We narrowed to 9,977 results for: Uty

1. pJUMP27ts[mCherry]-1A(lacZ)

   Plasmid

   #126980

   Purpose

   Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number). Constitutive mCherry in downstream secondary site.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   RepA A56V thermosensitive mutation

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBGSA FPR1

   Plasmid

   #62600

   Purpose

   Constitutive expression of FPR1 in mammalian cells

   Depositor

   Heini Miettinen

   Article

   Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

   Insert

   FPR1 (FPR1 Human)
   

   Expression

   Mammalian

   Promoter

   SRalpha

   Available Since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pSB1A3-AD010

   Plasmid

   #116851

   Purpose

   Expresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010

   Depositor

   Friedrich Simmel

   Article

   Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

   Inserts

   AraC
   alpha-HL
   mScarletI
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCXLE-dCas9GFP-shP53

   Plasmid

   #102906

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9GFP-shP53
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. ptagBFP-LacI-NLS-VP16

   Plasmid

   #103837

   Purpose

   Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   tagBFP-LacI-NLS-VP16
   

   Expression

   Mammalian

   Promoter

   SV2

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG-RARa-VP16

   Plasmid

   #185559

   Purpose

   Ubiquitous expression of constitutively active Retinoic Acid Receptor alpha

   Depositor

   Connie Cepko

   Article

   Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

   Insert

   RARa-VP16 (Rara Mouse)
   

   Tags

   VP16

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. P16ΔS:S10-(MCS)-3xHA

   Plasmid

   #108183

   Purpose

   Binary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   P16ΔS

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACBB-TetT-LVA

   Plasmid

   #32555

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   TetR
   

   Use

   Synthetic Biology

   Tags

   LVA tag

   Expression

   Bacterial

   Promoter

   Consitutive lac

   Available Since

   Nov. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-pEF1a-rtTAc

   Plasmid

   #172121

   Purpose

   Constitutive expression of InteinC-rtTAC

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   InteinC-rtTAc
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. LUC56 (Luc/UGA/SP)

    Plasmid

    #112147

    Purpose

    Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

    Depositor

    Paul Copeland

    Article

    Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26

    Insert

    Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Rat, Photinus pyralis)
    

    Expression

    Mammalian

    Mutation

    Cysteine 258mutated to selenocysteine (U)

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. IRE-nonOpt-TREAT

    Plasmid

    #196922

    Purpose

    The codon optimized Renilla coding sequence in the IRE-TREAT reporter was replaced by non-optimal Renilla containing 93 synonymous codon substitutions.

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    non-optimal Renilla luciferase
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSIBR005

    Plasmid

    #177668

    Purpose

    Constitutive expression of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMD19T-psba1-Ppsba2-dCas9-SpR

    Plasmid

    #73220

    Purpose

    Contains dCas9 from S. pyogenes under constitutive promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

    Depositor

    Paul Hudson

    Article

    Yao et al ACS Synth Biol. 2015 Dec 28.

    Insert

    dCas9 from S. pyogenes
    

    Tags

    c-myc

    Expression

    Bacterial

    Mutation

    Silent mutation at bp 1341 A->C to remove an E…

    Promoter

    PpsbA2

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEAST2-sfGFP

    Plasmid

    #126575

    Purpose

    Strong constitutive expression of super-folder GFP for cell-free expression

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. SERA5-bio-His

    Plasmid

    #50817

    Purpose

    Expresses enzymatically monobiotinylated full length SERA5

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    SERA5
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXD70LacZ3-Pcsd-LacZ

    Plasmid

    #191619

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcsd promoter-lacZ, E. lenta constitutive promoter

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSRP5-bio-His

    Plasmid

    #50805

    Purpose

    Expresses enzymatically monobiotinylated full length MSRP5

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    MSRP5
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAW-EGFP-4xlet-7 (pAW-AS27)

    Plasmid

    #40834

    Purpose

    miRNA sensor used in Drosophila cell lines

    Depositor

    Phillip Zamore

    Article

    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

    Insert

    Four target sites perfectly complementary to let-7
    

    Use

    Mirna reporter

    Tags

    EGFP

    Expression

    Insect

    Promoter

    Actin5C

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pMM532

    Plasmid

    #112529

    Purpose

    pZA1D-hrtR - HrtR expressed consitutively from promoter ProD with RBS2, p15a origin, AmpR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Insert

    HrtR
    

    Expression

    Bacterial

    Promoter

    ProD

    Available Since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWPI-NP1

    Plasmid

    #65060

    Purpose

    Constitutive expression of NP1

    Depositor

    Ramon Trullas

    Article

    Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

    Insert

    Neuronal Pentraxin 1 (Nptx1 Rat)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    * see comments

    Promoter

    EF1alpha

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only