMXs-KDM4A
Plasmid#188233PurposeExpress human KDM4A in mammalian cellsDepositorAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pX330 _CRISPIE donor B9
Plasmid#172850PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B9 (Zhong et al, eLife 2021), mEGFP translational phase (2-2), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and CRISPIE designer exon (phase 2-2) encoding mEGFP
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGH00.0126
Plasmid#64257PurposeBinary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformationDepositorInsertsEGFP
neomycin phosphotransferase II
UseBinary agrobacterium vector for plant transformat…ExpressionPlantPromoterE12-omegaAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330_VCL sgRNA / hSpCas9
Plasmid#172837PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-MRE11-H63S
Plasmid#82035PurposePlasmid for constitutive or doxycycline-inducible expression of H63S mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdRBFC-B2-YN
Plasmid#74721PurposeExpress C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labelingDepositorInsertInfluenza A virus NS1 protein, partial
UseDouble-stranded rna labelingTagsHA and YFP N-terminal half domainExpressionPlantPromoterCaMV 35SAvailable SinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
GEF-H1 FLARE212.pa
Plasmid#134930PurposeGEF-H1 biosensor that does not have GEF activity.DepositorInsertGEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
TagsNone.ExpressionBacterial and MammalianPromoterCMV and T7Available SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdRBFC-B2-YC
Plasmid#74722PurposeExpress C-terminal YC fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labelingDepositorInsertInfluenza A virus NS1 protein, partial
UseDouble-stranded rna labelingTagsFLAG and YFP C-terminal half domainExpressionPlantPromoterCaMV 35SAvailable SinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHLuc (Antares-SEPLuc C1A4E+6)
Plasmid#141089Purposefinal optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosisDepositorInsertAntares-T2A-puromycin-IRESv24-SEP-C1A4E+6
ExpressionMammalianPromoterCMVAvailable SinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
GEF-H1 FLARE191.pa
Plasmid#134931PurposeGEF-H1 biosensor that has GEF activity.DepositorInsertGEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
TagsNone.ExpressionBacterial and MammalianPromoterCMV and T7Available SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWF079_LVt-EF1a-TCRGibson_humanTRAC
Plasmid#233448PurposeGibson assembly of TCRB-P2A-TCRaDepositorTypeEmpty backboneUseLentiviralAvailable SinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-WHaloCaMP1a
Plasmid#205310PurposeProduction of AAV particles encoding WHaloCaMP1a from the CaMKII promoterDepositorInsertWHaloCaMP1a
UseAAVAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-VARNAM2-Kv2.1PR
Plasmid#195524PurposeRed fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertVARNAM2-Kv2.1 proximal restriction sequence
UseAAVExpressionMammalianMutationVARNAM SI linkerPromoterSynapsinAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
011LP-pAAV-TNT4-EmGFP-pA
Plasmid#120280PurposeAAV vector for cardiomyocyte specific expression of Emerald Green Fluorescent Protein using cardiac troponin T promoterDepositorInsertEmGFP
UseAAVTags3XFlag and HAPromoterTNT Cardiac Troponin T promoterAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
mouse CENP-B-EGFP
Plasmid#107264Purposemouse CENP-B-EGFP in pIVT vector for in vitro transcriptionDepositorAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330_TOMM20 sgRNA / hSpCas9
Plasmid#172836PurposeMammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-R-GECO1
Plasmid#32465DepositorInsertR-GECO1.0
UseLab constructedTags6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…ExpressionBacterialMutationSubstitutions relative to the mApple-derived anal…Available SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor
Plasmid#12153DepositorAvailable SinceJuly 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
AAT-PB-CG2APtk
Plasmid#86003Purposehomology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassetteDepositorInserteGFP_T2A_puro_dTK
UsePiggybac exciseableExpressionMammalianPromoterCAGAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330_TUBA1B sgRNA / hSpCas9
Plasmid#172834PurposeMammalian expression of a sgRNA targeting the intron 1 of TUBA1B (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of TUBA1B under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-DDdCas9VP192-T2A-EGFP-ires-puro
Plasmid#69534PurposeDHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.DepositorInsertDD-dCas9VP192-T2A-EGFP
TagsC-term T2A-EGFP and DHFR Destabilised DomainExpressionMammalianPromoterCAGAvailable SinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet-I3-NCwt-SYFP2
Plasmid#189765PurposeDual expression vector for bacteria and mammalian cells producing mScarlet-I3-NCwt-SYFP2 fusion protein for FRET analysisDepositorInsertmScarlet-I3-NCwt-SYFP2
Tags6xHisExpressionBacterial and MammalianPromoterCMV & RhaAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shRFP
Plasmid#110940PurposeTet-inducible shRNA targeting RFPDepositorInsertshRFP
UseLentiviral and RNAiPromoterH1/TOAvailable SinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330_CALR sgRNA / hSpCas9
Plasmid#172838PurposeMammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-HOXA9
Plasmid#51302PurposeExpression of HOXA9 after doxycycline administrationDepositorAvailable SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-CAR-GECO1
Plasmid#46022PurposeCarmine intensiometric genetically encoded Ca2+-indicators for optical imagingDepositorInsertCAR-GECO1
Tagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationR-GECO1 E163V/I166V/V174T/M176I/F222I/A302PPromoterCMVAvailable SinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EF1a-2A-ZsGreen
Plasmid#231985PurposeEmpty backbone for expression of GoI with Bi-cistronic expression ZsGreenDepositorInsert2A-ZsGreen
UseLentiviralAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-DEST-MatryoshCaMP6s
Plasmid#100025PurposeMammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
TagsV5 Epitope TagExpressionMammalianPromoterCMVAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only