We narrowed to 30,760 results for: PLE

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pNIR-Fb6m

Plasmid

#184680

Purpose

NIR-Fb to spike-Cov-19 RBD

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb6m (S Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-ATXN1_L588D

Plasmid

#48194

Purpose

Expresses L588D mutated Ataxin1

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Ataxin1 (ATXN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-ATXN1_L588A

Plasmid

#48193

Purpose

Expresses L588A mutated Ataxin1

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Ataxin1 (ATXN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-ATXN1_R665A

Plasmid

#48195

Purpose

Expresses R665A mutated Ataxin1

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Ataxin1 (ATXN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-PPHLN1 ts3

Plasmid

#115879

Purpose

PPHLN1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

PPHLN1 shRNA (PPHLN1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-MPHOSPH8 ts1

Plasmid

#115874

Purpose

MPHOSPH8 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

MPHOSPH8 shRNA (MPHOSPH8 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Lhx2

Plasmid

#74337

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Lhx2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

Insert

LIM homeobox protein 2 (Lhx2 Mouse)

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only
DYNLT1

Plasmid

#132528

Purpose

Human TCTEX-1, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLT1 (DYNLT1 Human)

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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YFP(1-158) in pcDNAI/Amp

Plasmid

#60888

Purpose

This vector attaches YFP(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

Insert

YFP(1-158)

Expression

Mammalian

Mutation

Met was substituted for Gln-69.

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pRS406 ATG1-ATG17-3'UTR

Plasmid

#166848

Purpose

Expresses Atg1 with ATG17 3'UTR in yeasts.

Depositor

Daniel Klionsky

Article

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Promoter

ATG1 Promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168169

Purpose

Expression of YFP-BeCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Tags

YFP

Expression

Worm

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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mr in pUASP

Plasmid

#112981

Purpose

P element vector for transposon insertion of mr (APC2) into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Kashevsky et al Proc Natl Acad Sci U S A. 2002 Aug 20;99

Insert

mr (APC2) (mr Fly)

Use

P insertion vector

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-MUS2-1246-vpr

Plasmid

#115841

Purpose

Encodes codon optimized 3xFLAG-SIVMUS21246 Vpr (EF070329) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMUS21246 Vpr (EF070329)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-GRV vpr

Plasmid

#115827

Purpose

Encodes codon optimized 3xFLAG-SIVGRV Vpr (M66437) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVGRV Vpr (M66437)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-GSN-CN71 vpr

Plasmid

#115830

Purpose

Encodes codon optimized 3xFLAG SIVGSN-CN71 Vpr (AF468658) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVGSN-CN71 Vpr (AF468658)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-DEN vpr

Plasmid

#115829

Purpose

Encodes codon optimized 3xFLAG SIVDEN Vpr (AJ580407) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVDEN Vpr (AJ580407)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-AGM-MAL vpr

Plasmid

#115828

Purpose

Encodes codon optimized 3xFLAG-SIVAGM Vpr (MAL_ZMB) (LC114462) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGM Vpr (MAL_ZMB) (LC114462)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-ASC vpr

Plasmid

#115824

Purpose

Encodes codon optimized 3xFLAG-SIVASC Vpr (KJ461715) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVASC Vpr (KJ461715)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-DEB vpr

Plasmid

#115823

Purpose

Encodes codon optimized 3xFLAG-SIVDEB Vpr (FJ919724) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVDEB Vpr (FJ919724)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-LST vpr

Plasmid

#115818

Purpose

Encodes codon optimized 3xFLAG-SIVLST Vpr (AF188116) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVLST Vpr (AF188116)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-MND1 vpr

Plasmid

#115817

Purpose

Encodes codon optimized 3xFLAG-SIVMND1 Vpr (M27470) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMND1 Vpr (M27470)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Ago3m

Plasmid

#21531

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago3 (AGO3 Human)

Tags

GFP

Expression

Mammalian

Mutation

missing aa757-823, the C-termianl 66aa of PIWI do…

Available Since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only
pDGE660

Plasmid

#153258

Purpose

M4E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCup_C

Plasmid

#146041

Purpose

Insect Expression of DmCup

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmCup (cup Fly)

Expression

Insect

Mutation

P418S

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-hRIP3-FL V460P

Plasmid

#61379

Purpose

Expresses Human full length RIPK3 with V460P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V460Pmutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V460 to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL V458P

Plasmid

#61377

Purpose

Expresses Human full length RIPK3 with V458P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V458Pmutation (RIPK3 Human)

Tags

EYFP

Expression

Mammalian

Mutation

changed V458 to P

Available Since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
Ets2DBM

Plasmid

#28129

Depositor

Pumin Zhang

Article

Li et al Nat Cell Biol. 2008 Sep . 10(9):1083-9.

Insert

Ets2 (ETS2 Human)

Use

Mouse Targeting and Retroviral

Expression

Mammalian

Mutation

The putative destruction box (RGTL) residues 20–2…

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only
C513-E07: attB5r-<p10p-polyhedrinp>-attB1r

Plasmid

#162949

Purpose

Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pR6Kg-Omega2

Plasmid

#165865

Purpose

Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCRII-Sema4c-ISH-2kb

Plasmid

#68031

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4C (Sema4c Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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